جستجوی مقالات مرتبط با کلیدواژه « رده سلولی K562 » در نشریات گروه « پزشکی »

Chronic myeloid leukemia is one of the most well-known types of leukemia. Inflammation is one of the leading causes of cancer; therefore, anti-inflammatory agents are used for reducing and suppressing the growth of cancer cells. Dexamethasone, a cortisol agonist, has anti-inflammatory, anti-tumor, and apoptotic effects. Diclofenac is a cyclooxygenase enzyme inhibitor with anti-inflammatory properties. This study was performed to determine the synergistic effect of diclofenac and dexamethasone on the growth of K562 cancer cells.

In this descriptive-analytical study, K562 cell line was cultured in RPMI-1640 medium enriched with glutamine, penicillin, and streptomycin. The cytotoxic effects of dexamethasone, diclofenac and their combination (multi-target tracking) were evaluated using MTT assay. Hoechst staining and DNA electrophoresis were carried out to evaluate the occurrence of apoptosis.

Diclofenac, dexamethasone and their combination had cytotoxic effects on the cells at concentrations of 20, 40, 60, and 80 µmol/ml. A significant cytotoxic effect was observed after 72 hours of treatment with different concentrations of the drugs (P<0.05). Hoechst staining showed that DNA fragmentation was increased in the treated cells. DNA electrophoresis also showed induction of apoptosis by diclofenac, dexamethasone, and their combination.

The combination of diclofenac and dexamethasone at concentration of 20 µmol/ml is more effective in inducing apoptosis in K562 cells compared with each drug alone.

Nowadays, more than 100 types of cancer are known worldwide, which leukemia is one of the most common type. Chronic myeloid leukemia (CML) is one of the most recognized forms of leukemia, accounting for between 15% and 20% of total leukemia. Efforts to find new synthetic compounds are one of the basic strategies for finding new drugs for CML treatment. The purpose of this study was to investigate the effects of growth suppression and induction of apoptosis (family of pyrazinoic acid) on the cancer cell line of K562 leukemia. In this study, the effect of a certain concentration of 2-oxo-1, 2-diphenylethyl-pirazine-2-carboxylate (2 DP.P) was evaluated on the growth, cell cycle and induction of apoptosis of K562 cells using various techniques. MTT test was used to investigate the effects of 2-DP.P on cell viability. For this purpose, 5×104 of K562 cells were cultured at concentrations of 25-100 μM from the 2-DP.P for 24, 48 and 72 hours intervals. Electrophoresis, fluorescence microscopy and flow cytometry were used for analysis of apoptosis. The 2-DP.P inhibited dose and time dependently growth in K562 cells. IC50 of the compound was calculated 25 μM. Also, data from fluorescence microscopy, DNA fragmentation and cell cycle analysis confirmed the reduction of viability and induction of apoptosis by the compound. due to the effects of growth inhibition and induction of apoptosis by the 2-DP.P, this compound can be suggested as a new and effective compound for further studies in treating patients with leukemia.

Probiotics are defined as different microorganisms that may have positive effects on preventing or treatment of special pathologic conditions. Anti-cancer properties of these bacteria have been shown in few studies. The aim of this study was to evaluate anti-tumor effects of heat-killed L. casei and L. paracasei on K562 cell line (chronic myeloid leukemia). In this experimental study, bacteria were cultured in special medium at anaerobic condition, then, cells killed at temperature of 100°C. Killed cells were dried by lyophilization, finally were sterilized by autoclaving. Then, different concentrations of heat-killed bacteria (125, 250, 500, 1000 and 2000 μg/ml) were prepared separately. Anti-tumor properties of heat-killed bacteria were determined in vitro at 24, 48 and 72 h with MTT assay. Our result showed that heat-killed cells of L. casei and L. paracasei statistically (p˂0.05) have Anti-tumor properties in K562. In addition, Anti-tumor properties of heat-killed cells have direct relationship with concentration. Based on obtained results we conclude that the heat killed Lactobacillus can be used as a potential candidate for further studies on chronic myeloid leukemia treatmentces.

The level of NF-κB factor expression (a transcriptional factor which increases the expression of inflammatory genes) is often increased in various human cancers. Therefore, NF-κB inhibitors such as Celastrol may prevent cancer development. The purpose of this study was to evaluate the anticancer effects of Celastrol on K562 cells proliferation. First, the K562 cells were cultured and cytotoxicity effects of celastrol were determined by MTT assay. Hoechst staining and DNA electrophoresis are used to check apoptosis. Data analysis was performed using SPSS, version 16 and ANOVA test (P<0.05) Statistical analysis of MTT assay data showed that the growth of treated cells with different concentrations of the Celastrol significantly decreased (P<0.05) and inhibitory effect of Celastrol was time and concentration–dependent; so in higher concentrations (8 µM) and 72 hours, the maximum effect has occurred. The IC50 value of Celastrol was obtained 4 µM. Also, the results of Hoechst staining and DNA electrophoresis showed that Celastrol caused fragmentation of cell nucleus and DNA. Based on the results, Celastrol decreases cells viability (P<0.05) and induces apoptosis in K562 cells, its effect is time and dose-dependent. In conclusion, the agent may be applied as an anticancer drug for treatment of chronic myeloid leukemia.

Cancer is the second leading cause of death in the world and among the types of cancer, leukemia is one of the deadliest. Chronic myeloid leukemia (CML) is the best known types of leukemia. Hydralazine is in the cardiovascular medical group, which is considering as inhibitory factor on the proliferation of cancer cells by knowing its inhibitory effects on DNA methyltransferase. The purpose of this study was to evaluate the inhibitory effects of hydralazine on K562 cells proliferation.
First, the K562 cells were cultured. After, the different concentrations of the hydralazine were prepared and the cells were treated for 24, 48 and 72 hours. Next, the inhibitory effects of this drug on K562 cells proliferation was measured by MTT assay. Hoechst (33342) staining and DNA electrophoresis was used to check apoptosis. Data analyses were performed using SPSS software (Version 20) and ANOVA test (P MTT assay results showed that hydralazine has the highest cytotoxic activity in 80μM at 72 hours. Drug IC50 was obtained 81 micromolar. And also the results of Hoechst staining and DNA electrophoresis showed that hydralazine was caused apoptosis.
In the present study, it was shown that hydralazine induces cells death by apoptosis and this effect was dose-dependent and treat time dependent. It is suggested that more studies will be done on the effects of this drug on animal models and human.

Lovastatin is a HMG-CoA reductase inhibitor and used for the treatment of hypercholesterolemia. Inhibition of HMG-CoA reductase results in inhibiting the activity of the Ras proto-oncogene that has mutations in most cancers. This study was done to determine the Anti-proliferative and apoptotic effects of Lovastatin on K562 Erthromyloidy cancer cell line.
The K562 Erthromyloidy cancer cell line were cultured and treated with different concentrations of lovastatin. Their antitumor effect on K562 cells were assessed via MTT assay after 72 hours. Hoechst (33342) staining and DNA electrophoresis were used for study of apoptosis.
Lovastatin had antitumor effect on K562 Erthromyloidy cancer cell line and this effect increased by incease of time and concentration.The maximum inhibitory effect was 59% in higher concentration (100 µM) and 72 hours after the treatment. Reduced cell growth at 24 and 48 hours after treatment was 24% and 43%, respectively. Lovastatin significantly inhibited K562 cell growth (P This study showed that lovastatin has antitumor effect on K562 Erthromyloidy cancer cell line.

Fetal hemoglobin is the predominant hemoglobin expressed by gamma globin. However, in adults, fetal hemoglobin normally reduces to very low levels of the total hemoglobin. The increase in levels of fetal hemoglobin can ameliorate the severity of β-hemoglobin disorders such as sickle cell disease and beta-thalassemia. Currently, drugs that have been used for induction of Fetal hemoglobin (HbF) have short-term effects. Dendrosomal nano-curcumin (DNC), which has high solubility and absorption, is able to detect different targets in the cell and affect gene expression. LSD1 is one of the most important gamma globin inhibitors. In this study, we examine the capability of DNC to inhibit the expression of LSD1, GATA1, and FOG1 as well as the increase in gamma globin expression.
We used the K562 cell line for the MTT assay and treatment by DNC. Then the effect of DNC on the increase in expression level of γ-globin, decrease expression level of LSD1 and transcription factors, GATA1 and FOG1was investigated by Real time PCR.
Data acquired from gene expression assays indicated that DNC induced gamma globin expression and decreased expressions of LSD1, GATA1, and FOG1 in a time and dose-dependent manner.
Inhibition of LSD1, GATA1, and FOG1 expressions via DNC led to increased gamma globin expression. These results showed that DNC could be a promising treatment for beta-thalassemia and sickle cell disorders, and possibly reduce the severity of symptoms of these patients through the induction of fetal hemoglobin.

Background and aim of study: Leukemia is cancer of blood-forming tissues which derives from red and white blood progenitors cells. According to the needs of body, Red and white blood cells usually grow, and divide precisely, and orderly. But in the leukemia, the regulation is disrupted, and it makes the growth of blood cells control aside. Nitroglycerin by transferring to nitric oxide (NO), and increasing of NO cellular oxidative stress cause induces apoptosis of cancer cells. This study was performed to survey the antitumor effects of nitroglycerin drug on K562 cells in vitro. First, The leukemia cells K562 category are cultured in RPMI 1640 with 10 percent of heat-inactivated fetal calf serum, then different concentrations of drug prepared, and their antitumor properties at 24, 48 and 72 hours after treatment was measured by MTT method. Then the drugs IC50 measured. In the next stage, in order to survey the apoptotic, electrophoresis and staining with Hoechst colors used. The results showed that the antitumor impacts of nitroglycerin dependently increase in a dose, and time. The IC50 of nitroglycerin was observed at 79 (µmol /ml).Also, the results of electrophoresis and staining showed that these drugs have the effect of apoptosis. Based on the results, can be inferred that inhibitory effect of nitroglycerin on K562 cells depends on time, and dose which the maximum inhibitory effect observed after 72 hours of treatments of the highest concentration. So, this drug may be effective in the prevention, and treatment of chronic leukemia.

Probiotics are living microorganisms that have beneficial effects، such as anti-tumor effects on the health. The aim of this study was to evaluate the inhibitory effect of cell wall and cytoplasmic extracts of Saccharomyces cerevisiae as a probiotic cancer Category K562 (myeloid leukemia) and apoptotic effects of this yeast. In this experimental study، the Saccharomyces cerevisiae was cultured، and using the micro sonicator the cell wall and cytoplasmic extracts were separated. Then، different concentrations of cell wall and cytoplasmic extracts were prepared. The inhibitory growth percentage of cancer K562 cell line at different times (12، 24، 48 and 72 h) was measured by MTT assay. Apoptosis and necrosis were also evaluated by DNA fragmentation electrophoresis method. The collected dara were analyzed by ANOVA and Tukey test. Anti-tumor activity of cytoplasmic extracts significantly increased over time، while the impact of anti-tumor activity of the cell wall over time was significantly reduced (p <0. 05). The composition of the Saccharomyces cerevisiae also induces apoptosis، and less necrosis at K562 cell line. This study showed that the cell wall and cytoplasmic extracts of Saccharomyces cerevisiae in a time-dependent had an inhibitory effect on K562 cells and induced necrosis and apoptosis.