جستجوی مقالات مرتبط با کلیدواژه « Artemia » در نشریات گروه « پزشکی »

Mangrove ecosystems have been recognized to include a wide range of secondary metabolites, which are biochemically distinct, resulting in a diverse range of natural compounds with unique bioactivity. They have active metabolites with new chemical structures from a variety of chemical classes.

This study was undertaken to evaluate the phytochemical screening and cytotoxicity of four major mangrove plants (Excoecaria agallocha L., Acrostichum aureum L., Aegiceras corniculatum L., and Avicennia officinalis L.). 

This experimental study was held in the Biochemistry and Molecular Biology Laboratory of Khulna University, Bangladesh, in 2016. At the first phytochemical screening of the selected plants was observed. Then, the bioactivity as preliminary cytotoxic activity was performed using brine shrimp lethality (BSL) bioassay where a significant 50% Lethal Concentration (LC50) was exerted using polar solvent (ethanol) extract of different plant parts (leaf, bark, and stem). Then, Resazurin Cell Viability Assay was performed only for ethanolic leaf and bark extracts of E. agallocha using four standard bacteria (Escherichia coli ATCC 8739, Salmonella typhi ATCC 6539, Salmonella paratyphi ATCC 9150, and Staphylococcus aureus ATCC 25923).

The experimental findings showed significantly strong LC50 by ethanolic leaf and bark extracts of E. agallocha and other plants, like A. corniculatum, A. aureum, and A. officinalis showed moderate and negligible cytotoxicity, respectively. Then, the experimental findings showed significantly (P≤0.05) strong IC50 by ethanolic leaf and bark extracts of E. agallocha. 

The screens employed in this present study are preliminary and advanced assays are needed to verify and reveal further this bioactivity present in those plants, particularly E. agallocha.

Mangrove plants, often possessing a unique collection of metabolites, have extensively been used for the primary healthcare of coastal residents.

To determine the safety level and enrich the scientific data, the present study aimed to investigate the toxicity of Avicennia officinalis and Excoecaria agallocha. 

Ethanolic leaf and stem extracts were evaluated for cytotoxicity by brine shrimp lethality assay. The obtained extracts were administered to Swiss albino in a single dose (200, 400, 800, 1600, & 3200 mg/kg body weight) by oral-gavage for acute toxicity assay. Furthermore, systematic observation was performed by close monitoring for any toxic manifestations and mortality after dosing for the first 4 h, at 24 h and twice daily for 6 days. Evaluating the adverse effects were estimated by comparing the test groups with the controls. After sacrificing all group animals, relative organ weight was measured and histopathological analysis was conducted. 

Having Lethal Concentration (LC50) of 44.66 µg/mL, E. agallocha leaf was found with the highest toxicity against brine shrimp nauplii. The toxicological study data demonstrated no death and noticeable change in behavioral patterns in the test mice groups, compared with the control group. Moreover, no significant (P>0.05) differences were found in body weight and relative organ weights, compared to the controls. The histological structures of the liver in the treated mice displayed regular tissue configurations similar to the control group.

In this study, the mice model exhibited no harmful effects; thus, the reported results indicated that the ethanolic extracts of leaf and stem of these two mangrove plants are safe for therapeutic use. Further long-term toxicological impact of the extracts should be determined for well-founded confirmation.