جستجوی مقالات مرتبط با کلیدواژه « Sperm » در نشریات گروه « پزشکی »

Age, smoking, sleep duration, sleep quality, and obesity are risk factors that can affect the amount of sperm concentration, morphology, and motility. The aim of this study is to assess the lifestyle effects: of age, smoking, sleep duration, sleep quality, and obesity on the amount of concentration, morphology, and motility of sperm.

The study utilized an analytical observational approach with a cross-sectional design. The study subjects comprised 70 male partners of infertile couples admitted to the Sekar Fertility Clinic at the Dr. Moewardi General Hospital between March and August 2022. The study assessed variables including age, body mass index (BMI), smoking status, sleep duration, sleep quality, sperm concentration, sperm morphology, and sperm motility. Furthermore, the data were analyzed using univariate, bivariate, and multivariate methods with SPSS 25 software.

The research findings demonstrate that obesity is significantly associated with abnormal sperm concentration [odds ratio (OR)=40.07, confidence interval (CI)=3.90-411.67, P=0.002]. Furthermore, moderate or heavy smoking is significantly associated with abnormal sperm concentration (OR=17.45, CI=1.83-166.15, P=0.013) and sleep quality with severe disorders (OR=5.73, CI=1.12-29.21, P=0.036). Moreover, obesity is significantly associated with abnormal sperm motility (OR=12.97, CI=2.66-63.15, P=0.002), while moderate or heavy smoking (OR=5.89, CI=1.23- 28.20, P=0.026) and poor sleep duration (OR=6.21, CI=1.43-26.92, P=0.015) also exhibit significant associations with abnormal sperm motility. However, no significant findings were observed regarding sperm morphology.

The findings of this study indicate that obesity, moderate or heavy smoking, and sleep quality have statistically significant effects on sperm concentration, while obesity, moderate or heavy smoking, and sleep duration have statistically significant effects on sperm motility. However, no statistically significant influence was observed on sperm morphology. Further research with larger sample sizes and more diverse populations is needed to validate these findings and explore other potential factors that may impact male fertility.

Vitrification is a recently introduced yet widely applied assisted reproduction technique. So far, the effects of the chemicals and devices in vitrification on sperm motility and DNA integrity are still unclear.

This study aimed to examine sperm quality, as determined by semen analysis and sperm DNA integrity when vitrified with or without cryoprotectant agents (CPAs) using pulled-glass capillaries.

Between February and June 2020, 50 infertile men from the Hue Center for Reproductive Endocrinology and Infertility, Hue University of Medicine and Pharmacy, Vietnam, were enrolled. Sperm samples, prepared using the swim-up technique, were divided into 2 groups: vitrification with CPAs (group 1) and without CPAs (group 2). Vitrified sperm samples were preserved in 10 µL pulled-glass capillaries. Motility, sperm membrane integrity, and the DNA fragmentation index were tested.

Sperm motility in vitrified media with CPAs (54.4 ± 11%) was statistically higher than in media without CPAs (51.14 ± 10.6%, p < 0.05). CPAs did not affect sperm membrane integrity or large halo ratio (71.34 ± 8.47 vs. 70.38 ± 8.11 and 50.84 ± 18.92 vs. 51.98 ± 19.44, respectively). Group 2 exhibited a lower DNA fragmentation index than group 1 after vitrification (14.2 ± 8.47 vs. 12.60 ± 9.03, p = 0.021).

Using a pulled-glass capillary for sperm vitrification, the presence of CPAs in the vitrification medium resulted in higher progressive motility and lower DNA fragmentation index than the medium without CPAs.

 The potential impact of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection on male fertility is a complex and evolving area of research. Although there is some evidence to suggest that the virus might have negative effects on semen from mild to moderate symptoms or recovery of coronavirus disease 2019 (COVID-19) and might induce epididymal-orchitis, further research is needed to definitively determine the risks.

 This study aimed to investigate the presence of SARS-CoV-2 ribonucleic acid (RNA) in semen and to determine any possible implications of SARS-CoV-2 infection on semen parameters and genital tract complications.

 Thirty-two subjects with a real-time reverse transcriptase-polymerase chain reaction (RT-PCR) cycle threshold (Ct) value of less than 20 were recruited, and semen samples were collected. Additionally, testicular discomfort and urogenital abnormality were assessed.

 No SARS-CoV-2 RNA was detected in the seminal sample of men; however, semen quality impairment was detected, especially in patients with Ct values less than 15 in the upper respiratory tract. Total sperm count, the number of motile spermatozoa, progressive motility, and the percentage of normal morphology were significantly reduced. Acute epididymo-orchitis and testicular tenderness were observed in 4 patients in the acute stage of the disease.

 This study showed no evidence of SARS-CoV-2 RNA in semen, suggesting that sexual transmission of the virus is unlikely. However, semen quality impairment was observed in patients with SARS-CoV-2 infection, particularly those with higher viral loads. Further research is needed to elucidate the mechanisms by which SARS-CoV-2 affects male fertility and develop effective interventions to protect men's reproductive health during the pandemic.

Infertility has a significant impact on the quality of lives of infertile couples, necessitating the exploration of approaches to reduce its prevalence and improve fertility chances. This review focuses on the role of tree nuts in male fertility, considering their potential effects on seminal fluid quality and sperm parameters. Nuts are rich in antioxidants such as vitamins C, D, and E, as well as minerals like zinc and selenium, which are known to have a positive influence on fertility. While numerous reviews have summarized the effects of various nutrients on male fertility, there is currently no published review specifically categorizing the effects of tree nuts on male fertility. This review aimed to fill that gap by summarizing the relevant literature on nuts and male infertility. The findings of reviewed studies suggest the beneficial effects of nut consumption on reproductive health and overall health. The improvement in lipid stability, oxidative stress, inflammation indicators, and endothelial function can potentially contribute to better reproductive health, particularly in relation to agedependent decreases in sperm quality and fertility.

The perception of Cannabis toxicity and safety in society, politics, and even scientific circles has changed dramatically in recent years. Consequently, a few questions have been raised regarding the effects of marijuana (Cannabis sativa) on the human body and psychic abilities in the short and long term. This study is undertaken in order to compare sperm cells quality before and after incubation with Cannabis (marijuana) extracts (THC- THC-COOH and CBD).

Semen samples were obtained from men (n=10) with age range between 20 to 35, regardless of their fertility status. Analyses of semen parameters (volume, sperm count, motility), were analyzed within 1 hour after collection, according to WHO criteria 2020. For accurate results, Computer Assisted Sperm Analysis (CASA) system was used to determine sperm parameters. Each sample was exposed to 1 mL; 10 µg/mL of THC, THC-COOH, and CBD dissolved in methanol. All samples were also exposed to 1 mL pure methanol as a control group to eliminate the potential effects of methanol on sperm cells. Several smears (10 µL) of each sample were taken in order to assess the functionality of sperm’s chromatin integrity by employing a chromomycin (CMA3 ) and DNA fragmentation (Acridine Orange) assays.

After treatment sperm cells with 10µL THC, THC-COOH and CBD. Total sperm’s motility was significantly reduced significantly when it was exposed to THC and CBD (P ≤ 0.001 and P ≤ 0.003 respectively). Progressive motility were decreased significantly (P ≤0.001) and the mean number of immotile sperm were significantly increased (P ≤ 0.003) after incubation with THC and CBD and respectively. However, slightly inhibition of total sperm’s motility was observed after incubation with THC-COOH.

Spermatozoa exposure to THC, CBD and THC-COOH deteriorate significantly sperm motility and should be avoided by men in reproductive age and those who undergoing ART.

The use of probiotics in the treatment of infertility is a new area of research. In this study, our objective was to examine the efficacy of probiotic supplementation on semen parameters following varicocelectomy.

We included infertile men in our study who were the candidates for subinguinal microscopic varicocelectomy. After the surgical procedure, the patients were randomly assigned into two groups: 38 individuals received probiotic  upplementation (FamiLact®), while 40 individuals received a placebo for 3 months. We compared the preoperative semen parameters with the postoperative parameters to evaluate the effects of probiotic supplementation.

A total of 78 patients were included in the study. The two groups weresimilar in terms of age, body mass index, infertility period, and semen parameters at baseline (P > 0.05). A statistically significant difference was found in sperm concentration (33.7 ± 22.5 vs. 21.1 ± 16.1 × 106/mL, P = 0.046), and the percentage of sperms with normal morphology (15.0 ± 8.9 vs. 12.0 ± 11.5, P = 0.016) at 3 months favoring the probiotic group. Although the probiotic groupexhibited higher values for semen volume and sperm motility at 3 months, the differences were not statistically significant (P = 0.897 and P = 0.177, respectively).

Our study demonstrates that the short?term use of probiotics after varicocelectomy can provide additional benefits in improving semen parameters. Probiotic supplements are cost?effective and well tolerated, making them a suitable option for enhancing the outcomes of varicocelectomy.

 The prevalence of human papillomavirus (HPV) infection in infertile men is significantly higher than that in the general population. Nevertheless, it is unclear whether the presence of HPV in semen samples is related to reduced semen quality.

 The present article aims to elucidate the possible association between HPV infection and semen parameters in Iranian infertile men.

 This cross-sectional study included semen samples from 140 infertile Iranian male partners of couples seeking fertility assessment. Standard semen analysis was performed. Sperm DNA fragmentation index (DFI), sperm chromatin maturation, anti-sperm antibodies, leukocyte count, fructose concentration, and zinc concentration were also assessed. Nested polymerase chain reaction (PCR) and INNO-LiPA were used to identify HPV DNA sequences in semen samples and to determine HPV genotype, respectively. Independent samples t-tests were used to compare sperm parameters between HPV-positive and HPV-negative groups.

 Human papillomavirus DNA was detected in 18 (12.85%) out of 140 semen samples (10% high-risk genotypes, 2.14% low-risk genotypes, and one sample was excluded). Our findings revealed that HPV18 was the most prevalent type (8.57%). According to our results, the presence of HPV DNA in semen samples was associated with higher semen anti-sperm antibodies (P = 0.001), higher white blood cell (WBC) counts (P = 0.02), and increased DFI values (P = 0.03).

 Human papillomavirus -positive patients had higher semen anti-sperm antibodies, semen WBC counts, and sperm DFI values. These results highlight the importance of careful screening for the presence of HPV in semen samples from infertile men. It should also be noted that more studies are required.

There are considerable opium addicts, particularly among workers in Iran's industrial sectors. This study aimed to investigate the effects of simultaneous exposure to excessive noise and opium addiction on adult rat sperm parameters.

In this experimental study 42 adult Wistar male rats in 6 groups were studied as follows: group 1 control, group 2 exposed to noise (100 dB) 8 h/day, group 3 received daily 50 mg/case opium solution, group 4 received daily 100 mg/case opium solution, group 5 received daily 50 mg/case opium solution and exposed to noise 8h/day, and group 6 received daily 100 mg/case opium solution and exposed to noise 8h/day. After 50 days, cauda epididymis was removed for sperm parameters examination (WHO guidelines).

In the groups receiving opium (50 and 100 mg/case), sperm count, sperm viability, and normal sperm morphology (%) significantly decreased compared to the control group. Exposure to noise and consumption of opium solution simultaneously significantly reduced the count, viability, and percentage of sperm with normal morphology in both selected doses, as well as a significant difference between groups 5 and 6 in count, viability, and normal sperm morphology(%) parameters(P<0.05).

According to the results, noise exposure and taking opium would actively reduce the count, viability, and normal sperm morphology. It is therefore suggested that the mechanism of such effects should be investigated in animal and human studies.

 Infertility affects couples worldwide, with male factors being responsible for half of all cases.

 This study aimed to investigate the relationship between dietary inflammatory index (DII) and levels of C-reactive protein (CRP) in plasma and semen with the quality of semen in infertile males.

 In this cross-sectional study, 88 infertile men referring to Besat hospital, Tehran, Iran from December 2021-November 2022 were enrolled. A detailed questionnaire requesting information, and a 168-item semiquantitative food frequency questionnaire, were completed by participants. A food frequency questionnaire was used to calculate the DII. Additionally, semen and blood samples were collected from each participant for semen analysis and CRP-level assessment. Statistical analyses were performed to explore the association between DII and CRP levels with sperm quality. The correlation between DII and serum/semen CRP, besides assessing nutrients in each DII quartile group, was also explored.

 A significant difference was observed between different DII quartiles considering sperm motility (p = 0.006) and morphology (p = 0.014). Post hoc study revealed a significant difference between the 1st and 2nd quartiles and the 1st and 4th quartiles of DII regarding sperm motility (p = 0.011, and 0.017 respectively) and a significant difference between the 1st and 2nd quartiles of DII considering sperm morphology (p = 0.009). A statistically significant inverse correlation was also observed between DII and sperm motility (p = 0.017). Carbohydrates and β-carotenes were significantly different between the 4 DII quartiles (p = 0.043 and p = 0.026, respectively). Finally, no significant correlation was observed between DII and CRP levels in blood and semen (p > 0.05).

 The findings suggested a notable correlation between DII and semen quality; however, no significant association were observed between DII and CRP levels in blood and semen.

 Saccharin is approximately 300 times sweeter than sucrose, and since its discovery, there has been several controversies regarding its potential toxicity as chronic saccharin consumption negatively influences biochemical parameters. Subsequently, Solanum aethiopicum is a good source of bioactive compounds that can be used to treat a variety of ailments including nervous, respiratory, visual, renal, circulatory and fertility issues. The aim of this study was to investigate the effects of ethanolic fruit extract of Solanum Aethiopicum (L.) on saccharin induced hyperlipidemia and sperm abnormalities in male Wistar rats.

 In this experimental study, 16 Wistar rats were divided into four groups (Groups 1 to 4) comprising of four Wistar Rats per group. Group 1 (control) was given distilled water and feed only, group 2 was administered saccharin (10 mg), group 3 were administered saccharin (10 mg) and 50 mg of extract, while group 4 was administered saccharin (10 mg) and 100 mg of extract. The extract was administered for 21 days, then the rats were sacrificed and blood sample was collected through cardiac puncture for Lipid profile test. Also, semen analysis was conducted to assess sperm count, motility, viability, and morphology. The results were expressed as mean ± SEM. The differences between the four groups were determined using one-way analysis of variance, and results were considered to be significant at p ≤ 0.05.

 The results showed that saccharin adversely affected lipid profile and sperm parameters in Wistar Rats. Also, ethanolic fruit extract of Solanum aethiopicum caused a significant dose-dependent increase of lipid profile and sperm parameters in Wistar Rats administered saccharin.

 Solanum aethiopicum is beneficial for treatment of sperm abnormalities and hyperlipidemia. It is recommended that the fruit be utilized as a less expensive alternative in clinical therapies for treating sperm abnormalities and hyperlipidemia.

Sperm cryopreservation can reduce the quality of sperm. Antioxidants can improve sperm parameters by decreasing oxidative stress.

This study was performed to determine the effect of supplementing sperm freezing media with cysteamine on the quality of sperm after freezing.

This descriptive-analytical research was conducted at Omolbanin Center for Infertility Treatment in Dezful, Iran, in 2022. The samples included 60 men with normosperm based on the World Health Organization standards. Each sample was divided into three parts: 1. fresh semen group, 2. semen frozen with 10 mmol cysteamine supplementation, and 3. semen frozen without cysteamine supplementation. Viability, motility, morphology, total antioxidant capacity (TAC), and malondialdehyde (MDA) levels were evaluated. The results were described using SPSS version 22, and the significance level was considered P  0.05.

The mean of progressive motility was significantly higher in the fresh semen group (48.3 ± 4.74) compared to the two other groups. The mean progressive motility was significantly lower in the group without cysteamine supplementation (24.4 ± 3.38) than in the group with cysteamine supplementation (35.3 ± 4.9). Moreover, TAC was significantly higher in the group with cysteamine supplementation (816.6 ± 4.36) than in the other groups.We found that theMDAlevel was significantly higher in the group without cysteamine supplementation than in the other groups (36.5 ± 2.51).

According to the results of the present study, appropriate conditions for preserving the sperm parameters after cryopreservation could be created by adding cysteamine to the sperm cryopreservation media as an antioxidant effective on sperm parameters.

Epigenetic modifications such as DNA methylation play a key role in male infertility etiology. This study aimed to explore the global DNA methylation status in testicular spermatogenic cells of varicocele-induced rats and consider their semen quality, with a focus on key epigenetic marks, namely 5-methylcytosine (5-mC) and 5-hydroxymethylcytosine (5-hmC), as well as the mRNA and proteins of ten-eleven translocation (TET) methylcytosine dioxygenases 1-3.

In this experimental study, 24 mature male Wistar rats (8 in each group) were assigned amongst the control, sham, and varicocele groups. Sperm quality was assessed, and DNA methylation patterns of testicular spermatogenic cells were investigated using reverse transcription-polymerase chain reaction (RT-PCR), western blot, and immunofluorescence techniques.

Sperm parameters, chromatin and DNA integrity were significantly lower, and sperm lipid peroxidation significantly increased in varicocele-induced rats in comparison with control rats. During spermatogenesis in rat testis, 5-mC and 5-hmC epigenetic marks, and TET1-3 mRNA and proteins were expressed. In contrast to the 5-mC fluorescent signal which was presented in all testicular cells, the 5-hmC fluorescent signal was presented exclusively in spermatogonia and a few spermatids. In varicocele-induced rats, the 5-mC signal decreased in all cells within the tubules, whereas a strong signal of 5-hmC was detected in seminiferous tubules compared to the control group. As well, the levels of TET2 mRNA and protein expression were significantly upregulated in varicocele-induced rats in comparison with the control group. Also, our results showed that the varicocele-induced animals exhibited strong fluorescent signals of TET1-3 in testicular cells, whereas weak fluorescent signals were identified in the seminiferous tubules of the control animals.

Consequently, we showed TET2 upregulation and the 5-hmC gain at testicular levels are associated with varicocele and sperm quality decline, and therefore they can be exploited as potential biomarkers of spermatogenesis.

The root of Waltheria indica is used in Nigeria to enhance libido. This study evaluated the aphrodisiac potential and toxicological profile of ethanol root extract (EEWI) in male Wistar rats.

The aphrodisiac potential was evaluated on the 7th and 14th days of oral administration of graded doses (100, 200, and 400 mg/kg/day) of EEWI. Anxiolytic properties were evaluated on the 7th day, while memory retention properties were evaluated on the 14th, 21st, and 28th days. Sperm parameters, toxicological profile, and antioxidant potential were evaluated on the 28th day. The in vitro antioxidant property was also evaluated.

In a dose-dependent manner, the extract increased mounting frequency and intromission frequency but significantly decreased mount and intromission latencies (p<0.001) on the 7th and 14th day of treatment. It prolonged ejaculatory latency.  Anxiolytic and memory studies showed that the extract compared agreed with standards. Toxicologically, it was observed that all the doses used neither caused death nor any gross toxicological symptom.  A significant increase (p<0.05) was observed in the concentrations of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPX), as well as hematological parameters such as red blood cells and hemoglobin. A significant reduction in total sperm and motile sperm cell counts was observed in rats given the highest dose.

The extract possesses aphrodisiac action and is relatively safe. The antioxidant property may accentuate the aphrodisiac and anti-anemic use in ethnomedicine.

Sperm chromatin abnormalities are defects in nuclear maturation and DNA integrity. These defectsoriginated from defective spermatogenesis due to a lack of DNA repair during chromatin remodeling. Changes insemen elements can cause damage to chromatin. There is little information about the relationship between changesin trace metal elements and total antioxidant capacity (TAC) with sperm chromatin damage. The present study wasconducted to determine the relationship between Selenium (Se), Iron (Fe), Zinc (Zn), Copper (Cu) and the TAC ofsemen with the status of human sperm chromatin.

In this experimental study, semen samples (n=30) were collected from healthy men referredto Kermanshah Motazadi Hospital and stored in liquid nitrogen; after thawing and centrifugation, sperm were separated.The atomic absorption method was used to measure the concentration of metal elements. The TAC was evaluatedusing the ferric-reducing antioxidant capacity of the plasma method. Furthermore, the integrity of sperm chromatinwas measured using the sperm DNA fragmentation (SDF) method.

The status of sperm chromatin had a non-significant correlation with body mass index (BMI, P=0.25, r=0.21)and a non-significant negative correlation with sperm count (P=0.71, r=-0.71) and motility (P=0.75, r=0.61). In addition,there was no significant relationship between sperm chromatin and the TAC of semen (P=0.92, r=0.01). Additionally,there was no significant correlation between Se, Zn, or Cu concentration (P>0.05) and Fe concentration,which had a partially positive relationship with the chromatin state of sperm (P=0.24, r=0.20).

The trace metal elements in the seminal fluid did not play a significant role in the status of sperm chromatin.

In Brazil, donor anonymity is mandatory; however, the tendency of Brazilians towards the practice is unknown. In this study, an attempt was made to investigate whether couples undergoing assisted reproductive technology (ART) have a different perception of anonymous versus identity-release gamete donation than a target population in Brazil.

This cross-sectional study was performed from September 1, 2020 to December 15, 2020. For that purpose, surveys through online platforms were conducted, including either patients undergoing ART (ART-group, n=400) or subjects interested in the theme (interested-group, n=100) randomized by age at a 1:4 ratio. The survey collected information on the participants’ attitudes towards anonymity of gamete donors, and answers were compared between the groups.

Most participants stated that the relationship between children and their parents would be affected by the child's knowledge of the origin of its conception. Most participants in the ART-group believed that the gamete donor’s identity should not be revealed to the child, while only half of the interested-group stated the same. Most of the participants stated that "the donor's identity should be revealed if the child questions its biological origin". "From birth" was the second most common response, while "when the child turns 18 years old" and "sometime during teenage years" were less common answers.

The attitudes of ART patients about anonymity are conservative, with most participants believing that family relationships may be affected if the child is aware of the origin of his/her conception. These patients also believe that the identity of the gamete donor should not be revealed to the child.