جستجوی مقالات مرتبط با کلیدواژه « Intestinal protozoa » در نشریات گروه « پزشکی »

Determination of the prevalence of intestinal protozoan infection is a fundamental step to set up an effective control program to improve the health status of society and to establish efficient strategies. Intestinal pathogen and even non‑pathogen protozoa consider as major causes of disease in patients with gastrointestinal problems. The objective of this study is to determine the prevalence of intestinal protozoan infection in patients with ulcerative colitis (UC) in Isfahan, Iran.

The descriptive cross‑sectional study carried out from 2013 to 2018 in Isfahan, Iran. One thousand nine hundred and sixty‑five samples of feces from patients with UC collected and each sample examined using direct wet mounting with normal saline and iodine and sedimentation tests such as formol‑ethyl acetate concentration and trichrome‑staining methods.

From 655 patients, 185 (28.2%) infected with Giardia lamblia followed by Blastocystis hominis (27.3%), Endolimax nana (14.4%), Entamoeba coli (11.5%), Iodamoba butschlii (4.7%), Entamoeba histolytica (1.4%), and Chilomastix mesnili (0.6%).

This study revealed a high prevalence of infection with at least one or six non‑pathogenic and pathogenic intestinal protozoa in UC patients in the Isfahan region. Intestinal protozoa are a challenging public health problem wherever health care is limited in the area. The emergence of UC in the world results in the need to study etiologic factors. In order to obtain further information about the etiology of disease, we investigated the prevalence of intestinal protozoan infection in patients with UC in Isfahan, Iran.

Parasitic infections, especially opportunistic parasites, can cause serious problems for immunocompromised patients. In this regard, this study aimed to evaluate parasitic infections in cancer patients undergoing chemotherapy after referring to Kashani hospital of Shahrekord from January to May 2018.   In this cross-sectional descriptive study, 750 stool samples were collected from 250 cancer patients undergoing chemotherapy (three fecal samples from each patient) and sent to the Parasitology Laboratory of Shahrekord University of Medical Sciences. All samples were examined macroscopically and microscopically through the direct method and a specific acid-fast staining method. To confirm the microscopic results, DNA was extracted from isolated parasites and amplification was performed by the polymerase chain reaction with the specific primers on positive samples.   In this study, 106 (42.4%) and 144 (57.6%) patients were males and females, respectively. In addition, 187 (74.8%) and 63 (25.2%) of them were living in urban and rural areas, respectively. In terms of the education level, most patients had a low level of literacy. Further, 12 (4.8%) patients were infected with intestinal parasites that including, 7 (2.8%), and 5 (2%) samples were infected with Blastocystis hominis and Giardia lamblia, respectively.   The results of this study showed that B. hominis and G. lamblia are the most prevalent parasites in cancer patients undergoing chemotherapy. Although the prevalence of intestinal parasites in cancer patients was low, these findings should not be contrary to the need for healthcare regarding the prevention of parasitic infections in cancer patients.

In this study, some microsporidial and coccidian parasites were isolated from 103 domestic cats in the Meshkin Shahr area, northwestern Iran during the Jun 2014 to Jun 2015, and their genera were identified using parasitological methods with emphasis on their zoonotic importance. One hundred and three fecal samples of domestic cats were collected and preserved in formalin (10%) and conserved in phosphate buffer saline solution, finally examined by microscopy after formalin-ether concentration and specific staining. Preservation in dichromate potassium (2.5%) was performed for all coccidian positive samples and then sporulated coccidian oocysts were investigated. The detected parasites were Isospora spp. 6/103(5.8%). Microsporidian spores were identified in 46/103 (44.6%) of all samples post-stained by the aniline blue staining method. Microsporidial infections were more prevalent in domestic cats. Further studies are needed in the identification of microsporidial spores isolated from infected cats.

Majority of parasitic infections in rodents have zoonotic importance. This study aimed to determine the frequency and intensity of intestinal protozoa infections of rodents including Meriones persicus, Mus musculus and, Cricetulus migratorius.
This survey was conducted in Meshkin Shahr district in northwestern Iran from Mar. to Dec. of 2014. Intestinal samples of 204 rodents including M. persicus (n=117), M. musculus (n=63) and C. migratorius (n=24) were parasitologically examined. Formalin-ether concentration method was done for all of rodents stool samples and observed with light microscope. All of suspected cases were stained with trichorome staining Method. Cultivation in dichromate potassium 2.5% was carried out for all of coccidian positive samples. Acid fast and aniline blue staining methods were used for detecting of coccidian oocysts and intestinal microsporidial spores, respectively.
About 121(59.3%) of the caught rodents were generally infected with intestinal protozoa. Entamoeba muris 14(6.9%), Trichomonas muris 55(27.0%), Chilomastix betencourtti 17 (8.3%), Giardia muris 19(9.3%), Eimeria spp. 46(22.5%), Isospora spp. 4(2%) and Cryptosporidium spp. 1(0.5%) were found from the collected rodents. Microsporidian spores were identified in 63 (31%) out of the 204 collected rodents using aniline blue staining method.
Since some of the infections are zoonotic importance thus, control of rodents can be decreased new cases of the parasitic zoonoses in humans.

Intestinal parasites are still a serious public health problem in the world, especially in developing countries. This study aimed to assess the prevalence of intestinal protozoa infections and associated risk factors among schoolchildren in Sanandaj City, Iran.
This cross-sectional study involving 400 schoolchildren was carried out in 2015. Each student was selected using systematic random sampling method. Questionnaire and observation were used to identify possible risk factors. Fresh stool samples were observed using formal-ether concentration method.
Five species of intestinal protozoa were identified with an overall prevalence of 42.3%. No cases of helminthes infection were detected. The predominant protozoa were Blastocys hominis (21.3%) and Entamoeba coli (4.5%). Overall, 143 (35.9%) had single infections and 26 (6.4%) were infected with more than one intestinal protozoa, in which 23 (5.9%) had double intestinal protozoa infections and 3 (0.5%) had triple infections. A significant relationship was observed between intestinal protozoa infection with economic status, water resources for drinking uses, and the methods of washing vegetables (P Education programs on students and their families should be implemented for the prevention and control of protozoa infections in the study area.