جستجوی مقالات مرتبط با کلیدواژه "testosterone" در نشریات گروه "پزشکی"

Cisplatin (DDP) resistance remains a primary cause of chemotherapy failure and recurrence of non-small cell lung cancer (NSCLC). Abnormal high microsomal glutathione transferase 1 (MGST1) expression has been found in DDP-resistant NSCLC cells. This study aimed to explore the function and mechanism of MGST1 in DDP resistance of NSCLC cells.

The expression levels of target molecules were assessed by quantitative real-time polymerase chain reaction (RT-qPCR) and western blotting. Cell proliferation was evaluated by cell counting kit-8 (CCK-8) and colony formation assays. Ferroptosis was determined by malondialdehyde (MDA), glutathione (GSH), Fe2+, and reactive oxygen species (ROS) levels. The interaction between proteins was confirmed by Co-immunoprecipitation (Co-IP). The effect of MGST1 on DDP resistance was evaluated using the tumor xenograft assay in vivo. Immunohistochemical staining was performed to measure Ki-67 and p-H2A.X expression in tumor tissues.

MGST1 expression was higher, while arachidonate lipoxygenase 5 (ALOX5) expression was lower in DDP-resistant NSCLC patients and cells. MGST1 ablation sensitized NSCLC cells to DDP therapy through inducing ferroptosis. MGST1 protein directly interacted with ALOX5 protein to restrain ALOX5-triggered ferroptosis. Ferroptosis inhibitor or sh-ALOX5 reversed the promotive effect of MGST1 silencing on the DDP sensitivity of NSCLC cells. Finally, MGST1 depletion sensitized NSCLC cells to DDP therapy in nude mice in vivo.

MGST1 high expression contributed to DDP resistance of NSCLC cells by inhibiting ALOX5-induced ferroptosis. Our results provide a potential therapeutic target for overcoming DDP resistance in NSCLC patients.

Antioxidants reduce the harmful effects of reactive oxygen species (ROS) in sperm. Additionally, nanoliposomes can be used as an efficient new drug delivery system that mitigates the adverse effects of drugs. This study aimed to investigate the use of nanoliposomes for delivering drugs such as resveratrol, catalase, resveratrol-catalase, and testosterone to normozoospermic samples to minimize the side effects of ROS.

This study took place at Yazd Reproductive Sciences Institute in Iran from October 2018 to December 2019. It involved 450 samples divided into three groups. Each of the 15 normozoospermic sperm samples collected from men was divided into 10 parts and analyzed in three groups: free drugs, nanoliposomes, and a control group. The study analyzed sperm parameters, PRM2 (Protamine 2) and HSP70 (heat shock proteins) expression, as well as sperm acrosome reaction and DNA fragmentation before and after freezing.

Nanoliposomes improved sperm motility and viability before freezing (P<0.05). Free resveratrol-catalase reduced motility before freezing (P<0.05). PRM2 and HSP70 expression were lower in the blank and testosterone nanoliposome groups (P<0.0001). After freezing, nanocarriers and free testosterone improved sperm motility (P<0.05). Resveratrol and testosterone-loaded nanoliposomes increased sperm viability (P<0.01). Nanoliposomes caused less sperm DNA damage (P>0.05). Free catalase reduced PRM2 and HSP70 expression (P<0.0001).

Nanoliposomes can be used as an efficient drug delivery system to decrease ROS production or their side effects. Additionally, during freezing, they improve sperm viability and reduce DNA damage.

The rising use of herbal medicines raises concerns about their safety and effectiveness. This case report involves a 55-year-old male who developed shortness of breath and chest discomfort after using herbal substances and sildenafil for erectile dysfunction and infertility. He was diagnosed with a massive acute pulmonary embolism (PE) via CT angiography and treated with alteplase, followed by oral apixaban after discharge. The herbal substances included carob, ginger, red ginseng, and others. This case highlights the need to investigate how herbal remedies may affect hormone balance and contribute to cardiovascular risks, particularly PE.

Andropause is a condition that affects men as they age, similar to menopause in women. Unfortunately, there is limited research on this topic in Iran, and many men are unfamiliar with this concept. To address this knowledge gap, a study was conducted to investigate the attitudes and awareness of andropause among men over the age of 40 in Ardakan City.

This study was conducted on 281 men aged over 40 years from Ardakan city in 2019. The sampling method used was cluster sampling and data were collected through a researcher-developed questionnaire. The collected data was analyzed, using SPSS software version 18 and the statistical methods of variance, Pearson correlation, and regression were used. 

Men have a low level of awareness about andropause, and their attitude towards it is negative. The study found that the mean score of participants’ awareness about andropause was significantly impacted by age groups (P <0.05), education level (P <0.05) and occupation (P <0.05). In terms of attitude towards andropause, the mean score was significantly impacted by age groups (P <0.05) and occupation (P <0.05), but not by education level (P >0.05). The Pearson correlation coefficient shows that there is a statistically significant positive relationship between the mean score of awareness and the score of attitude.

The low awareness and negative attitudes towards andropause among men in Ardakan City emphasize the need for more education and awareness on this topic. Providing men with accurate information about the symptoms, causes, and management of andropause is crucial to help them make informed decisions regarding their health. By addressing the knowledge gap and promoting positive attitudes towards andropause, healthcare providers can enhance the overall well-being of men in this age group.

Melissa officinalis L. ( lemon balm) is one of the more widely cultivated medicinal and aromatic plants that has long been used in traditional medicine to treat many disorders. The present study investigates Melissa officinalis L. hydro-alcoholic extract regarding pituitary-gonadal axis in diabetic mice.

45 NMRI mice with a mean weight of 35.6±4.5 g were divided into five groups: control group (0.2 ml of physiological serum intraperitoneally injection), diabetic mice (without treatment), and three experiment groups (diabetic groups receiving 50, 100 and 200 mg/kg of Melissa officinalis extract intraperitoneally injection). Melissa officinalis extract was injected intraperitoneally for 14 days. At the end of the experiment, blood samples were taken to determine the biochemical indicators level (glucose, LH, FSH, and testosterone), and the left testicle was weighed and examined histologically.

The results showed that the lowest amount of glucose and the highest level of LH were observed in the treatment group at a dose of 100 mg/kg of Melissa officinalis extract. The highest level of FSH was observed at a dose of 200 mg/kg. Histological study of the testis showed a more favorable condition in the experimental group of 200 mg/kg of lemon balm extract (P<0.05).

It can be concluded that the use of Melissa officinalis extract is efficient in reducing glucose, improving the levels of LH, FSH, and testosterone in diabetic mice, affecting testicular weight, and improving testicular tissue indices and reproductive function of rats.

Most individuals who suffer from spinal cord injury (SCI) experience neuropathic pain, which currently has no effective treatment. In this study, we examined how testosterone affects neuropathic pain resulting from SCI. 

We administered three different doses of testosterone (4, 8, 16 mg/kg, intraperitoneal) to male rats after an electrolytic lesion of the spinothalamic tract. We then conducted behavioral tests, including open field and von Frey tests, within 28 days post-SCI. On day 28 after SCI, we analyzed spinal tissue using western blot to measure the levels of ionized calcium binding adaptor molecule 1 (Iba1), glial fibrillary acidic protein (GFAP), phospho-extracellular signal-regulated kinase (p-ERK1/2), and p-P38 at the injury site. 

The results showed that testosterone significantly improved both motor activity and mechanical allodynia compared to the SCI-only group. Testosterone also inhibited microglia and astrocyte activation. Furthermore, testosterone significantly decreased p-P38 and p-ERK levels.  

The findings indicate that testosterone may alleviate SCI-induced neuropathic pain by inhibiting the activation of astrocytes and microglia, as well as suppressing MAPK signaling pathways.

Zinc and vitamin E affect the metabolism of testosterone and inflammatory factors. We aimed to evaluate the effect of zinc and vitamin E supplementation on plasma testosterone levels and inflammatory markers in patients undergoing coronary artery bypass graft (CABG) surgery.

This study was a secondary analysis of a previously published randomized controlled trial in a subsample of male patients undergoing CABG surgery. Patients in the zinc-vitamin E group (n = 27) received oral zinc (120 mg) and vitamin E (1200 international units) one day prior to surgery, followed by 30 mg of zinc and 200 units of vitamin E per day for three weeks after surgery. Patients in the control group (n = 25) received a placebo. Plasma levels of total testosterone, cortisol, interleukin-6 (IL-6), and white blood cell toll-like receptor-4 (TLR-4) gene expression were determined at three-day and three-week intervals following surgery. Changes in these markers were analyzed using a repeated-measures analysis of variance.

A comparison of the groups revealed no significant difference in the concentration of plasma total testosterone levels (P = 0.059) or cortisol. Three weeks following the surgical procedure, a positive correlation was observed between the change in plasma zinc concentrations and the change in plasma testosterone levels (r = 0.32; P = 0.025). The administration of zinc and vitamin E supplements resulted in a reduction in plasma IL-6 levels on postoperative day 3 (P = 0.025), while no significant effect was observed in week 3 (P = 0.091). The expression of the TLR-4 gene in WBCs was found to be lower in the zinc-vitamin E group compared to the placebo group on day 3 (P = 0.051) and week 3 (P = 0.025).

The administration of zinc and vitamin E to patients undergoing CABG was associated with a relative improvement in postoperative inflammatory markers. Plasma zinc levels demonstrated a correlation with testosterone levels, suggesting a potential avenue for further research in these patients.

Claims have been made that more sexual liberalism in society than what generally existed in human history is necessary to promote societal tranquility and reduce violence. This research was conducted to investigate the real relationship between sexual behavior and aggression based on scientific data especially from the perspective of physiological psychology. The present study investigates the hypothesis that unrestrained sexual behavior leads to an escalation of behavior, rather than satiation, and aggression rather than calmness.

This study employed a theoretical review methodology and drew upon previous findings from neuropsychology, psychophysiology, laboratory research, and field studies.

The study's results indicate that uncontrolled sexual behavior can lead to an increase in Sexual desire instead of sexual satisfaction. This increase may be due to a positive feedback loop between the nervous and hormonal systems, specifically the Medial Preoptic Area (MPA) and testosterone. Experimental findings suggest that this physiological process may result in aggressive behavior.

This paper establishes that the intensification of sexual behaviors leads to increased violence and aggression in society, ultimately elevating levels of public insecurity. Therefore, limiting sexual behavior within the framework of the relationship of married couples may serve as a moderating factor for this phenomenon.

In endemic malarial nations, repeated use of antimalarial drugs has increased due to resistance, misuse, and unrestricted availability, which could contribute to infertility rates. Therefore, we investigated the effects of long-term repeated treatment with two commonly prescribed artemisinin-based combination therapies, artemether/lumefantrine (A/L) and artesunate-amodiaquine (A/A), on reproductive potential in mice. 

Sixty male mice were divided into three groups: control, A/L, and A/A treatment.  Mice underwent treatment for three consecutive days per week, and this regimen was repeated every two weeks for a total of six cycles. Sperm parameters were evaluated after the 1st, 2nd, 3rd, and 6th exposure cycles, after which treated male mice were paired with female mice for mating.

Sperm viability was significantly reduced by 21% (P<0.001) following the 6th exposure to A/L, whereas the 2nd, 3rd, and 6th exposures to A/A resulted in significant decreases in sperm viability of 26% (P<0.001), 12% (P<0.01), and 31% (P<0.001), respectively, compared to the control group. Treatment with A/A during the 3rd and 6th periods led to a significant decline (P<0.001) in sperm mass activity by 20% and 28%, respectively, compared to the control group. However, long-term therapeutic exposure to A/L or A/A did not affect testosterone levels, epididymal content, or the ability to impregnate female mice.

Long-term treatment with A/L or A/A did not affect testosterone levels or epididymal content. However, a decrease in sperm viability was observed, even though the mice remained fertile.

 We aimed to evaluate the effect of treatment with testosterone with and without bisphosphonates on bone density in patients with thalassemia major.

This Quasi-experimental study was performed on patients with thalassemia major. Four groups were included in the study 1: patients with hypogonadism who were treated with testosterone and alendronate (HTA group, n=11), 2: patients with hypogonadism who were treated with testosterone (HT group, n=12), 3: patients with hypogonadism who did not receive any treatment (H group, n=5), and 4: patients without hypogonadism who were treated with alendronate (NHA group, n=10). The status of bone density was assessed before and after interventions and compared across the study groups.

A total of 38 subjects with mean age of 22.46 ± 4.64 years were investigated.The mean difference of Z score of the lumbar region before and after the intervention in the four groups was -1.65±0.65, -26±0.48, 0.00±0.00 and -0.32±0.21 respectively, that there was a significant difference between the four groups (P < 0.001). The mean difference of Z score of the hip region before and after the intervention in the four groups were -1.35±0.58, -57±0.37, 0.00±0.00 and -0.32±0.21 respectively, that there was a significant difference between the four groups (P < 0.001).

Adding bisphosphonates to testosterone in thalassemia patients with hypogonadism will improve bone density as well as reduce pain intensity in these patients. Also, administration of bisphosphonates in thalassemia patients without hypogonadism can be very effective in improving bone density and bone pain in these patients.

Prenatal exposure to lipopolysaccharide (LPS) can lead to structural damage and CNS dysfunction. The present study aimed to investigate the protective effects of prenatal administration of pycnogenol (PYC) against the negative effects of bacterial LPS on anxiety-like behavior, gonadotropin and sex hormone serum levels, and sperm quality and quantity in the adult male offspring of NMRI mice.

Pregnant mice were randomly divided into four groups (n = 10 per group): 1. Saline group: received a single dose of saline as solvent of pycnogenol by gavage for 3 days on gestation days 16-18. 2. LPS group: received a single dose of LPS (20 µg/kg, subcutaneously) on gestation day 20. 3. PYC: received 200 mg/kg/day of pycnogenol by gavage for 3 days, intraperitoneally, on gestation days 16-18. 4. LPS + PYC: received a single dose of LPS (20 μg/kg) on gestation day 20 and pycnogenol (200 mg/kg/day) by gavage for 3 days on gestation days 16-18. After maturity/puberty in male pups (60 days old), the anxiety-like behavior test was performed. After the behavioral test, serum levels of gonadotropins (luteinizing hormone, LH, follicle stimulating hormone, FSH), testosterone hormone and sperm quality were assessed.

LPS administration increased anxiety-like behaviors and decreased serum LH and testosterone levels; however, PYC treatment reversed the negative effects of LPS to normal levels.

PYC treatment improves anxiety-like behavior and gonadotropin and testosterone secretions. Therefore, this substance can be used as a protectant and an aphrodisiac agent.

Central nervous system damage in multiple sclerosis (MS) leads to severe physical disability and neurological defects. Sexual dysfunction and infertility in patients with MS have often been neglected in previous studies. Aerobic exercise is suggested to improve circulating testosterone levels and sexual function. Therefore, the purpose of this study was to investigate the effect of aerobic exercise on sex hormone levels in a cuprizone rat model of MS.

In this experimental study, 30 male rats (aged 70 days, 154.55 ± 18.1 g) were randomly divided into five groups: MS, exercise-MS (EX-MS), MS-EX, EX-MS-EX, and normal control (control). MS was induced by feeding cuprizone pellets (0.2%) to the rats for six weeks. The exercise groups performed an aerobic exercise protocol on a treadmill five days/week for six weeks before and during the induction of the MS model. Serum testosterone, follicle stimulating hormone (FSH), and luteinising hormone (LH) levels were measured using the ELISA method with standard kits (ZellBio Germany). Luxol fast blue staining (LFB) of the corpora collosa were performed.

The results showed a significant decrease in the serum levels of testosterone, FSH, and LH in the MS groups compared to the control group (P<0.05). There was a significant increase in the serum levels of testosterone, FSH, and LH in the EX-MS-EX, and EX-MS groups compared to the MS group (P<0.05).

Aerobic exercise could improve the level of sex hormones in the cuprizone rat model of MS and may be used to attenuate sexual dysfunction in patients with MS.

Research has demonstrated a negative association between type 2 diabetes mellitus and male gonadal function, with reductions observed in serum levels of testosterone, follicle-stimulating hormone, and luteinizing hormone. Conversely, resistance training has been shown to positively influence these hormones in healthy men. This study aimed to investigate the effect of resistance training on blood glucose, testosterone, follicle-stimulating hormone, and luteinizing hormone levels in men with type 2 diabetes.

This semi-experimental study included 20 men with type 2 diabetes (aged 45-60 years) who were randomly assigned to the resistance training or the control groups (n=10 per group). The training group participated in an eight-week supervised program targeting major muscle groups. Blood samples were collected pre- and post-intervention to measure blood glucose, testosterone, luteinizing hormone, and follicle-stimulating hormone levels. Data were analyzed by SPSS 26 using the mixed ANOVA and an independent t-test.

The resistance training group demonstrated significant improvements (p<0.05) in blood glucose, testosterone, and luteinizing hormone levels compared to the control group and baseline measurements. No significant changes were observed in follicle-stimulating hormone (p>0.05).

Eight weeks of resistance training enhance blood glucose control and gonadal function in men with type 2 diabetes.

The purpose of the current study was to compare the testosteroneestradiol (T:E2) ratio in Toxoplasma gondii seropositive infertile men with seropositive and seronegative normozoospermic controls.

Totally, 200 men with normal virilization, 100 with idiopathic infertility and 100 normozoospermic men, were included. Participants underwent medical history assessment, physical examination, semen analysis, testing for T. gondii IgM/ IgG, and estimation of serum T:E2 ratios. Statistical comparisons were done using ttest and Chi-square with p<0.05 significance level.

Infertile cases were diagnosed with oligozoospermia (63%), oligoasthenozoospermia (34%), and oligoasthenoteratozoospermia (3%). Regarding anti-Toxoplasma IgG and IgM antibodies, among infertile men, 34 tested positive for IgG and 8 tested positive for IgM. Among cases tested positive for IgG antibodies, 13 (38.2%) had disturbed T:E2 ratios. Also, among the 12 IgG-positive controls, 5 (41.7%) had disturbed T:E2 ratios (p=0.834). However, only 2 out of the 83 seronegative controls (2.5%) had disturbed T:E2 ratios (p<0.001). Furthermore, 6 out of 8 IgM-positive cases had altered T:E2 ratios, compared to 3 out of 5 IgM-positive controls (p=0.568) and 2 out of 83 seronegative controls (p<0.001). The T:E2 ratio was significantly lower (8.68±1.95) among IgM-positive and higher (13.04±3.78) among IgG-positive cases when compared to seronegative controls (10.45±0.54) (p<0.001). There were no significant differences in T:E2 ratios between infertile men with positive IgM or IgG serology and the control group with the same serology.

A substantial number of infertile men with toxoplasmosis showed disrupted T:E2 ratios, highlighting the significance of anti-T. gondii-IgG testing in individuals with abnormal ratios.