جستجوی مقالات مرتبط با کلیدواژه « آنتی اکسیدان تام » در نشریات گروه « پزشکی »

Exercise interventions and consumption of some supplements can lead to changes in the antioxidant system and oxidative stress of skeletal muscles. Based on this, the present study investigated the effect of four weeks of Nigella sativa oil supplementation and aerobic exercise on the serum levels of malondialdehyde and the total antioxidant capacity of inactive young women. For this purpose, 40 inactive young women with an average age of 22.23 ± 4.51 years and BMI of 25.46 ± 4.21 participated in the research. Prior to the treatment, they filled out a written consent form. Then, the subjects were randomly divided into four groups: control (N=9), exercise + placebo (N=10), supplement (N=10), and exercise + supplement (N=11). The aerobic exercise groups performed four weeks of aerobic exercise with an intensity of 50% to 80% of the maximum heart rate, three sessions a week. Also, the Nigella sativa oil supplement groups received 1000 mg of its capsule per day after lunch. Blood samples were collected to measure the serum level of malondialdehyde and total antioxidant capacity in two stages before and after 24 hours of the last training session in fasting conditions from antecubital vein. Statistical analysis was performed by paired t-tests and the analysis of covariance at a significance level of P<0.05. Results showed that there is a significant decrease in total antioxidant capacity values between the aerobic group with the two supplement groups (P=0.03) and supplement + exercise (P=0.03). Also, a significant decrease was shown after the test as was compared to the pre-test in the within-group values of malondialdehyde serum concentration in the supplement group (P=0.03). According to the results, we conclude that the aerobic exercises and Nigella sativa supplementation leads to a decrease in the total antioxidant capacity and it has no effect on the oxidative index.

The incidence of heart disease increase with age. Green tea is an antioxidant which can prevent cellular aging. Therefore, the present study aims to investigate the effect of 12 weeks of green tea extract consumption on total antioxidant capacity (TAC), malondialdehyde (MDA) levels and superoxide dismutase, glutathione peroxidase and catalase activity of old ratschr('39') heart tissue in response to acute exhaustive exercise.

32 male rats were randomly categorized into two groups of supplement and non-supplement each consisting of 16 rats. The supplement group received green tea extract for 12 weeks. At the end of the 12 weeks, each of these groups was further divided into two groups of rest and acute exhaustive exercise. The non-supplement with the rest group was called control group. The non-supplement with exercise group was called exercise group. Further, the supplement group with the rest was called supplement group and finally the last group was entitled supplement with exercise. After 12 weeks of taking green tea extract, one session of acute exhaustive exercise was held on the treadmill. Sandwich ELISA method was used to measure TAC and MDA content and superoxide dismutase, glutathione peroxidase and catalase activity

The results showed that 12 weeks of green tea extract consumption increased TAC and decreased MDA levels. Furthermore, increased superoxide dismutase, glutathione peroxidase and catalase activity in response to a bout acute exhaustive exercise.

According to the results of this study, 12 weeks of green tea extract consumption can increase TAC levels and increase the activity of antioxidant enzymes at rest and after acute exhaustive exercise.

 Despite the positive effects of high-intensity interval training (HIIT), these training cause oxidative stress due to high intensity. Therefore, the purpose of this study was to investigate the effect of 6 weeks of HIIT training by using Nano-Curcumin supplement on total antioxidant capacity and Malondialdehyde level in overweight girls.

The present study was a quasi-experimental study with a pretest-posttest design. Accordingly 48 overweight girl students with average age (21.78 ±0.94 years) and BMI (28.12±2.1 Kg/m2) entered were randomly and equal divided into four groups: training group (n=12), training-supplement (n=12), supplement (n=12) and control group (n=12). Supplemental groups consumed an 80 mg capsule daily of Nano-Curcumin. Training groups, three sessions per week for 6 weeks performed HIIT exercises with 85 to 95% of maximum heart rate. Before and after training and consumption the supplementation, Blood samples were obtained to measure Malondialdehyde indices and total antioxidant capacity.

In the present study, there was a significant decrease in Malondialdehyde levels (P = 0.009) and a significant increase in total antioxidant capacity (P = 0.01) in the training-supplement group while levels of Malondialdehyde increased significantly (P = 0.004) in the training group.

 It seems, HIIT training causes oxidative stress in overweight people, concomitant use of the supplement may possibly improve the total antioxidant capacity in overweight people.

During intense exercise reactive species produce oxygen that results in cell damage. It seems that the use of antioxidant supplements reduces the oxidative stress caused by such exercises. Therefore the purpose of this study was to investigate the effect of short-term colostrum supplementation on serum malondialdehyde levels and total antioxidant capacity induced by acute resistance in healthy men.

The statistical population of this study was all healthy students of Birjand University with age range of 20 to 24 years old. Ten of them were randomly selected as the statistical sample. The present study was carried out in a semi experimental and applied cross-sectional design with an experimental group (n=10) to control individual differences. Subjects of this research were resistance movement protocol, consisting of six movements in 10 replications with 80% 1Rm, one minute rest between the turns and two minutes of rest between the movements. The subjects were taken from the arterial vein before the beginning of the exercise program at each stage and immediately after the exercise program followed by a 12-hour fasting of 8 ml of blood. After 14 days of supplementation, resting was performed again after 5 minutes, and blood samples were taken from the subjects. From the day the protocol was administered to the subject for a period of two weeks, one day, using the powdered colostrum supplement in a dose of 20 grams that was taken with 200 ml of water. The bleeding process was repeated after two weeks of supplementation.

The concentration of malondialdehyde decreased significantly after colostrum supplementation after two weeks(p=0.001), Also serum total antioxidant capacity increased after colostrum supplementation after two weeks(p=0.007).

Due to the antoxidant properties of colostrom, and oxidative stress during sport activities and imbalance supplement to reduce and inhibition of oxidative stress.

Some herbs contain compounds with antioxidant activity and can be used to protect or cure damages caused by chemical toxins. The aim of this study was to evaluate the effects of carbon tetrachloride (CCl4) on total antioxidants in various tissues and Tanacetum parthenium impacts on reducing devastating effects of carbon tetrachloride.
A total of 42 male Wistar rats were divided into seven groups of six animals in each group: normal control, damaged control, three groups that treated with 40, 80, and 120 mg/kg of Tanacetum parthenium extract 14 days before CCl4 injection and two groups served as post-treatment groups that received 80 and 120 mg/kg extract 2, 6, 24, and 48 h after CCl4 injection. At the end of study the liver, kidney, testis, and heart were removed and then homogenized and then the antioxidant activity of the tissues assessed using FRAP method. Results were analyzed by one-way ANOVA test.
The results showed that the injection of carbon tetrachloride significantly decreasestotal antioxidant in both liver (p Protective effect of Feverfew against CCl4 induced damages is more effective in liver and kidney than testis and heart..

Background & Ma-al-Shaeerisanon-alcoholicbeer which usually consumed in the younger generation in Islamic countries. It is regarded as having antioxidant compounds and in additiontohavingthe desirable organoleptic properties. Therefore, thisstudy designed to determine the effect of non- alcoholic beer (Ma-al-Shaeer) intake on the total antioxidant capacity (TAC) levelsinyoung people.
Material & In an interventional study,50 students including; 25 girls and 25 boys were selectedas simple random. Then,4ml ofblood was takenfroman individual before and after consumption of 240 millitr Ma-al-Shaeer respectively .After separating the serum, total antioxidant levels was measured by Ferric Reducing Antioxidant Power (FRAP) method. Thespectrophotometric method was used for measuring the level sof uric acid and total thiols (Hu's method). Statistical analysis was done by software SPSS, version 17. It was used for determination mean, standard deviation, number and percent and also one-way ANOVA test for comparison of variables. P The mean age of samples 20±1.2 year. It was observed significant differenceinmean of total antioxidant level in subjectsbefore and after intervention (1312.3±301 μ mol /lvs.1374.4±345 μ mol/l) (P= 0.0001). But, there was no significant differenceinserumuricacidlevels of subjects. It was observed significant difference between mean serum levels of totalthiols of subjects in before and after intervention (0.42 ± 0.22 mg/ dl vs. 0.59 ± 0.18 mg/dl) (P= 0.0001). There was a significantpositive correlation between consumption of total antioxidant level and totalthiol of subjects in before(r=1, P Results showed thatnon-alcoholic drink Ma-al-Shaeer dramatically increased serum TAC and protein thiols in subjects. According to effect of antioxidant in health, it seems increase of antioxidant power in Ma-al-Shaeer can be analyzed which requires to more extensive investigation.

Pemphigus comprises a group of autoimmune life threading diseases characterized by cutaneous and mucosal blistering. Pemphigus vulgaris (PV) is the most common and important variant. Recent reports have revealed the relatively high incidence of PV in Iran. The onset or aggravation of many human diseases is attributed to oxidative stress and it has been suggested as one of the several factors responsible for etiopathogenesis of pemphigus.The present study was performed to assess the association of serum level of antioxidant enzyme glutathione peroxidase (GPx) and total antioxidant (TAS) and SOD (superoxide desmotas) with pemphigus vulgaris. This case-control study evaluated patients referring to Razi Dermatology Hospital in Tehran, Iran from January 2011 to November 2012. Thirty patients with early diagnose of PV, who had never been under treatment were involved in this study as the case group and the control group consisted of 30 age- and sex-matched healthy people in the same center. The diagnosis of PV was confirmed by histopathology and direct immunofluorescence. Serum was collected from subjects for evaluation of glutathione peroxidase and total antioxidant levels and comparison with controls. GPX, TAS and SOD activity was analyzed by spectrophotometry method. Independent samples t -test was applied to compare the means of continuous variables. Statistical analysis was performed using SPSS software. P ≤ 0.001 was considered significant. The mean serum level of glutathione peroxidase and total antioxidants was significantly lower in pemphigus patients. No statistically significant difference was observed between the groups in SOD levels of serum. The results of this study suggest that serum antioxidant level in patients with pemphigus has been changed and also showed that the change of TAS, GPX can have an important role in the Interactions of pemphigus.

Smoking is a harmful habit that causes adverse effects on oral health and plays the most important role in cancer, precancerous lesions and periodontal disease. Saliva is the first fluid that is exposed to cigarette smoke. Salivary antioxidant system plays an important role in its anti-cancer potential therefore; this study was designed to compare the antioxidant content of saliva of smokers and non-smokers. In this cross- sectional study unstimulated saliva of 50 male smokers and 50 male non-smokers who were free of oral disease were collected by spitting method and total antioxidant capacity of their saliva was evaluated by FRAP method. Data were analysed by SPSS software version 21 and independed t test was used to compare the two groups. The average total antioxidant capacity of saliva in smokers and non-smokers were 489.95±203.82 and 589.48±226.05, respectively which were significantly different (P=0.008). Based on the results, smoking can reduce the total antioxidant capacity of saliva.

The aim of this research is investigation of Cu/ Zn Superoxide Dismutase e nzyme of lymphocytic cell gene expression, total antioxidant status and oxidative stress changes following intensive exercise in young men athletes. This study was a semi-experimental research with a repeated measures design. 20 young men athletes (age range of 21-23 years) participated in this study after signing an informed consent form. Blood sample were collected in pre intensive exercise (grade: 5%, speed: 7/5 mile/h, time: 20 minutes) immediately and recovery (3 hours after exercise test), and Real time-polymerase chain reaction was used for evaluation of Cu/Zn SOD gene expression and autoanalyzer for other markers. H2O2 level and mRNA of Cu/Zn SOD were both increased, immediately and 3 hours after of exercise (p=0/012, p=0/014), (2.95 ± 0.84 and 3.37 ± 0.99) but this changes were not reported significant, but TAS levels areeffectively, raised only in recovery state (p=0/009) (0.86 ± 0.16). Intensive exercise increases oxidative stress markers and can weakens the immune system of men athletes, but they raisethe activity of antioxidant enzymes in response to threat of free radicals, so Cu/Zn SOD gene expression does not significantly increased.

Reactive oxygen species can affect many cellular functions through protein oxidation or initiation of lipid peroxidation cascade. The study was designed to investigate changes in erythrocyte glutathione peroxidase activity and plasma total antioxidant level due to Securigera Securidaca extract in diabetic rats. The present experimental study was carried out on 30 male rats scientifically named Ratus Norvegicus, including normal and diabetic groups. In addition, each group was divided into three subgroups (5 rats per each): a control group and two subgroups which received 100mg/kg and200 mg/kg of the extract. The rats received all the injections intraperitoneally for thirty days. After the termination of injection period, blood was drawn from the heart and glutathione peroxidase activity and plasma total antioxidant levels were assessed. Statistical differences were evaluated by ANOVA and Student's t- test. Glutathione peroxidase activity in diabetic subgroups treated at doses of 100mg/kg and 200mg/kg was heightened in comparison to the diabetic control subgroup(P=0.01 and P=0.004 respectively). Plasma total antioxidant level in diabetic subgroups treated at doses of 100 mg/kg and200mg/kg was increased compared to the diabetic control subgroup (P=0.005and P=0.035 respectively). Securigera Securidaca extract probably increases antioxidant defense in diabetic rats by making changes in glutathione peroxidase activity and plasma total antioxidant level.

Myrtus Communis, L. is a medicinal herb, which has antibacterial activity and is used against many pathogens. The present study was conducted to investigate the mechanism of antibacterial activity of the methanolic extract of this herb against E.coli K12 HB101. E.coli K12 HB101 containing plasmid pBR322 was cultured in Muler Hinton agar under different concentration (1.56-6.25 mg/ml) of Myrtus communis air-dried extract. The cells were lysed by repeated sonication and freeze -thawing. The catalase, and superoxide dismutase(SOD) activities, total antioxidant (TAS) and malondialdehyde determination were performed on the cell lysate. Plasmid DNA was purified and the electrophoretic behavior of the purified DNA grown under different concentration of the extract were compared. Various Concentrations (SIC) of the extract did not show any changes in the DNA topology. When the extract concentration in the culture increased from 1.56 to 6.25 mg/ml the catalase specific activity of the cell extract reduced from 20.47 ± 0.7 to 5.1 ± 2.9 unit/mg protein. Under the same condition, SOD activity was decreased from 63.86 ± 5.2 to 20.04 ± 1.6 units/mg protein. Total antioxidant capacity in the cell lysate significantly decreased in the test samples compared to the control cultures (18.07 ± 3.1 vs 6.19 ± 2.1 mmole/L). Malondialedhyde concentration was elevated from 23.09 ± 7.6 to 49.92 ± 3.4 m g/mg protein of the cell lysate. The extract of Myrtus communis persumabably does not change the DNA topology of E.coli as judged by excision of plasmid pBR322. The results however showed that, Free radicals are one of the major causes of the antibacterial action of this medicinal herb.