جستجوی مقالات مرتبط با کلیدواژه « Calpain » در نشریات گروه « پزشکی »

In this study, we investigate the potential of Moricandia arvensis methanol leaf extract (MeOHL) on calpain activity, melanin biosynthesis and DNA mutagenicity. Cytotoxic effect and measurement of reactive oxygen species (ROS) induced by lucigenin in colorectal cells (BE) were also determined. In addition the chemical analysis of the extract was also studied and the chemical profile illustrates its content in para-hydroxybenzoic acid (p-HBA), a glycosylated kampferol (GK), a glycosylated kampferol with Rhamnose (GKR) and 19 amino acids (AAs). Our results showed that MeOHL extract enhanced a significant cytotoxic (max of IP = 89.23%) and antioxidant (max of IP=100%) activities. Furthermore, the tested extract stimulated calpain activity and significantly increased the production of intra (46 µg/mL cells) and extracellular melanin content (12.5 µg/mL). Using Ames assay, the extract exhibits a significant inhibition of mutagenicity induced by methy-methane-sulfonate (MMS) (76.32%) as well as 2-aminoanthracene (2-AA) (91%) in the Salmonella thyphimurium TA104 assay system.

Calpains are a family of Ca2 dependent proteases. There is some evidence that calpains involved in fusion process that occurs between spermatozoa and the oocyte. The current study aimed to investigate the association of smoking with semen quality and µ-calpain level.
This case-control study was conducted on 117 normospermia males between June 2013 and march 2014 in Jahad Laboratory in ahvaz, Iran. The semen samples were collected from male smokers (n = 50) and non-smokers (n = 67). We divided these participants as light, moderate, or heavy smokers based on their cigarettes per day (CPD). ELISA assays were used to measure µ-calpain concentration. All semen samples were analyzed according to World Health Organization guidelines.
The analysis of semen showed the volume, concentration, motility and morphology of semen were significantly lower among the smoker men than the non-smoker men. Also this significant difference was observed based on the number (light, moderate and heavy smokers) and duration (short term and long term smoker) of smoking. Although, showed no significant difference between µ-calpain of smoker men and non-smoker men. CPD showed negatively correlation with semen volume, concentration, motility and morphology of sperm.
Sperm quality was negatively correlated with CPD and duration of smoking. However, there is no significant correlation between smoking and µ-calpain concentration.

Polycystic Ovarian syndrome (PCOSS) is a common endocrine disorder with prevalence of 5-10% globally among women of reproductive age. PCOSS is being characterized by chronic anovulation، polycystic ovaries، hyperandrogenism، hirsutism، overweight، insulin resistance and infertility. It is well-known that PCOSS is a complex trait alike to Type-2 Diabetes where both genetic and environmental factors play a crucial role in pathogenesis of the disease. A couple of Type-2 Diabetes susceptibility genes including those for insulin secretion and action such as Calpain-10 showed considerable contribution to genetic predisposition of PCOSS. Since Calpain-10 gene seems to be a strong candidate gene to PCOSS، we aimed to investigate the role of Caplain-10 gene polymorphism UCSNP-44 (T/C) located in a transcription enhancer element of the gene to the disease susceptibility. We carried out a cross-sectional case-control study. Using simple random sampling ninety healthy women were selected. All ninety patients fulfilled the 2003 Rotterdam criteria of PCOSS. The subjects were genotyped for Caplain-10 gene polymorphism UCSNP-44 (T/C) using PCR-RFLP. Differences in genotype distributions between case and control subjects were examined via a chi-square test. Although PCOSS showed high prevalence among women in Bandar Abbas city، Caplain-10 gene polymorphism UCSNP-44 (T/C) UCSNP44 doesn׳ t influence susceptibility to PCOSS.