جستجوی مقالات مرتبط با کلیدواژه « Sciatic » در نشریات گروه « پزشکی »
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سابقه و هدف
درد سیاتیک یک نوع اختلال در سیستم عصبی محیطی است که افراد زیادی را در سراسر جهان درگیر می کند. ترانس آنتول بخش فیتواستروژن مشتق از انیسون و رازیانه است که اثرات استروییدوژنیک قوی و ضد التهابی دارد. نانوذرات سریم اکسید نیز خواص آنتی اکسیدانی دارد. در این مطالعه، اثرات برهم کنش نانوذرات سریم اکسید و ترانس آنتول بر بهبود عملکرد عصب سیاتیک آسیب دیده در مدل موش صحرایی ارزیابی شد.
مواد و روش هادر این مطالعه تجربی 20 موش صحرایی نر با وزن 300-250 گرم به 4 گروه 5 تایی تقسیم شدند. گروه کنترل و گروه مدل سیاتیک، نرمال سالین دریافت کردند. به یک گروه مدل سیاتیک، تزریق همزمان mg/kg125 ترانس آنتول و mg/kg20 نانوذرات سریم اکسید و به گروه دیگر مدل ساتیک، تزریق همزمان mg/kg250 ترانس آنتول و mg/kg20 نانوذرات سریم اکسید از طریق تزریق داخل صفاقی انجام شد. آزمون های رفتاری حسی و حرکتی از هر گروه گرفته شد و بافت عضله برای بررسی میزان آتروفی خارج گردید.
نتایجتزریق همزمان ترانس آنتول و نانوذرات سریم اکسید باعث بهبود عصب سیاتیک آسیب دیده نسبت به گروه مدل سیاتیک کنترل شد. همچنین امتیاز شاخص عملکرد سیاتیک و بازیابی حسی در موش های دریافت کننده همزمان ترانس آنتول و نانوذرات سریم اکسید نسبت به مدل سیاتیک بالاتر بود.
نتیجه گیریطبق یافته های مطالعه حاضر احتمال می رود که ترانس آنتول و نانوذرات سریم اکسید به دلیل خواص آنتی اکسیدانی و نوروپروتکتیو با هم افزایی اثرات همدیگر در بازیابی عصب سیاتیک آسیب دیده دخیل باشند.
کلید واژگان: سیاتیک, ترانس آنتول, نانوذرات سریم اکسید}Feyz, Volume:27 Issue: 3, 2023, PP 236 -243BackgroundSciatica is a peripheral nervous system disorder that affects many people throughout the world. New therapeutic approaches such as nanomedicines and plant derivatives with less side effects can be used to improve nerve tissue. Trans-anethole is a part of phytoestrogen derived from anise and fennel, and has strong steroidogenic and anti-inflammatory effects. Cerium oxide nanoparticles also have antioxidant properties. In this study, the interaction effects of cerium oxide nanoparticles and trans-anethole investigated on improving the function of the damaged sciatic nerve in a rat mode.
Materials and methodIn this study, 20 male rats weighing 250-300 g divided into five groups. The control group and the sciatica model group received saline. One group of sciatica model received simultaneous 125 mg/kg trans-anethole or 20 mg/kg cerium oxide nanoparticles, and the other group of sciatica model received simultaneous 250 mg/kg trans-anethole or 20 mg/kg cerium oxide nanoparticles (IP). Behavioral tests (Sensory and motor) taken from each group and muscle tissue removed to evaluate the atrophy rate.
ResultsThe results showed that the simultaneous injection of trans-anethole and cerium oxide nanoparticles improved the damaged sciatic nerve compared to the sciatica model group. Also, the index of sciatica performance and sensory recovery was better in rats receiving trans-anethole and cerium oxide nanoparticles at the same time compared to the sciatica model group.
ConclusionTrans-anethole and cerium oxide nanoparticles, due to their antioxidant and neuroprotective properties, may be involved in the recovery of the damaged sciatic nerve by synergizing each other's effects.
Keywords: Sciatic, Trans-anethole, Cerium oxide nanoparticles} -
Objective(s)Although damaged peripheral nerves have the ability to repair, axon regeneration proceeds slowly and often poor functional results are observed. Many methods are used to repair peripheral nerve lesions, but very few have demonstrated clinical success. Hence, the intention of the present study was to explore the regenerative outcomes of cobalt ferrite nanoparticles coated with sumac on rat sciatic nerve injury.MethodsForty male Wistar rats were separated into four groups: the sham group (surgery without damage to the nerve), the negative control (nerve compression without nanoparticle injection), the experimental group 1 (nerve compression given 10 mg/kg dose of drug), and the experimental group 2 (nerve compression given 20 mg/kg dose of drug). The sciatic nerve was then compressed one centimeter above the point where it splits into three branches, tissue and muscle sections were examined in addition to foot print and hot plate tests.ResultsWhen compared to the negative control group, the speed of recovery and restoration of sensory and motor neuron function was significantly faster in groups treated with cobalt ferrite nanoparticles coated with sumac.ConclusionsInjection of cobalt ferrite nanoparticle coated with sumac increases the speed of repair of peripheral nerve damage in rats.Keywords: cobalt ferrite nanoparticles, Sciatic, Regenerative medicine}
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Objective
To assess regenerative capacities of chitosan-nanoselenium conduit on transected sciatic nerve in diabetic rats.
MethodsA 10-mm sciatic nerve defect was bridged using a chitosan-nanoselenium conduit filled with phosphate buffered saline. In chitosan group, the chitosan conduit was filled with phosphate buffered saline solution. In sham-operated group, sciatic nerve was exposed and closed. In transected group, right sciatic nerve was transected and nerve cut ends were fixed in the adjacent muscle. The regenerated fibers were studied within 12 weeks after surgery.
ResultsThe behavioral and functional and electrophysiological tests confirmed faster recovery of the regenerated axons in chitosan-nanoselenium conduit group compared to chitosan group (p=0.001). The mean ratios of gastrocnemius muscles weight were measured. There was statistically significant difference between the muscle weight ratios of chitosan-nanoselenium conduit and chitosan groups (p=0.001). Morphometric indices of regenerated fibers showed number and diameter of the myelinated fibers were significantly higher in chitosan-nanoselenium conduit group than in chitosan group.
Conclusionchitosan-nanoselenium conduit resulted in acceleration of functional recovery and quantitative morphometric indices of sciatic nerve.
Keywords: Peripheral nerve repair, Sciatic, Chitosan-nanoselenium conduit, Local} -
ObjectiveTo assess the effect of locally administered verapamil on transected peripheral nerve regeneration and functional recovery.MethodsSixty male healthy white Wistar rats were divided into four experimental groups (n=15), randomly: In transected group (TC), left sciatic nerve was transected and stumps were fixed in the adjacent muscle. In treatment group defect was bridged using chitosan tube (CHIT/Verapamil) filled with 10 µL verapamil (100ng/mL). In chitosan conduit group (CHIT), the tube was filled with phosphate-buffered saline alone. In sham-operated group (SHAM), sciatic nerve was exposed and manipulated. The repair trend was examined based on behavioral and performance tests as well as the variations of the gastrocnemius muscle, morphometric indices, and immunohistochemical indices.ResultsSciatic nerve functional study, muscle mass and morphometric indices confirmed faster recovery of regenerated axons in CHIT/Verapamil than CHIT group (P=0.001). When loaded in a chitosan tube verapamil accelerated and improved functional recovery and morphometric indices of sciatic nerve. Immunohistochemical analysis revealed the S-100 protein was vastly present in the transverse nerve sections and the myelin sheath. In the treatment group (chit/verapamil), the immunohistochemical susceptibility of the axons being repaired and the axons in the myelin sheath to S-100 protein was higher than the other groups.ConclusionThe present study demonstrated that a single local application of verapamil could accelerate functional recovery after transection of sciatic nerveKeywords: Peripheral nerve repair, Sciatic, Verapamil, Chitosan nanofiber}
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ObjectiveTo determine the effects of chitosan-zinc oxide nanocomposite conduit on transected sciatic nerve in animal model of rat.MethodsSixty male White Wistar rats were used in this study. A 10-mm sciatic nerve defect was bridged using a chitosan-zinc oxide nanocomposite conduit (CZON) filled with phosphate buffered saline. In chitosan group (CHIT) the chitosan conduit was filled with phosphate buffered saline solution. In sham-operated group (SHAM), sciatic nerve was exposed and manipulated. In transected group (TC), left sciatic nerve was transected and nerve cut ends were fixed in the adjacent muscle. The regenerated fibers were studied within 12 weeks after surgery.ResultsThe behavioral and functional tests confirmed faster recovery of the regenerated axons in CZON group compared to Chitosan group (pConclusionChitosan-zinc oxide nanocomposite conduit resulted in acceleration of functional recovery and quantitative morphometric indices of sciatic nerve.Keywords: Peripheral nerve repair, Sciatic, Chitosan-zinc oxide nanocomposite, Local}
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ObjectiveTo determine the effects of methylprednisolone-laden hydrogel loaded into a chitosan conduit on the functional recovery of peripheral nerve using a rat sciatic nerve regeneration model was assessed.Methods10-mm sciatic nerve defect was bridged using a chitosan conduit (CHIT/CGP-Hydrogel) filled with CGP-hydrogel. In authograft group (AUTO) a segment of sciatic nerve was transected and reimplanted reversely. In methylprednisolone treated group (CHIT/MP) the conduit was filled with methylprednisolone-laden CGP-hydrogel. The regenerated fibers were studied within 16 weeks after surgery.ResultsThe behavioral, functional and electrophysiological studies confirmed faster recovery of the regenerated axons in methylprednisolone treated group compared to CHIT/Hydrogel group (pConclusionMethylprednisolone-laden hydrogel when loaded in a chitosan conduit resulted in improvement of functional recovery and quantitative morphometric indices of sciatic nerve.Keywords: Peripheral nerve repair, Sciatic, Methylprednisolone, Hydrogel, Local}
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ObjectivesTo study the functional, histopathological and immunohistochemical effect of cyclosporine A on sciatic nerve regeneration using allografts in a rat sciatic nerve model.MethodsThirty male white Wistar rats were divided into three experimental groups (n = 10), randomly: Normal control group (NC), allograft group (ALLO), CsA treated group (ALLO/ CsA). In NC group left sciatic nerve was exposed through a gluteal muscle incision and after homeostasis muscle was sutured. In the ALLO group the left sciatic nerve was exposed through a gluteal muscle incision and transected proximal to the tibio-peroneal bifurcation where a 10 mm segment was excised. The same procedure was performed in the ALLO/ CsA group and the animals were treated with interaperitoneal administration of cyclosporine A. The harvested nerves of the rats of ALLO group were served as allograft for ALLO/ CsA group and vice versa. The NC and ALLO groups received 300 μL sterile olive oil interaperitoneally once a day for one week and the ALLO/ CsA group received 300 μL CsA (1mg/kg/day) interaperitoneally once a day for one week.ResultsBehavioral, functional, biomechanical and gastrocnemius muscle mass showed earlier regeneration of axons in ALLO/ CsA than in ALLO group (p=0.001). Histomorphometic and immunohistochemical studies also showed earlier regeneration of axons in ALLO/ CsA than in ALLO group (p=0.034).ConclusionAdministration of CsA could accelerate functional recovery after nerve allografting in sciatic nerve. It may have clinical implications for the surgical management of patients after nerve transection in emergency conditions.Keywords: Peripheral nerve repair, Sciatic, Cyclosporine A, Allograft}
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ObjectiveTo assess the effect of combination of pulsed electromagnetic fields (PEMF) with predatory stress on transected sciatic nerve regeneration in rats.MethodsIn sham- operated group (SOG) the nerve was manipulated and left intact. The 10-mm rat sciatic nerve gap was created in rats. In transected group (Transected) nerve stumps were sutured to adjacent muscle and in vein graft group (VG) the gap was bridged using an inside-out vein graft. In VG/PEMF group the transected nerve was bridged using vein graft, phosphate buffered saline was administered into the graft and the whole body was exposed to PEMF. In VG/PS group the transected nerve was bridged using vein graft, phosphate buffered saline was administered into the graft and the rats underwent predatory stress (PS). In VG/PEMF/PS group the transected nerve was bridged using vein graft, phosphate buffered saline was administered into thegraft, the whole body was exposed to PEMF and the rats underwent predatory stress. The regenerated nerve fibers were studied within 12 weeks after surgery.ResultsFunctional, gastrocnemius muscle mass findings and morphometric indices confirmed faster recovery of regenerated axons in VG/PEMF and VG/PEMF/PS groups compared to those in the other groups (p=0.001). The whole body exposure to PEMF improved functional recovery. Predatory stress did not affect nerve regeneration in the animals undergone predatory stress (p=0.343).ConclusionPulsed electromagnetic fields could be considered as an effective, safe and tolerable treatment for peripheral nerve repair in clinical practice.Keywords: Nerve regeneration, Sciatic, PEMF, Predatory stress, Functional recovery}
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Effect of local administration of platelet-derived growth factor B on functional recovery of peripheral nerve regeneration: A sciatic nerve transection modelBackgroundEffects of platelet-derived growth factor B (PDGF-B) on peripheral nerve regeneration was studied using a rat sciatic nerve transection model.Materials And MethodsForty‑five male, white Wistar rats were divided into three experimental groups (n = 15), randomly: Normal control group (NC), silicon group (SIL), and PDGF‑B treated group (SIL/PDGF). In NC group, left sciatic nerve was exposed through a gluteal muscle incision and after homeostasis muscle was sutured. In the SIL group, the left sciatic nerve was exposed the same way and transected proximal to tibio‑peroneal bifurcation leaving a 10‑mm gap. Proximal and distal stumps were each inserted into a silicone conduit and filled with 10 μL phosphate buffered solution. In SIL/PDGF group, the silicon conduit was filled with 10 μL PDGF‑B (0.5 ng/mL). Each group was subdivided into three subgroups of five animals each and were studied 4, 8, 1 weeks after surgery.ResultsBehavioral testing, sciatic nerve functional study, gastrocnemius muscle mass, and histomorphometric studies showed earlier regeneration of axons in SIL/PDGF than in SIL group (PConclusionLocal administration of PDGF‑B combined with silicon grafting could accelerate functional recovery and may have clinical implications for the surgical management of patients after facial nerve transection.Keywords: Conduit, functional recovery, local, nerve repair, platelet‑derived growth factor, sciatic}
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ObjectiveTo assess the effect of heparin binding neurotrophic factor (HBNF) on sciatic nerve regeneration in animal model of rat.MethodsSeventy-five male Wistar rats were divided into five experimental groups randomly (each group containing 15 animals): Sham operation group (SHAM), autograft group (AUTO), transected control (TC), chitosan conduit (CHIT) and heparin binding neurotrophic factor treated group (CHIT/HBNF). In AUTO group a segment of sciatic nerve was transected and reimplanted reversely. In SHAM group sciatic nerve was exposed and manipulated. In transected group left sciatic nerve was transected and stumps were fixed in adjacent muscle (TC). In treatment group defect was bridged using a chitosan conduit (CHIT) filled with 10 µL (0.1 mg/mL) HBNF (CHIT/HBNF). Each group was subdivided into four subgroups of five animals each and nerve fibers were studied in a 12-week period.ResultsBehavioral, functional, biomechanical, electrophysiological and gastrocnemius muscle mass findings and morphometric indices confirmed faster recovery of regenerated axons in treatment group than in CHIT group (P=0.001). Immunohistochemical reactions to S-100 in treatment group were more positive than that in CHIT group.ConclusionLocal administration of HBNF improved functional recovery and morphometric indices of sciatic nerve. It could be considered as an effective treatment for peripheral nerve repair in practice.Keywords: Nerve regeneration, Sciatic, heparin binding neurotrophic factor}
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ObjectiveTo determine the effects of local administration of platelet rich plasma (PRP) on peripheral nerve regeneration in rat sciatic nerve transection model.MethodsForty-five male white Wistar rats were randomized into three experimental groups (n = 15): Normal control group (NC), silicon group (SIL), PRP treated group (SIL/PRP). In NC group left sciatic nerve was exposed through a gluteal muscle incision and after homeostasis muscle was sutured. In SIL group left sciatic nerve was exposed the same way and transected proximal to tibio-peroneal bifurcation leaving a 10-mm gap. Proximal and distal stumps were each inserted into a silicone conduit and filled with 10 µL phosphate buffered solution. In SIL/PRP group silicon conduit was filled with 20 µL PRP. Each group was subdivided into three subgroups of five animals each and were studied 4, 8, 12 weeks after surgery.ResultsThe animals were comparable regarding the baseline characteristics. Behavioral testing, sciatic nerve functional study and gastrocnemius muscle mass showed earlier regeneration of axons in SIL/PRP than in SIL group.ConclusionLocal administration of PRP combined with silicon grafting could accelerate functional recovery of peripheral nerve. Easily available growth factors and bioactive proteins present in PRP may have clinical implications for the surgical management of patients after nerve transection.Keywords: Peripheral nerve repair, Sciatic, Platelet rich plasma (PRP), Local administration, Functional recovery}
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BackgroundPeripheral nerve repair is often complicated by fibroblastic scar formation, nerve dysfunction, and traumatic neuroma formation. Use of silicone may improve outcomes of these repairs. In this study, we tried to evaluate effectiveness of silicone gel on rats’ sciatic nerve repair, axon regeneration and scar formation around and in the nervous tissues.MethodsThis experimental study was performed on 18 rats. They underwent bilateral sciatic nerve dissection. Then, right and left damaged sciatic nerves were sutured. In left side, silicone gel was applied. Two months later, both sides were evaluated regarding to myelin fiber diameter (µm), total fascicular area (mm2), axon diameter (µm), myelin thickness (µm), G- ratio (axon diameter/myelin thickness), connective tissue area, ratio of connective tissue area/fascicular area, neuroma and foreign body formation in liver and lungs and spleen reaction. Results of right and left sides were compared.ResultsSilicone was significantly more effective in increasing myelin thickness in the side that silicone was applied) than the control side. It was not associated with inflammation, scar formation, granuloma, and neuroma formation. No foreign body reaction occurred in liver, spleen and lungs after silicone application; but axonal regeneration did not improve with after its use.ConclusionAccording to our findings, it seems that silicone application in the cases with significant complications or in the cases that nerve graft is not possible would be an ideal option.Keywords: Silicon, Sciatic, Axon regeneration, Neuroma, Nerve Repair, Scar Formation}
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زمینه و هدف
در برخی از صدمات عصبی که امکان ترمیم اولیه وجود ندارد، روش ارجح استفاده از پیوند عصب با استفاده از عصب خود بیمار (اتوگرافت) می باشد. از نظر تئوری این احتمال وجود دارد که متعاقب پیوند معکوس عصب از خروج آکسون از شاخه های فرعی ممانعت به عمل آید. پژوهش حاضر به مقایسه دو روش پیوند عصب به صورت مستقیم و معکوس می پردازد.
روش بررسی40 عدد رت به دو گروه تقسیم شدند و مورد عمل جراحی قرار گرفتند. عصب سیاتیک ران راست آن ها به طول 5/1 سانتی متر قطع شده در گروه اول به صورت مستقیم و در گروه B به صورت معکوس بین دو سر پروگزیمال و دیستال عصب مجددا آناستوموز شدند. یک و 16 هفته پس از عمل جراحی، از رت ها اثر کف پا گرفته شد. در هفته 16 بررسی پاتولوژی و شمارش آکسون انجام شد. نتیجه این دو روش گرافت عصبی از نظر کلینیکی بر اساس ایندکس عملکرد سیاتیک (Sciatic functional index) بر مبنای اثر کف پا و از نظر پاراکلینیکی بر مبنای شمارش آکسونی مقایسه شدند.
یافته هااستفاده از گرافت عصبی به روش معمول و به روش معکوس از جنبه بالینی (SFI) در هفته اول (22/0P=) و هفته شانزدهم (87/0P=) و هم چنین از جنبه پاراکلینیکی شمارش آکسون، در هفته شانزدهم (68/0P=) تفاوت معنی داری با هم ندارند.
نتیجه گیریدر ضایعات عصبی با طول کوتاه، نتایج پیوند عصب به روش معمول و معکوس تفاوتی ندارد. نتیجه گیری در نقایص با طول بلند و نیز انسان نیاز به مطالعات بیش تری دارد.
کلید واژگان: گرافت عصب, پیوند عصب به روش معمول, اثر کف پا, ایندکس سیاتیک}BackgroundAutograft is the best option in nerve defects when end-to-end repair can not sufficiently preserve nerve continuity. Theoretically، if the severed nerve is reversely grafted، it may prevent axonal growth into nerve branches، and larger amounts of axons will reach the target organ and more satisfactory results will be obtained. In this study we aimed to compare conventional versus reverse nerve grafting.
MethodsThis study was performed in Animal laboratory of Hazrat Fatemeh Hospital from April till August 2011. We randomly divided 40 Wistar rats into two groups. We excised 1. 5 cm of the right sciatic nerve and anastomosed it conventionally between the proximal and distal ends of the nerve in rats in group A and in a reverse manner in rats in group B. The rats’ footprints were recorded in the first and 16th weeks after surgery. In week 16، the grafted nerves were removed under anesthesia for pathological examination and axon count. Subsequently، the results were compared clinically by sciatic functional index (SFI) through footprint analysis and paraclinically by axon count. A p-value smaller than 0. 05 was considered statistically significant.
ResultsConventional and reverse nerve grafting no had statistically significant differences in clinical assessment in the first and 16th weeks (P=0. 87) post-surgically and also no difference in paraclinical assessment in week 16 (P=0. 68).
ConclusionWe had no significant clinically or para clinically differences between two approaches. It should be considered that the diameter and length of nerves and muscles in human is larger than rats، so the results of nerve repair may differ in human. We suggest a study in animal model which is anatomically more similar to human.
Keywords: conventional nerve graft, foot print, functional index, nerve graft, reversed nerve graft, sciatic}
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