جستجوی مقالات مرتبط با کلیدواژه « Fetal Calf Serum » در نشریات گروه « پزشکی »

The protozoan parasites of the genus Leishmania are the causative agents of various clinical diseases. Different methods of cultivation of Leishmania parasites are available. In the present study, the efficacy of the LB broth with rabbit lyophilized anti-sheep red blood cell haemolysin was evaluated in the cultivation of promastigotes of Leishmania major. Conventional LB broth medium was prepared and autoclaved for 15 min at 121°C and then lyophilized rabbit anti-sheep cell haemolysin was added at 1-10% final concentrations. The efficacy of the medium was evaluated by assessing the growth ability and replication patterns of the promastigotes of Leishmania major. Medium with 1-10% lyophilized rabbit haemolysin supported the growth of the parasites and can be used for cultivation of Leishmania parasites with acceptable In vivo infectivity for research purpose. The ability of the parasites to survive and proliferate in the presence of lyophilized rabbit haemolysin indicates that this material is a good nutritional source. This study opens a new way to make low-cost medium that can be used in cultivation of Leishmania parasites.

Backgrounds and Aims: The protozoan parasites of the genus Leishmania can be stated as the causative agents of the various clinical diseases. Fetal Calf Serum (FCS) is the major part of the Leishmania culture media, which is the most expensive ingredient in these media. The coconut water is packed with nutrients that yield an array of health benefits.
In the present study, the efficacy of the coconut water was evaluated in the cultivation of promastigotes of Leishmania infantum.
The results indicated that, the coconut water enriched with culture medium could not support the growth of the parasites and cannot be used for cultivation of Leishmania infantum.
Leishmaniases are neglected tropical diseases that can cause human infections varying from self-healing cutaneous lesions to mucosal diffuse cutaneous and visceral forms. It is concluded that although the coconut water is an easy available and cheap replacement for FCS, it could not be used in cultivation process of Leishmania infantum promastigotes.

Leishmania is a single cell parasite causing leishmaniasis, which is a common disease between humans and animals. Due to the importance of in-vitro culture of the parasite in leishmania research, developing new methods for in-vitro cultivation of the parasite has always been a goal for leishmania researchers. The main objective of this study was to use sheep and bovine hydatid cyst fluids as alternatives for fetal calf serum (FCS) in leishmania in-vitro culture. A total of 1 million leishmania promastigotes were added to 4 flasks as follow. A flask containing DMEM medium with 10% fetal bovine serum, a flask containing DMEM and 10% sheep hydatid cyst fluid, a flask containing DMEM medium with 10% bovine hydatid cyst fluid and a flask containing DMEM medium alone. After 2, 4, 7, 9, 11, 21 and 24 days, the number of parasites were counted and compared. The result of this study showed that, DMEM medium enriched with 10% sheep hydatid cyst fluid in 168 hours and medium enriched with 10% bovine hydatid cyst fluid in 96 hours can act as a good alternative for fetal bovine serum in the culture Leishmania major. The results showed that sheep and bovine hydatid cyst fluid can be used as alternatives to FCS for dense cultivation of leishmania. The results also showed that, the growth of promastigotes in medium enriched with bovine cyst fluid is more rapid than the medium enriched with sheep cyst fluid in the beginning of cultivation.