جستجوی مقالات مرتبط با کلیدواژه « Detoxification » در نشریات گروه « پزشکی »

Aflatoxins are poisonous substances produced by certain kinds of fungi that are found naturally all over the world. They can contaminate food crops and pose a serious health threat to humans and livestock. The current study aimed at removing aflatoxin from the reconstituted milk by adding three probiotics Saccharomyces boulardii, Lactobacillus casei and Lactobacillus acidophilus.

 The probiotics of S. boulardii , L.casei and L. acidophilus with 109 and 107 CFU concentration were exposed to aflatoxin M1 (0.5 and 0.75 ng/ml). The ELISA test was performed using 144 falcon tubes containing AFM1. Sterile water was added to each probiotic pellet and finally added to pre-prepare contaminated milk. After the specified times, the milk layer was analyzed to measure AFM1 levels. Each sample was analyzed using HPLC system. Subsequently, the percentage of AFM1, which was bound to the bacterial suspension, was calculated.

boulardii had the greatest ability in AFM1 removal from milk medium (96.88 ± 3.79) over time in the early hours with increasing concentration of AFM1 (0.75 ng/ml) and a concentration of 109 CFU/ml at 37 °C. The highest activity of L.casei in the removal of AFM1 toxin was observed at a concentration of 107 CFU/ml in 0.75 ng/ml AFM1 level and 37 °C. And the highest marginal estimation percentage of AFM1 removal from the milk medium at 4 °C in initial minutes belonged to L. acidophilus.

The results revealed the possibility of using S. boulardii in combination with selected strains of LAB (L.casei, L. acidophilus) in detoxification of AFM1-contaminated milk.

Aflatoxins are a group of fungal secondary metabolites generally produced by Aspergillus flavus and A. Parasiticus. The Aflatoxin contamination especially occurs during processing stages in dried fruits, after harvesting. Aflatoxin contamination of Iranian pistachios has caused an export reduction in the last years. Aflatoxins contribute to replacing thymidine with guanine at the codon 249 of P53 (tumor suppressor gene) in the human genome; this mutation in the DNA could cause liver cancer.

Methods to manage the reduction of aflatoxin contamination has great importance; the most effective treatments, including chemical (ozone and citric acid) and physical (UV-C and gamma radiation), are reviewed in this paper

In ozone (O3) treatments in which the sensitivity of aflatoxin B1 and G1 is more than aflatoxin B2 and G2, under UV-C radiation, the degradation of aflatoxin B2 and G2 to is higher than aflatoxin B1 and G1. Acidic and alkaline substances also are utilized to eliminate aflatoxins in some products.

Use of acids along with other detoxification methods such as ozone, UV- C radiation, and …, will be more effective to degrade aflatoxins in pistachio nuts.

Pistachios are good sources of some functional compounds that are essential for human health. In addition to consuming dried pistachios (salted/roasted) or used as ingredients in a variety of confectionery and cookery products, consuming fresh pistachios is also gaining a foothold in the market. This review presents pre- and postharvest operations to prevent microbial contamination and to preserve physicochemical properties of fresh and processed pistachios for extending their shelf life. There is a potential in pistachios to be contaminated with some undesirable microbes, especially aflatoxin-producing fungi, during pre- and postharvest operations. In this regard, strategies to the prevention of aflatoxin production and the decontamination of produced aflatoxin in pistachios have been of interest to researchers. Different practices including sorting, thermal processing, biological control, ozone treatment, gamma irradiation, ultraviolet irradiation, and cold plasma have been proposed for aflatoxin decontamination. Sorting out damaged pistachios is one of the most important postharvest strategies to reduce aflatoxin levels (up to 98%) that can be done manually or electronically. The majority of pistachios (~85%) are consumed as roasted form that combining roasting with lemon juice improves the elimination of aflatoxin (up to 93%). Drying and packaging are the most important methods to maintain quality and improve the shelf life of pistachios. Laminated and metallized films with vacuum or modified atmosphere are the proper packaging for pistachios.

The present study was conducted to assess the ability of probiotic bacteria and yeasts strains to reduce aflatoxin B1 (AFB1) in gastrointestinal simulated conditions. Aflatoxins are potent carcinogenic and immunosuppressive agents. Acute exposure to a high level of aflatoxins leads to aflatoxicosis, which cause rapid death due to liver failure. It is anticipated that consumption of probiotic microorganisms capable of binding aflatoxins can reduce the risk of AFB1 on human health to a certain extent.

For this purpose, the bacteria (1 × 1010 cfu/mL) and yeasts count (2 × 108 cells/mL) and AFB1 concentration (10 ppb) were adjusted. Then, the samples were incubated in the simulated medium, human gastric secretions and small intestine. The concentration of residual AFB1 was determined using enzyme-linked immunosorbent assay (ELISA). The results were statistically analyzed by SPSS 16 software.

The native isolated bacteria and yeasts in the simulated gastrointestinal tract condition showed a significant effect on AFB1 reduction (P<0.05). The AFB1 reduction ability of native probiotic microorganisms was strain dependent. The highest binding ability in bacteria belonged to Lactobacillus rhamnosus (31.14%) and at yeasts belonged to Saccharomyces cerevisiae (30.46%).

The use of probiotic strains is the appropriate biological method to reduce AFB1 in the human gastrointestinal tract. Probiotic bacteria could help to decrease the harmful effects of AFB1 in humans through enhancing the food safety.

In this study on the steelworkers, we aimed to investigate the impact of wet cupping therapy (WCT) on heavy metal levels in the blood.

This single-arm clinical trial was performed at Karabuk University Training and Research Hospital from January to August 2018. All patients received three WCT sessions once every month. Venous blood specimens were drawn prior to the first WCT session (venous 1) and following the third session (venous 2). Cupping blood samples were also obtained during the first session of WCT. Heavy metal levels were assessed by inductively coupled plasma mass spectrometry (ICP-MS) device. Thirteen metals were subject to examination: aluminum (Al), antimony (Sb), arsenic (As), cadmium (Cd), chrome (Cr), cupper (Cu), iron (Fe), lead (Pb), manganese (Mn), mercury (Hg), molybdenum (Mo), nickel (Ni), and zinc (Zn). Relationships between the blood samples were examined using Wilcoxon Signed Rank test by R statistical software.

Forty-four subjects completed the study. The levels of Al, Cd, Cr, Cu, Mn, Mo, Ni, Sb (P < 0.001 in all), Hg (P = 0.024) and Pb (P = 0.012) were significantly lower in venous 2 samples compared to venous 1, while no significant changes were observed in the levels of Fe, Zn, and As (P = 0.575, P = 0.090 and P = 0.195, respectively). When compared to venous 1 the levels of Mn, Zn, As, Sb, Hg (P < 0.001 in all), Al (P = 0.003), Cr (P = 0.004), Cu (P = 0.022) and Mo (P = 0.014) were found significantly higher in cupping blood while Cd, Pb, and Ni were not at significantly different levels (P = 0.160, P = 0.079 and P = 0.713, respectively). Fe was found significantly lower in cupping blood (P < 0.001).

This study showed that WCT has significantly reduced heavy metal levels in the blood. It might be useful in workers who work in jobs where heavy metal toxicity can be seen.