جستجوی مقالات مرتبط با کلیدواژه « تکثیر سلولی » در نشریات گروه « پزشکی »

In recent years, immunotherapy using chimeric antigen receptor T cells (CAR T cells) has been considered as a novel and promising treatment for some diseases, especially cancer. The CAR T cell production is a multi-step, complex, time-consuming, and costly process. One of the most important steps in production of CAR T cells is expansion of these cells at appropriate numbers for injection. Therefore, the aim of this study was to investigate the methods of expansion for human CAR T cells in ex vivo.

In this study, specifically engineered CAR T cells against the MUC1 tumor antigen were prepared. The cells were then treated with rIL-2, anti-CD3/C28 Dynadeads, and allogeneic PBMC (Rapid expansion protocol (REP)) for 14 days.

The results showed that using anti-CD3/CD28 antibodies in effective ratios; 1: 1 and 1: 3 (bead: cell) along with (IU100) rIL-2 resulted in a 27.5-fold increase in the number of cells. However, the use of rIL-2 alone or allogeneic PBMC eventually resulted in 4.5-fold increase and 9-fold increase, respectively.

Anti-CD3/CD28 in combination with rIL-2 can provide all three signals required for T cell activation and proliferation which can be a suitable alternative to costly and expensive cell proliferation techniques for animal or human CAR T cell studies.

Colorectal cancer is the third most common cancer in Iran. Some studies have shown that age and sex are associated with the course and prognosis of colorectal cancer. Due to the fact that recognizing the factors affecting colorectal cancer improves the screening system and selects better treatment modalities, and due to the lack of such studies in Iran, in this study, the relationship between the degree and stage of malignancy with environmental factors, gender and age of patients was examined for colorectal cancer.

This is a descriptive cross-sectional study and the pathology report of samples of patients with colorectal cancer admitted during 1393-1397 in Poursina Hospital in Rasht (Iran) in terms of age, sex, tumor location and pathological stage of patients undergoing was evaluated.

From 94 pathology reports, the number of men was more than women and the highest number of patients were reported in the age group of 50-60 years. Abdominal pain was the most common clinical symptom at diagnosis. Most tumors were located in the rectum. Stage III had the highest frequency. Higher stages were statistically significantly associated with being a woman. There was no significant relationship between age and smoking and alcohol consumption with colorectal tumor stage. Also, the degree of tumor was not related to age and sex and history of smoking and alcohol consumption of patients.

In this study, a significant relationship was found between the female gender and the higher stages of cancer. But there was no association between age and sex and smoking and alcohol consumption with the degree of cancer. Also, no relationship was found between age and smoking and alcohol consumption with the stage of cancer.

ncRNAs have been identified as oncogenic drivers and tumor suppressors in any type of cancer. Although many classes of ncRNAs have been reported, most studies have been performed on microRNAs (miRNAs). miRNAs can regulate several target genes and affect important processes such as homeostasis, angiogenesis, cell proliferation, differentiation, and apoptosis. Located in the p75NTR gene, miR-6165 is known to induce apoptosis in colorectal cell lines, and one study proposed a tumor suppressive role in colorectal cancer. However, its mechanism of action in breast cancer is not completely understood yet. Therefore, this study aimed to consider the expression level and the effect of miR-6165 on the proliferation and migration in breast cancer.

Fifty tumor and adjacent non-tumor tissues were examined in the study. miR-6165 levels were evaluated by qPCR in breast cancer cell lines and tumor tissues. Pre-mir-6165 was cloned in the pEGFPN1 vector. Next, human breast cancer MCF7 cells were cultured and pre-miR-6165 vector was transfected to breast cancer line. The effects on cell proliferation and migration were investigated with MTT assay and scratch test, respectively. Bioinformatics analysis was performed through enrichment and hub genes finding for miRNA targets.

miR-6165 was overexpressed in breast cancer tumor tissues and cell lines. High expression of miR-6165 was directly related to the metastasis. miR-6165 increased proliferation and migration in breast cancer cell line.

miR-6165 may function as an oncomir and increase the growth and migration of cells which may consequently serve as a therapeutic goal for breast cancer.

Breast cancer is the most common cancer among women. Studies have displayed that the dysregulation of microRNAs (miRNAs) plays a crucial role in the incidence, progress, and metastasis of tumors. miRNAs have been considered as a biomarker as well as a therapeutic agent, thus gene therapy with miRNA is considered as one of the ways to treat cancer. miR-4516 has been reported to play a role in different diseases and malignancies. However, the expression level and biological function of miR-4516 in breast cancer remain poorly understood. This study aimed to investigate the expression level and the effect of miR-4516 on the proliferation and migration in breast cancer.

Tumor and adjacent non-tumor tissues were collected from 40 patients with breast cancer. MiR-4516 levels in breast cancer cell lines and tumor tissue were measured by real-time PCR. Pre-mir-4516 was cloned in the pEGFPN1 vector. Then human breast cancer MCF7 cells were cultured and pre-miR-4516 vector and control vector were transfected into cells. After transfection, a miR-4516 level was verified by QPCR. Cell proliferation and migration were studied with MTT assay and scratch test respectively.

miR-4516 was down-regulated in breast cancer tissues and cell lines. Low expression of miR-4516 was directly related to the high-grade tumor. miR-4516 suppressed breast cancer cell proliferation, but not migration in vitro.

miR-4516 may function as a tumor suppressor and inhibits the proliferation which may consequently serve as a therapeutic target for breast cancer.

Research suggests the inhibitory effects of deferoxamine as an iron chelator on erythroleukemia cells. The present study was conducted to investigate the effects of deferoxamine on B92 cells as a model of glial cells carcinoma.

The present experimental study treated 6×104 B92 cells with 0, 10, 50 and 100 µM of deferoxamine for 24 hours in the presence or absence of 10 μmol/l of ferric chloride. Morphological changes were evaluated in the treated cells compared to in the control sample using an inverse optical microscope. The inhibitory and cytotoxic effects of deferoxamine were evaluated using the dimethylimidazole-diphenyl tetrazolium bromide (MTT) reduction and neutral red uptake assay. The data were analyzed using the Kruskal-Wallis test. P <0.05 was set as the level of statistical significance.

The inhibitory effects of deferoxamine on the proliferation of B92 cells were identified after 24 hours in a way that the cells began to accumulate in the presence of deferoxamine. Ten μmol/l of ferric chloride prevented these morphological changes. Deferoxamine was also found to significantly and 
dose-dependently inhibit the vitality and viability of B92 cells. Moreover, the data showed that ferric chloride prevents the emergence of the effects of treating B92 cells with deferoxamine.

Deferoxamine exerts in-vitro antiproliferative effects on the glial cell line B92.

Due to increase of infertile couples, potential differentiation and proliferation of umbilical cord mesenchymal stem cells (MSCs) and bone marrow stem cells (BM-MSCs) was compared to find proper stem cells for differentiation into germ-like cells. In this experimental study, isolated umbilical cord and bone marrow mesenchymal stem cells were treated by Retinoic acid (10-6M) and Sertoli cells condition medium. Viability percentage and the rate of proliferation (population doubling time) of cells was calculated in both groups. The number of colonies was evaluated in different days of culture, and finally the expression of and meiotic genes investigated by RT-PCR. The viability percentage was higher in BM-MSCs group and the rate of proliferation of cells increased by elevating the passage number. The number of colonies in the bone marrow stem cells was significantly higher than that of the umbilical cord MSCs (p<0.05). In contrast, the expression of PLZF, OCT4 and SCP3 genes were detected in umbilical cord MSCs after 10 days of culture. However, in BM-MSC, the expression of PLZF and SCP3 genes was observed only after 15 days of culture. It seems that the human umbilical MSCs higher differentiation potential for producing germ-like cells when compared to the Bone marrow stem cells. In contrast, the proliferation potential of BM-MSCs is greater than umbilical cord MSCs. This difference is probably due to secreted growth factors from these cells.

Considering the high prevalence of prostate cancer and the effect of androgens on its progression, this study was conducted to investigate the inhibitory effects of tamoxifen as an anti-androgen on prostate cancer. In this experimental study, the human cell line (PC3) was purchased from the Pasteur Institute. The effect of tamoxifen at concentrations of 0, 3.25, 7.5, 15, 30 and 60 μM on cells was evaluated, and the tests of viability, migration, colonization and cell morphological changes were respectively performed using MTT, wound healing, colonization, and giemsa staining methods.
FINDINGS: IC50 dosage of tamoxifen of 15 μM with a regression coefficient of 0.90 was obtained within 24 hours. The results showed that tamoxifen significantly inhibited proliferation with 7.3±0.6 colonies compared with 100 colonies of control (p<0.03) and migration with 278.4±1.5 μm groove diameter compared with 89.68 ± 0.9 μm of control (p<0.01) at the dose of 15 μM. Treatment of cells with a dose of 15 μM also causes changes in the nucleus and cytoplasm and causes apoptosis in comparison with the control group. The results of this study showed that tamoxifen has significant inhibitory effects on PC3 prostate cell and can be considered as an appropriate way for the treatment of prostate cancer.

Using natural compounds with low toxicity on normal cells and high efficacy on malignant cells is highly appreciated for treatment of colorectal cancer (CRC). In the present study, the effect of fish-oil derived eicosapentaenoic acid (EPA) on the cell number, cell proliferation rate and caspase-3 enzyme activity in LS174T human colorectal cancer cell line was investigated.
This experimental study was performed in cell culture lab, Institute of Biotechnology, affiliated to the Urmia University, Urmia, Iran from April to September 2017. LS174T colorectal cancer cells at a density of 5×105 cells per well were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) and kept at 37 °C in a humidified incubator with 5% CO2 for 24 hours. Thereafter, the cells were treated with 50, 100, 150 and 200 μmol EPA for 48 hours and cell numbers were counted using neobauer chamber and caspase-3 activities were measured by performing the caspase-3 colorimetric assay (Abcam, Cambridge, MA, USA). Furthermore, 5×103 LS174T colorectal cancer cells were cultured and treated with the above-mentioned EPA concentrations for 24, 48 and 72 hours, after which cell proliferation rate was evaluated by WST-1 proliferation assay (Roche Diagnostics, Mannheim, Germany).
Treatment of LS174T colorectal cancer cells with 50, 100, 150 and 200 μmol EPA decreased the number of cells in a dose-dependent manner. We also found that treatment of malignant cells with increasing EPA concentrations (50 to 200 μmol) significantly decreased cell proliferation in a dose and time dependent manner. After a 72 hours treatment of LS174T cells with 200 μmol EPA, cell proliferation was calculated to be 30.3% compared to untreated control cells. Following 48 hours treatment, caspase-3 activity increased with increasing EPA concentrations in which at 200 μmol EPA, caspase-3 activity increased by 3.4 fold compared to untreated control cells.
Fish-oil derived eicosapentaenoic acid as a safe compound decreases the number of colorectal cancer cells and their proliferation rate and activates caspase-3 enzyme, as an executor protein in apoptosis

Long non-coding RNAs (LncRNAs) are a class of RNA molecules with 200-10000 nucleotides in length that have lost their protein coding capacity. They play important role in numerous biological processes including cell proliferation, differentiation, apoptosis, and cancer development. Acute myeloid leukemia (AML) is the most common leukemia. Evidence has shown that long non-coding RNAs PVT1 is upregulated in acute myeloid leukemia, and leads to an increase in cell proliferation. This study aimed to assess the effect of long non-coding RNA PVT1 inhibition by antisense LNA GapmeRs technology on proliferation of human acute erythroleukemia cells.
Human acute erythroleukemia cells (KG1), known as subgroup six of acute myeloid leukemia based on the French–American–British (FAB) classification, were cultured in Roswell Park Memorial Institute (RPMI) medium. Long non-coding RNA PVT1 degradation was performed using antisense LNA GapmeRs technology. At different time points after transfection (24, 48, and 72 hours), long non-coding RNA PVT1 expression and cell proliferation were assessed. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was accomplished to evaluate the long non-coding RNAPVT1 expression. Moreover, Cell viability was measured using 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolium bromide (MTT) assay.
Findings: Long non-coding RNA PVT1 expression decreased at 24, 48, and 72 hours after transfection in the antisense LNA GapmeRs group compared to control groups. Cell viability and proliferation was significantly decreased in antisense LNA GapmeRs transfected group compared with other two groups.
The present survey exhibited that inhibition of long non-coding PVT1 by using antisense LNA GapmeRs can dramatically reduce the proliferation of human acute erythroleukemia cells. Our results can be helpful in translational medicine for antisense therapy in acute myeloid leukemia.

In order to enhance in vivo stem cell viability and considering similar anti-inflammatory and anti-apoptotic effects of human adipose derived stem cells (hADSCs) and astaxanthin (AST) and their ability to cross the blood-brain barrier, we investigated the effects of (AST) on hADSCs proliferation and viability to provide a supplement for cell based therapy in MS patients.
After isolation of hADSCs and assessment of CD markers, they were cultured in DMEM-F12 medium in the presence of AST at various concentrations (1, 5, and 10 ng/ml) for 72 h. Finally, we assessed cell proliferation and cell viability by MTT and Tryphan Blue methods.
The results revealed that a high percentage of hADSCs expressed CD90 and CD44 markers and a low percentage of them expressed hematopoietic cell markers. In addition, in the group cultured in the presence of 5 ng/ml AST the mean percentage of cell viability increased significantly compared to other groups (p = 0.04). Tryphan Blue results also revealed significant effect of AST on stem cell proliferation and culture of these cells in the presence of 5 ng/ml of AST, led to significant increase in the mean percentage of cell count compared to the results of the control group (p = 0.03).
Astaxanthin can increase hADSCs proliferation and survival and this agent can be used in the cell-based therapies in MS patients.

Chondroitin sulfates (CS) is an important glycosaminoglycan’s which is found in sturgeon fishes which are present in cartilage of these fishes. This compounds, in addition to application in food and cosmetic industries, they have clinical significant in arthritis treatment and wound healing.
In present study, the chondroitin sulfate extraction was performed by using pepsin and trypsin digestions with 12 and 18 h interval times followed by cationic salt, cetylprydinium chloride (CPC) treatment. MTT assay at different incubation time (24, 48 and 72 h) was used in order to study of Chondroitin sulfate effect on induction of proliferation of fibroblasts isolated from human skin. In order to determine the proper time and enzymatic digestion, after temperature treatment of samples using pepsin and trypsin enzymes, the SDS-PAGE electrophoresis was used. And the FT-IR analysis was performed to characterize the type of CS.
The maximum yield of enzymatic digestion was observed for trypsin digestion at 18h, according to electrophoresis patterns. the amount of CS yield was estimated at 4.76% in this situation. FT-IR analysis revealed that, the CS belongs to the C4S type. Chondroitin sulfates extracting from cartilage showed concentration-dependent incremental impact on fibroblast cell proliferation so that the dose-response results have shown 167% proliferation at 10 µg/ml dose of CS compared to the control in fibroblast cells.
Extracted CS has a positive stimulatory effect on fibroblast cells proliferation in a dose dependent manner. So it can be used for wound healing and some fibroblast related diseases.

All natural anticancer agents are cytotoxic basically and act mainly by the inhibition cell proliferation; but they have different mechanisms. Two assays, thiazolyl blue [3- (4, 5-dimethylthiazol-2-yl) -2, 5-diphenyl-terazoliumbromide or MTT] and sulforhodamine B (SRB), are used to assess cell growth. This study aimed to compare measurements between MTT and SRB on the cancer cell lines.
Different concentrations of the bromelain were added to cultured cells including mouse breast cancer (4T1), human gastric carcinoma (AGS), and human prostate carcinoma (PC3) cell lines and incubated at 24 and 48 hours. The growth and proliferation rates of the studied cells were investigated using both MTT and SRB assays after treatment with bromelain. The differences between cells were determined using Kruskal-Wallis and Dunns tests.
Findings: Bromelain significantly decreased growth and proliferation rate of 4T1, AGS and PC3 cancer cells, in a concentration-dependent manner at different times, in both MTT and SRB assays.
Findings showed that both MTT and SRB assays gained similar data regardless of the cell types.

Background and The use of an appropriate stimulus for increasing the rate of neural stem cell proliferation is one of the most important issues in cell therapy. This study aimed to investigate the effect of hydroethanolic extract of Stachys byzantina on hippocampus-derived neural stem cell proliferation and apoptosis.
In this experimental study, the neural stem cells were isolated from the hippocampus region of brain using enzymatic digestion. The neurospheres were dissociated to single cells and cultured on adherent plates. For these cells, immunocytochemical evaluation was performed for marker nestin. The isolated neural stem cells were pretreated with different doses of hydroethanolic extract of Stachys byzantina for 48 h, and then exposed to 125 μM of H2O2 for 30 min. The effects of this extract on cell survival and apoptosis were evaluated using MTT and TUNEL, respectively. To analyze the data, ANOVA and Tukey’s tests were run in SPSS, version 15.
In the current study, 800 µg/ml of Stachys byzantina extract significantly increased the proliferation rate of the neural stem cells. Furthermore, the results of the TUNEL staining demonstrated that Stachys byzantina extract did not have any protective effect on hydrogen peroxide-induced apoptosis.
Stachys byzantina extract increases the rate of neural stem cell proliferation. However, further studies are required to determine the effect of this extract on the treatment of neurodegenerative diseases.

Nowadays, investigation of the biological effects of mobile phones is important due to increasing use of these devices. This study aimed to assess the effects of exposure with 900-MHz mobile phone radiation on survival and proliferation of breast cancer Michigan cancer foundation-7 (MCF-7) cells.
Breast cancer MCF-7 cells was used and divided into 3 radiation groups which located at 10 and 20 cm from Global System Mobile Communication (GSM) antenna signal simulator that is able to produce electromagnetic waves with a frequency of 900 MHz. The cells were exposed 6, 15 and 30 minutes per day with an interval of 10 minutes during exposure time for 3 and 5 days, respectively. After 3 and 5 days, MTT assay [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] was used to evaluate the growth and viability. Trypan blue test was done to assess the rate of cells proliferation. Data analysis was performed using SPSS software.
Findings: Irradiation times and periods of 3 and 5 days had a significant impact on viability of MCF-7 cells (P 0.05).
The results showed that 900-MHz radio-frequency electromagnetic fields (RF-EMF) signal radiation may reduce cell viability and proliferation rates of the MCF-7 cells regarding to the duration of exposure.

Cancers are among the serious problems in communities. In recent years, herbal products have been very promising regarding their anti-tumor and immunomodulatin potentials. SIM5, a herbal preparation, has had good results in discriminating between cancerous cell lines and normal cells, but have not examined directly on patients cells yet. In this study, bone marrow mononuclear cells from cancerous and non-cancerous samples were used to study the effects of SIM5.
Remained bone marrow samples of multiple myeloma patients (7 cases) and individuals who clinically needed bone marrow examination, but finally were diagnosed with no serious pathologic conditions in their leukocytes (7 cases) were considered as control group. Mononuclear cells were isolated and cultured with SIM5 and R10 fraction. After 48 h incubation, toxicity was assessed using LDH release test and vital activity with MTT test.
Cell toxicity with SIM5 was observed only in multiple myeloma samples and not in control group samples. There was a strong correlation between toxicity and the number of plasma cells in bone marrow mononuclear cells. SIM5 induced a decrease in vital activity of cancerous cells but an increase in the normal bone marrow samples.
SIM5 has distinct effects on normal and cancerous cells, and its toxic effect is focused on the latter. Therefore, it possesses the properties of an ideal product to be studied more seriously.

Astrocytes are the most abundant cells in the mammalian brain and play important roles in regulating neuronal signaling, protecting neurons, and determining the fate of neural precursors. Astrocytes are one of the best candidates for cell therapy. Three-dimensional (3D) scaffolds are a synthetic matrix which provided a biocompatible, biodegradable, and non-toxic 3D environment for a variety of cells. In the present study, we isolated astrocytes from rat neocortex and assessed the proliferation rate of astrocytes in 3D dimensional environment.
Astrocytes isolated from adult rat neocortex. The isolated cells was cultured in DMEM/F12 medium supplement with 10% FBS. Astrocytes were confirmed by expression of GFAP (glial fibrillary acidic protein), assessed by immunocytochemistry. After the 3th passage, the astrocytes were cultured in 3D and two - dimensional (2D) cultures and MTS assay was used to determined cell proliferation.
The expression of GFAP marker was confirmed by immunocytochemistry assay. Our results showed that surface plating of astrocytes with 0.15% Pura Matrix showed significantly greater cell proliferation in comparison with 2D culture.
These findings indicate the potential of the astrocytes which was cultured in 3D for using in cell transplantation.

Nowadays, extensive research is carried out on the effect of plant extracts on variety of disorders. In this study, the effect of hydroethanolic extract of Lavandula officinalis on proliferation of neural stem cells as well as its anti-apoptotic properties was assessed.
Neural stem cells were isolated from hippocampus of neonatal rat brain. To determine the optimal concentration of Lavandula officinalis extract, isolated neural stem cells were treated with 200, 400, 600, 800 and 1000 µg/ml of concentrate for 48 h. Then cell proliferation rate was evaluated via MTT assay. Meanwhile, the anti-apoptotic property of Lavandula officinalis extract was evaluated using TUNEL assay method.
The results of this study showed a significant difference in cell proliferative and anti-apoptotic property of Lavandula officinalis extract on neural stem cells comparing to the control group.
The hydroethanolic extract of Lavandula officinalis can be effective on proliferation elevation and apoptosis reduction in rat neural stem cells.

Hepatocellular carcinoma remains often refractory to classic therapies. Therefore, the search for new natural compounds with minimal toxicity is of particular interest in treatment of liver cancer. In this context, it has been shown that 2-methyl-3-pentyl-6-methoxyprodiginine isolated from cell wall of Serratia marcescens has powerful growth inhibitory effects against different kinds of cancer cells. In this study, we investigated the anti-proliferative effect of prodigiosin on the human hepatocellular carcinoma HepG2 cells on a dose-response and time-course basis.
Malignant cells were treated with various concentrations of prodigiosin (100, 200, 400 and 600 nM) and incubated for 24 to 72 h. After treatments, proliferation-rates were determined by MTT assay. Following 48 h treatments with indicated concentrations of prodigiosin, cell number and apoptotic rates were also measured by hemocytometer and flow cytometer respectively.
Treatment of cells with increasing concentrations of prodigiosin significantly decreased proliferation-rates in a dose-and time-dependent manner compared to untreated cells. Specifically, Following 72 h treatments with 100, 200, 400 and 600 nM prodigiosin proliferation-rates were found to be %44 ± %2, %33 ± %4, %27 ± %3 and 27% ± %5 for indicated concentrations of prodigiosin respectively. We also found that the cell numbers were decreased in a dose-dependent manner. Specifically, 48 h treatment with 600 nM prodigiosin resulted in 77% decrease in cell number compared to untreated cells. An increase in the number of apoptotic cells (late), ranging from 37 to 97.4 %, was also observed with increasing prodigiosin concentrations.
Prodigiosin decreases proliferation of hepatocellular carcinoma cells with induction of apoptosis in these cells. Therefore, this compound may provide a powerful growth inhibitory agent on proliferation of hepatocellular carcinoma cells.

Mesenchymal stem cells (MSCs) contribute to tissue repair in vivo and form an attractive cell source for tissue engineering. The regenerative potential of MSCs is impaired by oxidative stress-induced cellular senescence. Alpha-lipoic acid (ALA) is well-known for its antioxidant properties. The Ki-67 antigen is expressed during all phases of cell cycle (G1, S, G2 and M phase) except for G0 phase and is commonly used as a proliferation marker. Herein, the aim of the present study was to investigate the impact of ALA on rat MSCs survival and proliferative potential in vitro.
Isolated rat bone marrow and derived mesenchymal stem cells were synchronized by serum starvation for 24h and the addition of hydroxyurea (2µM). Afterwards, the cells were cultured in the presence of ALA (1µM) for 48h. An MTT assay was used to investigate cell survival and proliferation. The expression of Ki-67, a proliferation marker, was also evaluated.
The MMT assay showed a statistically significant increase in proliferation of MSCs in ALA-treated groups for 48 hours. Immunoctytochemistry of Ki-67 revealed significant differences between ALA- treated and Control groups.
In conclusion, ALA is effective in increasing the survival and cell proliferation of isolated rat bone marrow and derived mesenchymal stem cells.