جستجوی مقالات مرتبط با کلیدواژه « IL-1β » در نشریات گروه « پزشکی »

The growing threat of antibiotic resistance and Klebsiella pneumoniae infection in healthcare settings highlights the urgent need for innovative solutions, such as vaccines, to address these challenges. This study sought to assess the potential of using K. pneumoniae OmpA as a vaccine candidate through both in silico and in vivo analyses.

The study examined the OmpA protein sequence for subcellular localization, antigenicity, allergenicity, similarity to the human proteome, physicochemical properties, B-cell epitopes, MHC binding sites, tertiary structure predictions, molecular docking, and immune response simulations. The ompA gene was cloned into the pET-28a (+) vector, expressed, purified and confirmed using Western blotting analysis. IgG levels in the serum of the immunized mice were measured using ELISA with dilutions ranging from 1:100 to 1:6400, targeting rOmpA and K. pneumoniae ATCC 13883. The sensitivity and specificity of the ELISA method were also assessed.

The bioinformatics analysis identified rOmpA as a promising vaccine candidate. The immunized group demonstrated significant production of specific total IgG antibodies against rOmpA and K. pneumoniae ATCC1 13883, as compared to the control group (p < 0.0001). The titers of antibodies produced in response to bacterial exposure did not show any significant difference when compared to the anti-rOmpA antibodies (p > 0.05). The ELISA test sensitivity was 1:3200, and the antibodies in the serum could accurately recognize K. pneumoniae cells.

This study is a significant advancement in the development of a potential vaccine against K. pneumoniae that relies on OmpA. Nevertheless, additional experimental analyses are required.

Traumatic brain injury or TBI can underlie epilepsy. Prevention of PTE has been of great interest to scientists. Given the antiepileptic, antioxidant and anti-inflammatory activities of curcumin, we examined whether this compound can affect epileptogenesis in rats after TBI.

Curcumin was injected once a day for two weeks. TBI was induced in the temporal cortex of anesthetized rats using a CCI device. One day after TBI, PTZ, 35 mg/kg, was injected i.p. every other day until manifestation of generalized seizures. The number of PTZ injections was then recorded. Moreover, the extent of cortical and hippocampal IL-1β and GFAP expression in the epileptic rats were measured by Western blot analysis.

Curcumin 50 and 150 mg/kg prevented the development of kindling, wherase TBI accelerated the rate of kindling. Curcumin 20 mg/kg prohibited kindling facilitation by TBI, and reduced the expression of IL-1β and GFAP induced by TBI.

Curcumin can stop the acceleration of epileptogenesis after TBI in rats. Inhibiting hippocampal and cortical overexpression of IL-1β and GFAP seems to be involved in this activity.

 Nannorrhops ritchieana(N.ritchieana) is the sole species in the genus Nannorrhops in the palm family of Arecaceae. Regarding the use of N. ritchieana inflorescence (NER) in the folklore medicine of the south of Iran for relieving inflammation and pain in inflammation-based disorders, the current study assessed the anti-inflammatory features of N. Ritchieana inflorescence extract (NRE) in animal models.

 The carrageenan-induced paw edema method was used to evaluate theacute anti-inflammatory effects of the NRE extract (50,100, and 200mg/kg, IP). The standardand control groups received mefenamic acid and normal saline. Then, the rat’s paw tissues were removed and collected for studying tumor necrosis factor (TNF-α) level. In addition, the cotton pellet-induced granuloma method was utilized to assess the chronic anti-inflammatory effects of the extract (100 and 200 mg/kg IP). Standard and control groups received indomethacin and saline. On the last day of the cotton pellet test, the rat’s serum was collected for interleukin-1 beta (IL-1β) determination.

 In the carrageenan test, the extract significantly reduced the paw edema compared to the control (p<0.05). The extract’s anti-inflammatory activity (100 and 200 mg/kg) was the same as the mefenamic acid group. TNF-α levels demonstrated significant anti-inflammatory effects just at the dose of 200 mg/kg. In granuloma induced by cotton pellets, NRE was also effective based on the granuloma formation and the transudate amount. The anti-inflammatory activity of the inflorescence extract(100 and 200 mg/kg) was found to be the same as the indomethacin group, whereas IL-1β did not represent significant changes.

 According to the results, NRE had a considerable anti-inflammatory effect.

Context: 

This systematic review aimed to investigate the salivary biomarkers interleukin 6 (IL-6), interleukin 1β (IL-1β), and tumor necrosis factor (TNF-α) in burning mouth syndrome (BMS) to determine whether proinflammatory cytokines are effective in causing and sustaining pain due to nerve damage and whether some salivary interleukins are elevated in BMS patients.

Evidence Acquisition: 

A systematic review was conducted in Google Scholar, PubMed, and Scopus from 2000 to 2021. All information was categorized in tables.

 We found 264 articles in abstract screening. After removing duplicates, 61 articles were selected. Further screening reduced the number of articles to 28. After reviewing the full texts, finally, 15 articles had the inclusion criteria and met the objectives of the present study.

 Burning mouth syndrome is a chronic disorder with an unknown origin that involves systemic and neuropathic factors. We require further studies due to inconsistent studies and very few clinical trials deciding whether there is an association between IL-6, TNF-α, and IL-1β and BMS.

Rheumatoid Arthritis (RA) is a systemic chronic autoimmune disease. Several inflammatory agents play key roles in RA pathogenesis, among which tumor necrosis factor-alpha (TNF-α) and interleukin 1 beta (IL-1β) are of great importance. Silymarin is a potent anti-oxidant extracted from Silybummarianum L. seeds. To study the effect of silymarin on serum levels of TNF-α and IL-1β in patients with RA. Patients with stable RA received 140 mg of silymarin, 3 times a day, for 3 months. Serum samples were collected before and after the treatment. Both TNF-α and IL-1β serum levels were measured by ELISA. 42 patients (14.3% male, and 85.7% female, with a mean age of 47.59±12.8 years old) completed the treatment course. There was no significant difference in the overall mean concentration of either TNF-α (p=0.14) or IL-1β (p=0.27) in all 42 patients after the treatment with silymarin. The addition of silymarin to the treatment regimen of patients with stable RA has no significant effect on the serum levels of TNF-α and IL-1β, however, this study needs further evaluation with a larger sample size.

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) provokes the host immune responses and induces severe respiratory syndrome by overreaction of immune cells. IL-1β is a pro-inflammatory cytokine highly associated with the related inflammation and cytokine storm, and several IL-1β antagonists are being used to treat cytokine release syndrome (CRS). Accordingly, some studies and clinical trials are investigating the effects of IL-1β antagonists for controlling Coronavirus disease 2019 (COVID-19) associated CRS. Here, we will review any interaction and association between IL-1β and SARS-CoV-2 infection.

Innate immunity cytokines conduct significant functions in the stimulation and induction of liver complications in Hepatitis B infected patients. It has been reported that Royal Jelly (RJ) has important roles in decreasing the pro-inflammatory cytokines in both in vitro and in vivo conditions. This project aimed to investigate the impacts of 1 month RJ administration on interleukin 1 beta (IL-1β), tumor necrosis factor-alpha (TNF-α), and interleukin 6 (IL-6) serum levels in the Hepatitis B patients. In this research, 30 Hepatitis B cases (patients) were entered in order to be under the treatment of RJ for 1 month. Before and after treatment with RJ, Hepatitis B Virus (HBV) copy numbers were evaluated using Real-Time PCR and IL-1β, TNF-α, and IL-6 serum levels were evaluated using the ELISA technique. The RJ treatment significantly reduced the number of HBV-DNA copies and led to down-regulation of TNF-α and IL-6, which were not substantial. The IL-1β, TNF-α, and IL-6 serum levels were not changed after RJ treatment in both men and women. Based on the findings of the present study, it seems that RJ plays anti-viral and anti-inflammatory roles in the in vivo conditions in infected patients with HBV.

Inflammation in the implant-abutment interface is one of the main factors that can reduce implant stability. Therefore, this study investigated the effect of chlorhexidine, tetracycline, saliva, and a dry environment on the interleukin IL-1β and interleukin IL-6 levels of the gingival groove fluid at the implant-abutment interface.

Twenty-four (10 men and 14 women) patients referred to the Faculty of Dentistry for implant treatment, who met the inclusion criteria, were examined. Four different materials were used in each implant, including 2% chlorhexidine, 3% tetracycline, saliva, and a dry medium. Each test material was placed inside the implant screw during the anchorage session, and the healing screw was closed. Patients were then sampled in three implantation sessions and one month after prosthesis delivery. Interstitial fluid groove was used for sampling after cleaning the mouth (half an hour after three minutes of thorough brushing). The data were analyzed with SPSS 20 using ANOVA and relevant post hoc tests.

There was a significant difference in the mean IL-6 and IL-1β levels between the four materials (P<0.05). IL-6β levels were similar in tetracycline and chlorhexidine but significantly higher than in saliva and the dry environment (P<0.05). IL-6 and IL-1β levels in the saliva were significantly higher than in the dry environment (P<0.05).

The use of tetracycline at the junction of implant and abutment reduces the inflammatory cytokines IL-6 and IL-1β.

Familial Mediterranean Fever (FMF) is a hereditary auto-inflammatory disorder that is caused by mutations in the Mediterranean fever (MEFV) gene and is associated with an increase in pro-inflammatory cytokines, such as interleukin-1β (IL-1β) and interleukin-18 (IL-18), leading to excess inflammation. Colchicine is a common drug widely used for treatment of FMF attacks, but about 5–15% of the patients show resistance to the regular colchicine treatment. In this study, we used dimethylamino-parthenolide (DMAPT), as a small molecule inhibitor of Nuclear factor-κB (NF-κB), NLR family Pyrin domain containing 3 (NLRP3), and cysteine-aspartic acid protease 1(Caspase-1) on FMF-derived peripheral blood mononuclear cells (PBMCs). The effects of DMAPT and colchicine on metabolic activity and apoptosis of FMF-derived PBMCs were evaluated by MTT and Annexin V/PI assays, respectively. Also, the expression levels of NF-κB, NLRP3, MEFV, CASP1, and IL-1β mRNA were investigated using a TaqMan real-time PCR, and the protein levels of IL-1β, IL-18, and IL-37 were assessed via an enzyme-linked immunosorbent assay (ELISA) in LPS/ ATP-stimulated PBMCs. DMAPT decreased the expression levels of NFκB (0.38±0.096, P<0.0001), NLRP3 (0.39±0.12, P<0.001), MEFV (0.384±0.145, P<0.001), CASP1 (0.48±0.13, P=0.0023), and IL-1β (0.09±0.09, P<0.0001) and reduced the secretion levels of IL-1β (8.92±5.3 vs. 149.85±20.92, P<0.0001), IL-18 (135±32.1 vs. 192±22.18, P=0.01), and IL-37 (27.5±6.3 vs. 78.19±14.3, P<0.0001) as compared to untreated cells. Given the obtained results in comparison with previous research, the future clinical development of DMAPT could result in the expansion of new anti-inflammatory therapeutics for FMF disorder.

Adipose tissue plays an important role in increasing systemic inflammation and exercise training result in modulating inflammation by decreasing adipose tissue. The present study aimed to investigate the effect of 12 weeks circuit resistance training with 40, 60 and 80 percent of one-repetition maximum (1RM) on the levels of IL-10, IL-1β and body fat percent in obese postmenopausal women.

Forty four  postmenopausal women (average age of 56.07 ± 3.18 years old) randomly divided into four group (each group 11 subjects) including the control and circuit resistance training with 40 (RT40), 60 (RT60) and 80 (RT80) percentage of 1RM. Exercise training program was conducted for 12 weeks and three session per week. Blood samples collected before and after the exercise training period. The ELISA method was used to measure the levels of IL-10 and IL-1β and data analysis performed with Graphpad Prism software.

IL-10 levels in trained groups compared to control group indicated significant increase (P < 0.05), in which the increase in RT60 (P = 0.014) and RT80 (P < 0.001) groups were also significant compared to the RT40 group. In addition, significant decrease of IL-1β in RT40 (P = 0.003), RT60 (P < 0.001) and RT80 (P < 0.001) groups compared to control group were observed, as such, IL-1β decrease in RT80 group compared to RT40 (P < 0.001) and RT60 (P = 0.001) group was also significant. Moreover, different intensity circuit resistance training was associated with decrease in percent body fat (P < 0.05), which further decreases reported in the RT60 and RT80 groups.

Despite the anti-inflammatory effects of different intensities of circuit resistance training, the higher intensity of exercise training can be associated with greater effectiveness in reducing inflammation and strengthening the anti-inflammatory pathways.

Preeclampsia (PE) is a pregnancy complication with the signs of kidney damage. The effect of S-Allyl-cysteine (SAC) on inflammatory cytokines was evaluated to prevent PE-induced renal complications.

Wistar rats were divided into seven groups: 1) control, 2) PE, 3) EC, 4) PE+SAC50, 5) PE+SAC200, 6) EC+SAC50, and 7) EC+SAC200. In Groups 1-3, the rats received saline by gavage for 9 consecutive days, starting on the day 11 of gestation (G11). In Groups 4-7, the rats received SAC (50 or 200mg/kg) by gavage for 9 days, starting on G11. The rats in PE and EC groups were injected with Lipopolysaccharides on G14. The rats in EC groups were injected with pentylenetetrazol (PTZ) on G16 and G18. On G20, urine, blood, and kidney samples were collected for biochemical analysis.

In PE and EC groups, creatinine clearance, urine protein/creatinine ratio and proteinuria significantly increased compared to the control rats. Administration of SAC significantly reduced protein excretion and the protein/creatinine ratio in the urine specimen of all treated groups.The results showed significant increase in the renal concentration of IL-1β and TNF-α in the PE and EC rats. Administration of 200 mg/kg SAC significantly decreased IL-1β and TNF-α in all treated groups. SAC (200mg/kg) significantly decreased malondialdehyde and ameliorated histological changes in PE and EC groups; it also mitigated kidney dysfunctions in experimental PE and EC.

 The ameliorative effect of SAC may be mediated by its antioxidant and modulatory effects on cytokines, such as IL-1β and TNF-α.

Based on anti-inflammatory effects of Aloe vera, the effect of aqueous extract of this plant on brain edema and changes in some pro-inflammatory cytokines was investigated after traumatic brain injury (TBI).
In this study, adult male Wistar rats were divided into 5 groups: Sham, TBI, vehicle (Veh), and low dose (LA) and high dose (HA) Aloe vera. The vehicle and aqueous extract of Aloe vera were injected intraperitoneally 30 min after induction of diffuse TBI by Marmarou’s method. Brain edema (brain water content), and transforming growth factor beta (TGF-β), tumor necrosis factor alpha (TNFα), interleukin 6 (IL-6) and IL-1β levels in serum and brain were measured 24 hr after TBI induction.
Increased brain edema by TBI was reduced by both LA and HA (p<0.01 and p<0.05, respectively). IL-6 increased in the brain of TBI group compared to sham, and which was inhibited by both Aloe vera doses compared to Veh (p<0.001). The differences in the IL-6 serum levels among Veh, LA and HA groups were not significant. Increases in serum and brain IL-1β levels were reduced only in the HA group (p<0.001). Although only in the brain, TNF-α level increased after trauma, but both LA and HA inhibited it in a dose-dependent manner (p<0.01 and p<0.05, respectively) . The amount of TGF-β in the brain was reduced by both doses of the extract (p<0.001).
These results indicated that Aloe vera has a neuroprotective effect induced by reducing brain edema. The probable mechanism particularly for HA is decreasing levels of pro-inflammatory cytokines such as TGF-β, TNF-α, IL-6 and IL-1β.

 Invasive fungal infection received more and more attention because of its high mortality, Candida albicans and Aspergillus fumigatus is the most common pathogenic fungus for systematic fungal infection, A. lentulus was isolated and identified recently and named as a sister of A. fumigatus.

 The current study aimed to explore the concentration and time-dependent relationships of the virulence of fungi due to the change in the interleukin-1 beta (IL-1β) level.

 Candida albicans, A. fumigatus, and A. lentulus suspensions with a multiplicity of infection = 0, 1, 5, 10, and 20 units were used to treat mouse dendritic cells. The IL-β level was measured by enzyme-linked immunosorbent assay (ELISA) at 2, 6, 12, 24, and 48 hours after the treatment was administered.

 The main effects and interactions between the multiplicity of infection, intervention duration, and the dependent variable of IL-1β were significant. Besides, there were statistically significant differences. Only C. albicans and A. lentulus could induce IL-1β 48 hours after administration. Furthermore, the production of IL-1β induced by A. fumigatus was higher than that induced by A. lentulus and C. albicans.

 This study demonstrated concentration- and time-dependent relationships in IL-1β production by dendritic cells induced by three types of fungi. Candida albicans and A. lentulus exhibited a slow phase-in in vitro inflammation induction. The inflammatory response induced by A. fumigatus in vitro has the characteristics of a short action time and a strong toxic effect. Finally, A. lentulus is less virulent than A. fumigatus, and its inflammation-inducing time is relatively longer.

Immune system abnormalities such as inflammation, increased autoimmunity and the skewed expression of soluble mediators, including cytokines have been observed in autistic patients. The present study aimed to compare the effects of vitamin D supplementation and/or aquatic exercise training on the serum level of inflammatory cytokines, including IL-1β and IL-1RA, and stereotypic behaviors (S.B) of children with autism spectrum disorders (ASD). 

We recruited 40 children with ASD (aged 6-14) and divided them into the aquatic exercise (n = 10), vitamin D supplementary (n=10), aquatic +supplementation (n=10), and control (n=10) groups. Participants in the aquatic exercise group performed water-based activities for 10 weeks/2 sessions per week/60 min, while the participants of the supplementary group orally received 50,000 IU vitamin D3/ day, and the combined group received both exercise and supplementation, control group did not get any intervention. We evaluated the participants’ serum levels of IL-1β, IL-1RA and S.B scores at baseline and the end of the treatment.

Results revealed that all three interventional approaches improved behavioral symptoms and IL-1β serum level; interestingly, only the combined intervention could significantly affect IL-1RA.

We concluded that both aqua-based exercise and vitamin D supplements could lead to significant improvement in serum cytokine levels and behavioral problems in children with ASD.