جستجوی مقالات مرتبط با کلیدواژه « Sperm quality » در نشریات گروه « پزشکی »

This study evaluated the effect of ethanol extract of Aju Mbaise (EEAM) on sperm morphology, semen quality, sex hormonal levels, gonadosomatic index and testicular histology of Avodart-induced rats.

The study had normal control, Avodart control, EEAM control, Avodart+500mg/kg EEAM, and Avodart +1000mg/kg EEAM groups. The rats were orally administered 0.5 mg/kg Avodart and 1 hour later treated with EEAM for 28 consecutive days. The Avodart control had milky white semen with low Ph, volume and semen consistency compared to the normal control and EEAM treated rats with creamy white semen with higher pH and semen consistency, respectively.

The results indicated a substantial decline in spermatozoa mass motility; spermatozoa live proportion, spermatozoa concentrations, normal spermatozoa proportions, animal live weight, paired testes weight and relative testicular weight in the Avodart control compared to the normal control. The Avodart induction caused a significant reduction in the serum testosterone, follicle-stimulating hormone and luteinising hormone levels compared to the normal control. Also, there was a substantial decline in spermatogenic activity and the absence of mature spermatocytes in the lumen of the seminiferous tubules in the Avodart control relative to the normal control and EEAM control. The alterations in the sperm morphology, semen quality, sex hormonal levels and gonadosomatic index were significantly reverted to normal in the EEAM treated Avodart induced rats in a dose-dependent manner compared to the Avodart control.

These findings showed that EEAM ameliorates Avodart toxicity and improves fertility by restoring sperm quality and sex hormonal levels to normal in rats.

To explore the effectiveness of vitamin E in male infertility, a systematic review and meta-analysis was conducted.

The retrieval time was from January 1947 to May 2021, without language restriction. Stata 12.0 was used for the meta-analysis.

A total of 8 randomized controlled trials involving 459 patients were included. The results showed that af-ter vitamin E treatment, semen volume was reduced (95% CI: - 0.55 to - 0.06, SMD = - 0.30, p = 0.015), total sperm count was increased (95% CI: 0.02-0.45, SMD = 0.23, p = 0.035), and the differences were statistically significant. There were no statistically significant differences in increasing sperm concentration (95% CI: -0.21-0.29, SMD = 0.04, p = 0.769), total sperm motility (95% CI: -0.01-0.42, SMD = 0.20, p = 0.061) or sperm forward motility rate (95% CI: -0.06-0.65, SMD = 0.29, p = 0.106). Subgroup analysis showed that vitamin E treatment for six months could improve sperm forward motility (95% CI: 0.46-1.14, SMD = 0.80, p <0.001).

Vitamin E could increase the total sperm count and reduce the volume of semen in male infertility patients, and long-term treatment could improve the forward motility rate of sperm. The decrease of semen volume may be the result of different abstinence time before and after the test

Normal exercise can improve human physical abilities, but strenuous exercise can damage human cells. Strenuous exercise causes oxidative stress to the body. In order to determine the level of oxidative stress, it is important to check the levels of Superoxide Dismutase (SOD) and Malondialdehyde (MDA) in the body. SOD is the first line of defence in fighting against the oxidative stress, whereas MDA is the result of oxidative stress cell damage in the body. The sperm cell is the one that is affected by oxidative stress. This research aimed to investigate the differences in the effects of acute and chronic strenuous exercise on SOD production, MDA, and sperm quality. The research was based on experimental design with post-test only control group design with Wistar rats. Eighteen male Wistar rats were randomly divided into 3 groups (n = 6). Group I: Normal control, Group II: Rats were treated to swim for about 25-40 minutes until they were drowning for 1 day (acute strenuous physical exercise), and Group III: Rats were treated to swim for about 25-40 minutes until they were drowning for almost every day for 2 weeks (chronic strenuous physical exercise). Examination of SOD and MDA levels was done using spectrophotometry, examination of sperm quality was done by looking at the morphology, motility, and sperm quantity through the light microscope at x1000 with haemocytometer. Chronic strenuous exercise significantly affects the decreasing SOD levels, increasing MDA levels, and decreasing sperm quality compared to the control group and acute strenuous exercise (P < 0.05). According to the results of this study, the chronic strenuous exercise effects increase oxidative stress and sperm damage.

 Coronavirus disease 2019 (COVID-19) pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) may lead to the infertility of men.

 This study aimed to investigate the impact of COVID-19 infection on sperm parameters and reproductive hormones in fertile men.

 A total of 100 males were selected and divided into two groups: (1) patients in convalescence (patients suffering from COVID-19 infection in pharyngeal swab in accordance with reverse-transcription polymerase chain reaction [RT-PCR] or antibodies); (2) negative control group (without antibodies). Semen and blood samples were gathered from all subjects. In the native semen, immunoglobulin (Ig) M and IgG antibodies in the blood were confirmed, and COVID-19 was detected via RT-PCR. To this end, based on the World Health Organization (WHO) guidelines, semen analysis, total antioxidant capacity (TAC), and sperm DNA integrity were assessed.

 Results demonstrated that sperm concentration, motility, sperm viability, and TAC significantly reduced in fertile males with virus infection. In comparison with the control group, sperm DNA integrity was significantly increased (P < 0.05). Data indicated that the semen volume was not significantly correlated with COVID-19, and there was a significantly negative correlation between sperm concentration, sperm total motility, sperm vitality, sperm normal forms, and TAC with COVID-19. Sperm DNA fragmentation index had a significant and positive correlation with COVID-19 (P < 0.05). In addition, reproductive hormones significantly reduced in fertile males with COVID-19 infection (P < 0.05).

 COVID-19 infection has a negative influence on sperm parameters and reproductive hormones in fertile males.

This study aimed to explore whether the addition of a cyclic adenosine monophosphate (cAMP) analog and isobutylmethylxanthine (IBMX) in freezing media improved sperm quality and what role cAMP has in this recovery.

ach semen sample was cryopreserved into four groups: fresh semen sample, as a control group, freezing medium + 2.5 mM cAMP analog and 0.2 mM IBMX, freezing medium + 12.5 mM cAMP analog and 0.2 mM IBMX, and freezing medium + 25 mM cAMP analog and 0.2 mM IBMX. Sperm parameters after post-thaw were analyzed according to WHO instruction (2010). Viability, acrosome reaction, and DNA damage levels of the samples were evaluated.

Our results indicated that the effective concentrations of 12.5 and 25 mM cAMP analog and 0.2 mM IBMX significantly improved the total motility, progressive motility, and viability of the frozen-thawed (P<0.05). However, non-progressive motility and immotile were significantly reduced in the 12.5 and 25 mM cAMP analogs and 0.2 mM IBMX groups after thawing (P<0.05). During freezing the spermatozoa, the high concentration of the cAMP analog increased acrosome reaction after thawing in the 25 mM and 0.2 mM IBMX treated samples (P<0.05). DNA fragmentation in 25 mM cAMP analog and 0.2 mM (IBMX) supplementation was significantly lower compared to the other groups (P<0.05).

Our findings revealed that in vitro cAMP analog and IBMX supplementation in freezing media play an important role in preventing cryodamage by maintaining the sperm functional parameters.

Organophosphate compounds (OPCs) are suspected to make changes in reproductive function by oxidant/antioxidant balance disruption in the brain, consequently impairing hypothalamic, pituitary endocrine functions, and gonadal processes. The aim of this study was to investigate the effects of occupational exposure to OPCs on the reproductive system of farm workers, in Hamadan, Iran.

A comparative study was conducted in rural farmers and urban men aged 20–40 years. After sampling and analysis of semen quality parameters (such as sperm count, sperm motility, progressive sperm motility, and sperm morphology), serum butyrylcholinesterase (BChE) activity (a specific biomarker in OPCs exposure), as well as total antioxidant capacity, nitric oxide, and lipid peroxidation levels for both semen and serum samples were determined. In addition, serum samples were analyzed for reproductive hormones, including follicle‑stimulating hormone, luteinizing hormone (LH), and testosterone.

Our findings showed that the number of sperms (P = 0.04), their motility (P < 0.001), and progressive status (P < 0.001) in rural farmers were significantly lower than the urban population. In addition, a significant decrease was observed in BChE activity (P < 0.001) and LH level (P < 0.001), and also a remarkable increase was found in testosterone level (P = 0.0014) in the serum of rural farmers compared to the urban population. Along with a decrease in semen total antioxidant capacity, a positive significant correlation was found between sperm motility and semen antioxidant capacity (r = 0.45; P < 0.05).

Exposure to OPCs may affect reproductive outcomes through impairing hypothalamic and/or pituitary endocrine dysfunctions and gonadal processes in farmers.

Male infertility has been reported following long-term sulfasalazine, however, the precise effects of co-trimoxazole on sperm quality is controversial.

In this study, we evaluated the effects of co-trimoxazole and its co-administration with folic acid on sperm quality and histological changes of testes in male rats.

In this experimental study, 136 male Wistar rats were divided into 9 groups: I (control), II (vehicle) received saline, III: received folic acid (1 mg/kg /daily i.p., and IV- IX received co-trimoxazole (30, 60, and 120 mg/kg/daily; i.p.)纉 acid (1 mg/kg/daily; i.p.) for 14 or 28 days. Sperm samples were obtained from each group at the end of 14th and 28th days. Sperm numbers, motility, and viability were evaluated on a hemocytometer. Hematoxylin and Eosin stained testes were done for evaluation ofthe number of Leydig cells, vascularity, spermatids, spermatocytes, and means of seminiferous tubules diameter under light microscopy.

Co-trimoxazole treatment for either 14 or 28 days caused a significant decrease in the percentage of sperm number, motility, and viability (p

The data showed the adverse effects of co-trimoxazole on sperm quality and testes morphology which was protected partially by folic acid co-administration in rats. The underlying mechanism (s) needs further investigations.

Olive (Olea europaea), from the Oleaseae family, is a very popular plant for its biological and pharmacological characteristics. Olive tree derivatives possess antioxidant, anti-inflammatory, antihyperlipidemic, and cardioprotective effects.
The current study aimed at evaluating the effect of olive leaves extract (OLE) on sperm quality and antioxidant capacity in testis of rats exposed to rotenone (ROT).
In the current experimental study, 36 male Wistar rats were divided into 6 groups (n = 6). Control, Sham, ROT, and 3 ROTs treated with OLE (75, 150, and 300 mg/kg/day). ROT 3 mg/kg/48 hours was injected subcutaneously (s.c), and vehicle or OLE was administrated by oral gavage for 30 days. At the end of the study, animals were scarified and their testis tissue and epididymis samples were removed and their sperm parameters (viability, motility, and sperm count), total antioxidant capacity (TAC), and malondialdehyde (MDA) level were measured.
The results showed a significant decrease in sperm parameters and TAC in the ROT group. MDA level increased in ROT group compared to that of the control group. The 30 day administration of OLE significantly improved the sperm qualitative parameters and TAC, and decreased the elevation of MDA in the treated groups (P value In conclusion, the antioxidant effects of OLE significantly increased sperm quality and TAC, and decreased MDA level in the testis of rats exposed to ROT. These results revealed that OLE improved the sperm quality and suggested that OLE may treat infertility.

Previous research has documented that sexual abstinence can improve sperm quality. Sexologists have reported that sperms stored in a latex condom decrease at a rate of 60% per minute.
The objective of the current review is to explore the relationship between sexual abstinence duration and other factors that affect standard and substandard semen production.
Design: A scientific review of published literature.
Main Outcome Measure: Specific focus is to discuss how sexual abstinence behaviours relate to the production, quality and efficiency of sperms and semen in relation to the different characteristics such as duration of abstinence, age, and psychological behaviours. The study will also seek to verify if there is a link between sexual abstinence and semen production or what triggers semen production in men.
From the reviewed literature, data analysis on oligozoospermic samples indicated a peak motility of 30% after 1 day of abstinence. After 2 days of abstinence, the recorded average percentage of the normal sperm morphologies among the moderate and mild oligozoospermic samples ranged from 7% to 8.5%. The increase of the normal sperm morphology was statistically significant (P The data obtained supports the literature on abstinence in facilitating male infertility treatment. Thus, the data argues that so as to present the best semen samples for fertility treatment, men should collect semen sample after 3-8 days of abstinence as recommended by the World Health Organization (WHO). Patients that present normal sperms give sperm donations for cryopreservation ought to be informed not to exceed the 10-day abstinence duration as the quality of the sperms is compromised.

The aim of the study was to assess the effect of four repeated ejaculations on the same day at two-hour intervals on conventional and functional semen parameters.
Three healthy men (32±3.6 years) donated the first semen samples after 3-4 days of sexual abstinence followed by three subsequent samples on the same day at two-hour interval each. Semen samples were processed and analyzed according to the World Health Organization (WHO) 2010 guidelines. Furthermore, intracellular reactive oxygen (ROS) production, sperm DNA fragmentation and mitochondrial function were evaluated by flow cytometry.
An overall decreasing trend was noted in the conventional semen parameters at second, third and fourth evaluations after two hours of abstinence in comparison to first evaluation after 3-4 days of abstinence. The statistical comparison of the conventional semen parameters at fourth evaluation after 2 hr of abstinence revealed significant reduction (p The findings of the present study indicate the potential use of additional semen samples with repeated ejaculations at short abstinence times in assisted reproduction procedures particularly from severe oligospermic men.

Sperm DNA damage is an important factor in the etiology of male infertility.

The aim of the study was to evaluate the association of oxidative stress induced sperm DNA damage with the pathogenesis of male infertility.

The study comprised a total of 66 subjects, including fertile men (n=25) and infertile men (n=41) matched by age. Seminal malondialdehyde (MDA), phospholipid hydroperoxide (PHP), superoxide dismutase (SOD), total antioxidant status (TAS) and 8-hydroxy-2''-deoxy guanosine (8-OHdG) were estimated by spectrophotometric and ELISA based methods and the association with the sperm parameters was assessed.

The percentages of motile and morphologically normal cells were significantly lower (p < 0.001, p <0.001, respectivly) in infertile men. Seminal levels of MDA, PHP and 8-OHdG were significantly higher (p < 0.001, p < 0.001, and p=0. 02, respectively) while the SOD and TAS were significantly lower (p=0. 0003, p< 0.001, respectively) in infertile men. Sperm parameters were negatively correlated with MDA, PHP and 8-OHdG while positively correlated with SOD and TAS. A positive correlation of 8-OHdG with MDA and PHP and a negative correlation with TAS and SOD were also found.

These results suggested that oxidative stress induced sperm DNA damage might have a critical effect on the etiology of infertility. Therefore, evaluation of oxidative status, antioxidant defense systems and DNA damage, together with sperm parameters might be a useful tool for diagnosis and treatment of male infertility.