جستجوی مقالات مرتبط با کلیدواژه « سلول های جورکت » در نشریات گروه « پزشکی »

Acute lymphoblastic leukemia is a malignant hematologic disorder of lymphoid lineage. As a result of chemotherapy side effects, treatment with medicinal plants has recently been considered by researchers. Previous studies on Moringa olifera have shown its anti-cancer properties on some cancer cell lines. The aim of this study was to investigate the cytotoxic effects of Moringa oleifera leaves on Jurket and Raji cell lines of acute lymphoblastic leukemia.

In this experimental study, after preparing aqueous and ethanolic leaf extract of Moringa olifera, Jurket and Raji cells were treated with different concentrations of extracts for 48 hours. Cell groups’viability was assessed by trypan blue staining and MTT assay. Peripheral blood mononuclear cells (PBMC) were used as a control group. Statistical analysis of data was performed using Gragh Pad Prism 6 and one-way ANOVA test.

Treatment of Jurkat and Raji cells with all concentrations of aqueous and ethanolic extracts led to inhibition of cell growth. The highest percentage of growth inhibition was obtained at a concentration of 150 μg/mL of aqueous extract, 48.5% and 47.4% for Jurkat and Raji cell lines, respectively. Also, the highest lethal percentage was obtained at a concentration of 50 μg/mL of ethanolic extract, 73.4% and 78.5% for Jurkat and Raji cell lines, respectively. The IC50 dose was obtained in all study groups at a concentration of 150 μg/mL.

The leaves of Moringa olifera have the capacity to inhibit the growth and viability of Jurkat and Raji cell lines of acute lymphoblastic leukemia.

Recently, several long non-coding RNAs (lncRNAs) have been identified as being cancer-specific, namely metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), HOTAIR, and ANRIL. Studies have revealed that MALAT1, is up-regulated in many solid tumors and it have important regulatory roles in cancer biology, metastasis and recurrence. Acute myeloid leukemia (AML) and Acute lymphoblastic leukemia (ALL) are cancers of the myeloid and lymphoid blood cells lines, characterized by the rapid growth of abnormal white blood cells that accumulate in the bone marrow and interfere with the production of normal blood cells. KG1 and Jurkat cell lines cultured in IMDM and RPMI-1640 mediums respectively. Total RNA extracted from the cell lines and normal bone marrow cells and MALAT1 gene expression was measured by qRT-PCR. MALAT1 gene expression was significantly increased in the KG1cell line (P <0.05), while decreased in Jurkat cell line in compare to normal bone marrow cells (P <0.05). Our findings suggest that MALAT1 may function as novel biomarker for diagnosis, prognosis, metastasis, and predicting the response to therapy for the aforementioned types of cancers.