جستجوی مقالات مرتبط با کلیدواژه « Real-TimePCR » در نشریات گروه « پزشکی »

Fusarium graminearum produces trichothecenes, such as deoxynivalenol and secondary metabolite butenolide, which cause profound health problems in humans. In this research, the effect of acetone extract of Hibiscus sabdariffa is evaluated on growth of F. graminearum and expression of TRI4 and FG08079 genes, which are involved in deoxynivalenol and butenolide biosynthetic pathways, respectively.

Minimal inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) were determined. The expression of TRI4 and FG08079 genes were evaluated by teal-time PCR technique.

The MIC and MFC for acetone extract of Hibiscus sabdariffa against F. graminearum were 200 mg/mL and 400 mg/mL, respectively. Expression of TRI4 and FG08079 genes were significantly decreased by the acetone extract of red tea.

The results showed that acetone extract of Hibiscus sabdariffa has inhibitory and fungicidal effects on F. graminearum and is effective in reducing the expression of TRI4 and FG08079 genes, which play important roles in deoxynivalenol and butenolide production.

Nanoparticles are a new generation of antimicrobials. Zinc oxide nanoparticles have attracted a great deal of interest in their medical applications. The aim of the present study was to investigate the effect of zinc nanoparticles on the expression of mrkA and fimA genes in drug-resistant K. pneumoniae.

A total of 30 clinical isolates of K. pneumoniae were collected from Sina hospital and all the isolates were identified by biochemical tests. Antimicrobial resistance pattern was determined by disk diffusion method. PCR method was used to investigate the presence of mrkA and fimA genes. Biofilm phenotypic test was performed and after conducting MIC test by micro dilution method, realtime PCR was used to study the effect of zinc oxide nanoparticle on the expression of fimA and mrkA genes.

The highest resistance rate was against cefotaxime and ceftazidime antibiotics (67%). Twenty seven isolates harbored fimA gene while 24 isolates harbored mrkA gene. Five isolates were identified as strong biofilm producers. MIC values for zinc oxide was 2500 µg/ml in all five isolates. Results of real-time PCR showed that the expression levels of mrkA and fimA genes in isolates treated with zinc oxide decreased 8.5 and 9 fold, respectively, compared with the control.

This study suggests that zinc oxide can be a suitable candidate for the inhibition of the two studied virulence genes in K. pneumoniae.

The use of herbal remedies, either in combination with conventional drugs or as an alternative, is accepted worldwide. Silymarin derived from Milk thistle has evidence-based therapeutic potency for wide spectrum of liver diseases. The current work aimed to study the immune-modulating activity of Silymarin in HCV-infected patients by measuring the effect of pure Silymarin solution on the production of IL-17 and IL-10.

Nine HCV-1a infected patients and three healthy controls were entered in this study. The mean age of patients and healthy controls were 45.53 (± 10.21) and 39.9 (±10.88), respectively. The PBMCs were isolated, cultured in 96-well plate and incubated with Silymarin solution (5µg/ml) for 24 hours. The cells and cell culture supernatant of three groups including patients treated with Silymarin, non-treated patients, and healthy controls were then subjected to Real Time PCR and ELISA to measure the levels of inflammatory and non-inflammatory cytokines including IL-17 and IL-10, respectively. Statistical analysis was conducted using SPSS software version 20.0.

According to Real Time PCR and ELISA results, the level of IL-17 was significantly reduced in patients treated with Silymarin while the expression of IL-10 was remarkably increased in these patients.

The results of this study approved the immunomodulatory properties of Silymarin in HCV-infected patients. Hepatoprotective, antiviral, as well as immunomodulatory properties of the Silymarin make it a potential therapeutic option in patients with chronic hepatitis C.