جستجوی مقالات مرتبط با کلیدواژه « پارامترهای مایع منی » در نشریات گروه « پزشکی »

Infertility is a major problem of modern medicine as it affects almost 20% of reproductive-aged couples. The cause of this problem is attributed to the male partner in nearly 40%-50% of these cases. The role of herpes simplex virus (HSV) in male infertility has been investigated using the sensitive methods. The aim of this study was to determine the prevalence of HSV-1, 2 DNA in the semen, blood and urine of idiopathic infertile men and its association with altered semen parameters.
A total of 150 semen, blood and urine samples from infertile men were collected in the Shariati hospital, Tehran (2012-2014). Sample analysis and diagnostic PCR using specific primers was performed for detection of HSV-1, 2 DNA in the specimens.
Analysis showed six groups of infertile men, including HSV-1, 2 positive and negative groups in semen, blood and urine samples. HSV-1, 2 DNA was detected in 38 (18.66%) semens, (6.66%) 10 in blood and 2 (1.33%) in urine. Only HSV-1, 2 positive samples of semen had abnormal semen parameters.
Using a powerful molecular method, we detected a high prevalence of HSV-1, 2 DNA in the semen of asymptomatic infertile patients. Although HSV-1, 2 infections were not associated with motility and morphology defects of the sperms, it was related with decreased sperm count in the semen fluid. In addition there was not a significant role for detection of HSV-1, 2 DNA in blood and urine samples of infertile men and abnormal semen parameters.

Varicocele occurs in approximately 15% to 20% of the male population and it is the most common cause of poor semen. It has been demonstrated that patients with varicocele have poor chromatin packing, DNA damage and nuclear anomalies than the healthy men. Therefore, the aim of this study was to evaluate sperm chromatin integrity and semen parameter in patients after nonmicrosurgical subinguinal varicocelectomy. This study evaluated semen parameters and sperm DNA integrity before and 4 month after nonmicrosurgical subinguinal varicocelectomy from 30 men with varicocele in center of infertility in Ahvaz Jundishapour University of Medical Sciences. Parameters of semen were evaluated with WHO criteria and sperm DNA integrity was analysed by aniline blue and toluidine blue. The slides were checked by light microscopy and to determine the percentage of mature or immature spermatozoa. 200 spermatozoa were counted in each slide. The percentage of positive aniline blue (AB+) and toluidine blue (TB+) significantly decreased following varicocelectomy (59.98% versus 57.08%, P=0.047 for TB+ and 57.08%5 versus 43.91%, P=.039 for AB+). Morphology, count and motility of sperm showed improving after varicocelectomy but motility of sperm was not considered statistically significant This study suggests that nonmicrosurgical subinguinal varicocelectomy can improve human sperm DNA integrity and semen parameters in men with varicocele.