جستجوی مقالات مرتبط با کلیدواژه "vibrio" در نشریات گروه "پزشکی"
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BackgroundDumpsites have the potential to serve as reservoirs for various medically important bacteria and their virulence and resistance gene markers. For Vibrio spp., numerous genes associated with virulence have been identified in environmental strains. Due to the specific growth requirements of Vibrio spp., such strains can often be overlooked. This study aimed to assess the potential of Vibrio spp. isolated from two dumpsites in Port Harcourt, Nigeria, to serve as reservoirs of virulence and resistance genes.MethodsThe soil samples were evaluated for the presence of Vibrio spp. following enrichment, using standard microbiological and biochemical test methods. DNA from Vibrio spp. was extracted using the boiling method, and isolates were tested for the presence of four resistance (sxt, strB, BlaTEM, and dfrA1) and four virulence (ctxA, hlyA, tcpA, and toxR) genes.ResultsThe study found a 40% occurrence of resistance genes and a 10% occurrence of virulence genes, with the strB streptomycin resistance gene being the most commonly detected (42%). Two of the virulence genes (ctxA and tcpA) were not detected. Seven of the test isolates exhibited multiple gene markers, with four gene markers present in each of two isolates.ConclusionOverall, the study revealed a generally low potential for Vibrio sp. isolated from the dumpsites in Port Harcourt, Nigeria, to act as reservoirs of virulence and resistance genes. Additionally, the study reported an absence of major virulence markers associated with V. cholerae. A concerning finding was the high occurrence (42%) of the strB gene among these environmental isolates.Keywords: Virulence, Vibrio, Waste Sites, Resistance Genes, Nigeria, Reservoir
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Collagenase is one the important enzyme, which is applied in varied fields ranging from tannery, food and cosmetic industries to clinical therapies. Currently, the commercially available collagenase enzyme has been produced by Clostridium histolyticum bacteria. In our study, in order to find new sources of collagenase producer, 30 collagenases from different species of Clostridium, Vibrio and Bacillus were evaluated from the view of phylogenetic relation, domain architecture and Physiochemical features. Totally our results indicate that the non-pathogenic C. novyi (NT) with the aliphatic index (80.68), instability index (27), pI (6.54), Mw (112.838 kDa) and two PPC domain could be suggested as potent bacteria for industrial production of collagenase.
Keywords: Bacillus, Collagenase, Clostridium, In silico features, Vibrio -
BackgroundThere are 4 different genera (i.e. Vibrio, Aliivibrio, Photobacterium, and Shewanella) in the new classification of bioluminescent bacteria. The mechanism of bioluminescence has yet to be fully elucidated. Therefore, the determination of physiological and genetic characteristics of bioluminescent bacteria isolated from different sources is very important. Pulsed-Field Gel Electrophoresis (PFGE) has the highest discriminatory power among the different molecular typing methods for the investigation of the clonal relationships between bacteria. For the PFGE analysis of bioluminescent bacteria, the NotI-HF™ is the method of choice among the restriction enzymes.ObjectivesThe present study aimed to determine genetic relatedness via PFGE in 41 bioluminescent bacteria (belonging to 10 different species) isolated and identified from various marine sources.Materials And MethodsDifferent bioluminescent bacteria (i.e. Vibrio gigantis, V. azureus, V. harveyi, V. lentus, V. crassostreae, V. orientalis, Aliivibrio logei, A. fischeri, Shewanella woodyi, and Photobacterium kishitanii) were analyzed by PFGE using the NotI-HF™ restriction enzyme. The whole DNA of the strains embedded into the agarose plugs was digested with enzyme at 37°C for 30 minutes. CHEF-Mapper PFGE system was used for electrophoresis and band profile of the strains for the NotI-HF™ restriction enzyme were analyzed by Bio-Profil-1D++ software (Vilber Lourmat) at 10% homology coefficient.ResultsAlthough all experiments were performed three times, four of forty-one bioluminescent strains (V. gigantis E-16, H-16 and S3W46 strains and A. fischeri E-4 strain) could not be typed by PFGE technique with NotI-HF™ enzyme. While only two strains (V. crassostreae H-12 and H-19 strains) were exhibiting same band pattern profiles (100% genome homology), thirty-six different PFGE band patterns were obtained. Pattern homologies changed between 66% - 92%, 73% - 83% and 49% - 100% for V. gigantis, V. harveyi and other strains, respectively.ConclusionsThe obtained results revealed that there has been a high rate of genetic diversity in bioluminescent strains isolated from Gulf of Izmir and V. lentus and V. crassostreae strains could be also bioluminescent for the first report. At the same time, PFGE analysis of bioluminescent bacteria including four different genera and ten different species were shown for the first time by this study. It is considered that data acquired by this study will contribute evolution and mechanism of bioluminescence to further works to be done.Keywords: Pulsed, Field Gel Electrophoresis, Bioluminescent, Aliivibrio, Shewanella, Vibrio, Photobacterium
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BackgroundSeveral hundred thousand people are infected with Vibrio cholera every year worldwide, with a case fatality rate over 2%. Periodic cholera outbreaks are of important health issues in Iran, especially in Sistan and Baluchistan province..ObjectivesIn this study, we evaluated cholera out breaks and related factors, during 2010-2013, to improve controlling the disease in this province..Materials And MethodsIn this study, all epidemiologic data about patients with Cholera, during 2010-2013, were evaluated. Afterwards the environmental determinants and management indices were assessed..ResultsThe largest outbreak occurred in 2013. The incidence rate was significantly higher in men than women (RR = 2.8), in foreign immigrants than the Iranians (RR = 44.1) and in rural population than the urban population (RR = 26.2). The major part of patients, especially the first cases, lived in areas with poor sanitation like immigrant camps or remote villages and did not have access to sanitary water..ConclusionsProblems like poor sanitation and sanitary water access limitation, in rural and remote areas and also in immigrant camps necessitate allocating more resources, better inter-institutional cooperation and more intense border travelling control in this province..Keywords: Outbreaks, Cholera, Vibrio
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زمینه و هدفویبریوها به عنوان عامل برخی از اختلالات گوارشی و خارج گوارشی شناخته شده اند، که معمولا در برخی از فصول سال به صورت اپیدمی ظاهر می شوند. از آنجایی که دریا به عنوان یکی از زیست بوم های مهم ویبریوها شناخته شده است، این تحقیق با هدف تعیین فراوانی انواع ویبریوها و تعیین مقاومت میکروبی آنها، در مناطق مختلف ساحلی جنوب کشور در فصول مختلف سال انجام شد.روش بررسیدر این مطالعه توصیفی – مقطعی، 600 نمونه آب برداشت شده از آبهای ساحلی شهر بندرعباس از نظر وجود سویه های ویبریو در دو فصل بهار و زمستان بررسی شدند. برای تشخیص اولیه، از محیط های آب پپتونه قلیایی و TCBS استفاده گردید و با انجام تست های بیوشیمیایی (اکسیداز، حرکت، اندول، KIA و سایر آزمون های بیوشیمیایی از جمله نمک 0%، 6%، VP و ONPG) تشخیص نهایی انجام شد.یافته هاویبریو هاروئی با 3/2%، ویبریو کلرا 7/1%، ویبریو پاراهمولیتیکوس 3/1%، ویبریو فورنیسی 2/1%، شیگلوئید و ویبریو متسچینوکووی 1%،، هیدروفیلا 5/0%، ویبریو ولنیفیکوس و ویبریو میمیکوس 3/0% و ویبریو فلوویالیس 2/0%، بیشترین فراوانی را به خود اختصاص دادند. همچنین برحسب فصول، 2/71% آلودگی مربوط به فصل زمستان و 8/28% مربوط به فصل بهار بود، که نشان می داد در زمستان موارد آلودگی بیشتر از فصل بهار بوده است.نتیجه گیریبا توجه به مطالعه محققین، جداسازی این گونه ها بعد از بارندگی، افزایش می یابد. در مطالعه حاضر نیز این پارامتر در جداسازی گونه های ویبریو تاثیر مثبت داشته است.
کلید واژگان: ویبریو, آبهای ساحلی, بیماری های روده ای, بندرعباس, ایرانBackground And ObjectivesVibrio species have been known as the cause of gastrointestinal and extraintestinal disorders, which usually appear epidemically in some seasons of the year. Since the sea has been known as one of the important ecosystems of Vibrio species, this study was carried out with the aim of determining the prevalence of Vibrio species and their microbial resistance in different coastal regions of the south of country in different seasons of year.MethodsIn this descriptive cross-sectional study, 600 water samples taken from the coastal waters of Bandar Abbas were evaluated in terms of presence of Vibrio strains in two seasons of spring and winter. For primary detection, alkaline peptone water and TCBS were used, and the final identification was carried out using biochemical tests (oxidase, motility, Indole ring, KIA, and other biochemical tests such as 0% NaCl, 6% NaCl, VP, and ONPG).ResultsV. harveyi (2.3%), V. cholerae (1.7%), V. parahaemolyticus (1.3%), V. furnissii (1.2%), V. metschnikovii (1%), P. shigelloides (1%), A. hydrophila (0.5%), V. vulnificus and V. mimicus (0.3%), and V. fluvialis (0.2%) had the highest prevalence. Also, in terms of the studied seasons, 71.2% of the contamination was related to winter season and 28.8% to spring season, which showed that the cases of contamination in winter was higher than in spring.ConclusionBased on the study of researchers, the isolation of these species increases after rain. In the present study, this parameter had a positive effect on the isolation of Vibrio species.Keywords: Vibrio, Coastal Waters, Intestinal Diseases, Bandarabbas, Iran
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