جستجوی مقالات مرتبط با کلیدواژه « تست آنتی بیوگرام » در نشریات گروه « پزشکی »

Group B Streptococcus (GBS) or agalactia is an important cause of infection among the early newborns. The bacterium is transmitted from mother to child and most often leads to infant death. The goal of this study was to detect GBS among clinical samples of pregnant women in Kermanshah province, using Colony PCR and standard microbiological culture and then to compare the phenotypic and genotypic methods against each other.
One hundred cases aged at 30- 38 weeks of life were selected during 4 months from April 2014 on at health centers of Kermanshah province. The samples were taken from the women’s vaginal secretions and tested by standard culture using basic culture medium Todd- Hewitt broth, blood agar and Colony- PCR targeting scpB gene.
Findings: The results showed phenotypic test among 100 samples, 5 (5%) as carriers of group B streptococci, compared to 7 patients (7%) by Colony PCR test.
Conclusion & According to our study, the rate of GBS incidence is high in women of Kermanshah. Furthermore, the Colony PCR method that eliminates DNA extraction process can be a rapid, reliable and low cost method to detect the carrier.

Nano-fiber is a high-performance anti-microbial biocompatible polymer. And was used for Coverage of superficial wounds, such as burn wounds. These nanofibers can be a suitable alternative to conventional bandage.in this research the preparation of nanofibers and anti-bacterial properties has been studied.

Polymer solution of chitosan/ polyethylene oxide/ henna extract were prepared and using electros pining machine for nanofibers prepared and Its antibacterial properties was evaluated with Hinton agar medium containing the bacteria Escherichia coli and staphylococcus.

The diameter of nanofibers were of 130 to 300 nm with SEM microscopy.the result of antibiogram tests with disks containing antibiotics and nanofibers indicates the zone of no growth bacterial growth on medium. As regards the prevent infection were the most important point in the treatment of burns and wounds so bandage containing nanofibers with anti- bacterial properties can be used instead of using antibiotics with numerous side effects .

The results of Antibiogram test indicate the high antibacterial properties of prepared Nano-fibers this is due to the antibacterial properties of chitosan and extracts Henna.

As an inappropriate antibiotic administration, the emergence of drug resistance in pathogens associated with clinical and subclinical urinary duct infections is of great concern worldwide. The present cross-sectional study was conducted on 114 patients affected with urinary duct infections and admitted to Shahid Ghazi Hospital in the first half of 1393, on the aim of the bacteriological evaluation of urine samples and identification of antimicrobial resistance profile of the isolated bacteria. The youngest patient was 9 and the oldest one was 85 years old, and 96% were women and 4% were men. The relative frequency of bacteria isolated from the urine samples in bacterial culture were 74% Escherichia coli, 20% Staphylococcus spp., 3% Streptococcus spp., 2% Klebsiella spp., and 1% Pseudomonas spp. The antibiotic susceptibility profile was investigated by antibiogram test in accordance with NCCL protocol. The standard used antibiotic discs contained sulfamethoxazole, ceftizoxime, tetracycline, amikacin, cephalexin, nalidixic acid, gentamicin, nitrofurantoin, cefotaxime, ciprofloxacin, ampicillin, vancomycin, co-trimoxazole and norfloxacine. The most and the least susceptibility in Escherichia coli were for nitrofurantoin and sulfamethoxazole, respectively. Staphylococcus and Streptococcus isolates had the most resistance to ampicillin and sulfamethoxazole and the most susceptibility to nitrofurantoin and gentamicin. Klebsiella and Pseudomonas had the most antibiotic resistance for tetracycline and co-trimoxazole and the most susceptibility to gentamicin and amikacin, respectively.
Based on the importance of antibiotic resistance emerging and the alteration in antimicrobial resistance pattern of bacteria, antibiogram tests are essential for treatment of urinary tract infections.

Chronic Otitis Media (COM) remains one of the most common childhood chronic infectious diseases worldwide. This study was carried out to determine the organisms inducing COM and their drug resistance patterns in Zanjan. This cluster cross-sectional study was conducted on 100 patients suffering from COM in the age range of 18-45. COM specimens were obtained in operating room and then cultured on 4 different media. Following the diagnostic tests and identifying the bacteria, their susceptibility to antibiotics was assessed. The most common isolated bacteria were Pseudomonas aeruginosa (24.7%), Staph ylococcus aureus (17.6%) and Proteus spp (15.2%). The results of bioassay tests for isolated bacteria were as follows: Pseudomonas aeruginosa had the highest resistance to cefixime and co-trimoxazol (100%). The highest resistance of Staphylococcus Aureus was to co-trimoxazol (70%) and cefixime (50%). Regarding the multi-bacterial etiology of COM and high prevalence of antibiotic resistance, identification of causative bacteria and bioassay test prior to any therapeutic procedure seams necessary.