جستجوی مقالات مرتبط با کلیدواژه « هوموسیستئین » در نشریات گروه « پزشکی »

Some studies suggest that increased homocystein in blood leads to alterations in coagulation; however, the precise mechanism is not clear. The aim of this study was to compare different concentrations of homocysteine and aspirin on coagulation parameters and permeability coefficient of clot in the plasma of healthy individuals in vitro.

Different concentrations of homocysteine (50, 100, 200 µM) and aspirin (1, 10, 100 mg/l) were added to the plasma citrate. They were incubated at 37°C for 24 hours. Then, coagulation parameters were analyzed by the turbidimetric procedure at 405 nm and permeability coefficient of clot was determined. The independent samples t-test was utilized to compare the results.

Homocysteine at 200 µM with aspirin 100 mg/l had significant changes in the total coagulation time (820.00 ± 1.15 s), maximum coagulation velocity (0.232 ± 0.003), the permeability coefficient of clot [(1.108 × 10-6) ± 0.151] compared to homocysteine at 200 µM [555.00 ± 12.89, 0.258 ± 0.008, (0.493 × 10-6) ± 0.038, respectively] (P < 0.05). Homocysteine at 200 µM with aspirin 1 mg/l did not significantly change in either parameter (P > 0.05). Homocysteine at 100 µM with aspirin 1 mg/l significantly changed only in the permeability coefficient [(0.787 × 10-6) ± 0.071] compared to homocysteine at 100 µM [(0.636 × 10-6) ± 0.053] (P < 0.05).

Aspirin with the dose more than 1 mg/l had more effect on higher concentrations of homocysteine and delayed forming clot by impeding the coagulation. It increased clot permeability, too.

Background and

Ânalysis of cardiovascular diseases etiology and risk factors have pointed out strong correlation between cardiovascular disease and risk factors such as hypertension, diabetes mellitus, serum cholestrol level, although only 50% of coronary arteries diseases(ÇÂDs) are demonstrable by such factors. For this reason researchers have conducted many studies for definition of different risk factors and have proposed new markers such as homocystein.Such researchs on the basis of correlation between homocystein and ÇÂDs have showed different or even paradoxical conclusion. Ïn this research we have studied the associations of plasma homocystein levels with mortality and complications of Myocardial Ïnfarction with ST segment Ëlevation (STËMÏ) in patients admitted Fatemeh Zahra hospital of Sari, during the year 1389.

This study was Çross-sectional and we investigated the patients with STËMÏ who had no history of previous MÏ and had referred to hospital in less than 24 h from symptom beginning. 5 cc of blood sample was taken from all patients after 12 hrs fasting for detection of homocystein. Plasma lipids and suger were also measured. Patients were followed during hospitalization for mortality and electrical or mechanical complications then were compared in two groups of normal and increased homocystein.

Ôf 230 subjects, 150(65.2%) of patients had normal homocystein levels and others (80 or34.8%) had increased homocystein (>17micromol/lit). 2(1.3%)patients amonge subjects with normal homocystein levels,and 4(5%) patients amonge groupe with increased homocystein died. Âll of the mechanical complications (including; 2 (2.5%) VSD and 1(1.3%) tamponad) were detected in patients with increased homocystein. We had no case of MR. Finally, electrical complications were in 40(26.7%) patients with normal homocystein, and 19(23.8%) patients with increased homocystein. Ëxcept combination of tamponad and VSD in subjects with high homocystein, other complications including mortality, and electrical complications were no different in two groups of patients. Çonclusion: This study showed increased incidence of combination of VSD and tamponad in patients with high homocystein during STËMÏ. Ït not showed correlation between homocystein, with another mechanichal complications, electrical complications and hospital mortality of STËMÏ. This results support the modest correlation between homocystein and complications of STËMÏ.

Intorduction: Despite of success in treatment of cardiovascular diseases by drugs and other methods, cardiovascular diseases and myocardial infarction are the most common causes of death in the developing countries. Many reports have been published about correlation between free radicals and increase of homocysteine with atherosclerosis pathogenesis. The aim of this research was to study the correlation between the heaelevation of homocysteine with changes of superoxide dismutase (GPx) activity after myocardial infarction. Matrials and

This research was a descriptive cross-sectional study that performed in 2006. In fasting condition, blood was taken from 48 patients and 48 normal healthy people under 76 years old. Serum homocysteine was determined by ELISA method, erythrocyte GPx (rGPx) activity assayed by Randox company kit and serum GPX (sGPx) activity assayed by Cayman chemical company kit.

36 men and 12 women were in the patien group, while the control group included 29 men and 19 women. The mean of homocysteine in patients were 30.3 ± 5.3 µmol/L and in controls were 11.1 ± 3.1 µmol/L, that it showed a significant difference between two groups (p<0.001). The mean activity of sGPx in patients and controls were 2705 ± 2078 U/g Hb and 2509 ± 2450 U/g Hb, respectively. The mean activity of rGPx in patients and controls were 47.8 ± 25.6 U/ml, 44.8 ± 24.6 U/ml, respectively.

In this study, an increase in homocysteine, sGPx and rGPx was observed in patients in compared with controls; however the increase of GPX was not significant. It seems that the elevation of GPX is due to cellular damages. This enzyme inhibits free radicals.