جستجوی مقالات مرتبط با کلیدواژه « Trichophyton rubrum » در نشریات گروه « پزشکی »

Dermatophytosis is a significant skin disease in humans and animals, with increasing resistance to conventional treatments. This study aimed to determine the antifungal activity of petroleum ether, dichloromethane, ethyl acetate, ethanol, and hydroethanol extracts from the aerial parts of Artemisia khorassanica, Artemisia scoparia, and Artemisia vulgaris against common dermatophytic fungi, including Trichophyton rubrum, Trichophyton verrucosum, Epidermophyton floccosum, and Microsporum canis.

This descriptive study examined the antifungal activity of various extracts from the aerial parts of Artemisia khorassanica, Artemisia scoparia, and Artemisia vulgaris against fungal isolates of Trichophyton rubrum, Trichophyton verrucosum, Microsporum canis, and Epidermophyton floccosum. The aerial parts of the three Artemisia species were extracted using five solvents: petroleum ether, dichloromethane, ethyl acetate, ethanol, and 50% hydroethanol. The extracts were screened for antidermatophytic effects against the fungi mentioned above, and the minimum inhibitory concentration (MIC) was determined using the agar dilution method. The most active extracts underwent preliminary phytochemical testing.

In the initial screening, Microsporum canis and Trichophyton verrucosum showed the highest sensitivity (86.66%) and resistance (100%) to the tested extracts, respectively. Extracts from Artemisia scoparia exhibited the broadest range of activity. The most effective extracts, with an MIC of 78.12 µg/ml, were also from this plant. Hydroethanol extracts from all plant species demonstrated the least antidermatophytic activity. Preliminary phytochemical studies revealed the presence of terpenoids in all petroleum ether and dichloromethane extracts of the plant species.

Some lipophilic compounds, particularly in the petroleum ether and dichloromethane extracts of Artemisia scoparia, exhibit significant in vitro antidermatophytic activity.

Dermatophytosis is a fungal complication of keratinized skin, hair, and nail tissues, which is caused by the establishment of dermatophytosis in the tissues. Attempts for tapping and discriminating the subtypes, as well as studying the possible association of specific species with clinical forms of trichophyton isolates using RAPD-PCR with random primers is a useful tool for typing and differentiating the fungal species. This study aimed to investigate the genomic polymorphism of Trichophyton rubrum isolated from keratinized sources using the RAPD-PCR method.

In this study, 60 dermatophytosis specimens were isolated from dermatophilic patients aged 12 to 45 years referred to Khatam-al-Anbia Hospital in Tehran province and Qods Hospital in Markazi province, Iran. The samples were inoculated in Sabourodextrose agar or microbial agar medium. A total of 30 positive samples were obtained, and specific kits were used to extract trichophyton rubrum DNA. The PCR was performed using specific primers, and the RAPD-PCR method was employed for genomic analysis. Investigation of genomic polymorphism is a valuable genetic marker in the evaluation of genome structure of Trichophyton rubrum.

A total of 30 isolates of Trichophyton rubrum were isolated after screening the clinical specimens that were identified based on morphological, microscopic, and biochemical tests. These strains were divided into nine genomic groups, some of which were found in several groups, indicating the genotypic similarity of the fungi of the same species at different locations. Among the identified clusters, cluster nine obtained the highest frequency, which included 12 isolates with common kinship characteristics.

This study investigated the genomic polymorphism of Trichophyton rubrum isolated from dermatophytes specimens of patients with dermatophytosis in Tehran, Iran, using the RAPD-PCR technique with a primer. According to the results, distance and age group had a significant effect. Furthermore, genetic diversity can play a special role in the transmission and pathogenicity of this microorganism.

The ABC family of transporters, in addition to their role in cell division and storage of cell volume in fungi, they are reported to play a role in natural metabolism and drug resistance. The purpose of this study is to investigate the molecular separation and cloning of the ATP-binding Cassette (ABC) from Trichophyton rubrum.

Sixty samples of dermatophytosis were isolated from the patients. Isolates were identified by chemical and molecular tests. Afterward, PCR was performed using specific primers for the ABC gene. PCR product was cloned in the PTG19 vector. Real-Time PCR was performed to evaluate the expression level of ABC gene.

A total of 12 isolates of Trichophyton Rubrum were identified. Six isolates were positive for ABC gene. The expression of the ABC gene cloned in E. coli XL1blue was confirmed by Real time PCR.

Expression of efflux pumps considered a way of resistance to antibiotics in microorganisms. In this regard, the researchers have developed the efflux pump inhibitor compounds and used them in combination with antibiotics to combat infections. However, because of the toxicity of most of these compounds in humans, their extensive use is prohibited. Therefore, finding new inhibitory compounds with little or no toxicity is challenging.

Trichophyton rubrum is one of the major causes of dermatophytosis both in the world and in Iran. Trichophyton rubrum invades keratinized tissues (hair, skin and nails) of humans and animals. Dermatophytosis is recurrent and drug-resistant. Furthe more, it often needs expensive treatmens. In a search about new treatment modalities alongside conventional drug therapies in order to achieve a better control for dermatophytosis, we studied the effect of Low Level Laser Therapy(LLLT) using diode laser (685 and 830 nm) on Trichophyton rubrum in an in-vitro setting.

In this in-vitro study,at first we identified T.rubrum using standard methods. Then equal amounts of T.rubrum were cultured on Sabouraud Dextrose Agar with Chloramphenicol and Cyclohexamide (Scc) plates. Afterwards,the plates were irradiated in 9 group using Diode laser(BTL 2000) 685 nm (25 mW; 3,5,10,20 J/Cm2)and 830 nm (200mW;3,5,10,30,50 J/Cm2). In each group 12 plates were irradiated. Also 12 plates served as control group. Then plates were examined for inhibition of growth, macroscopic and microscopic morphology, minimum inhibitory concentrations (MIC) and genomic changes following exposure.

The results of this study indicated that the fungal growth were observed in all groups, but LLLT is effective on quality of growth of Trichophyton rubrum in a dose dependent manner. All these changes were obvious in laser groups irradiated by 685 nm and 830 nm at a fluencies ≥ 10 J/cm2. The most changes were observed at the fluence 20 J/CM2 (685nm) and 30 J/CM2 (830 nm). (p<0/001).

In this study, LLLT didn’t inhibit the fungal growth, but changes in the quality of growth of Trichophyton rubrum imply that LLLT h effects on the T.rubrum independently, and these changes probably occur on the protein synthesis level and gene expressions.