جستجوی مقالات مرتبط با کلیدواژه « nasal carrier » در نشریات گروه « پزشکی »

Staphylococcus aureus can colonize a variety of body sites such as skin, coetaneous membrane and especially the anterior nares.  The coagulase gene (coa) typing is a technique in which the organism is typed based on the polymorphic region of the coa gene. The aim of the present study was to determine the coa based typing of S. aureus isolates from health care workers in two teaching hospitals in Yasuj city.

 In the present cross- sectional study, a total of 125 S. aureus isolates were collected from the nasal nares of healthcare workers in Shahid Beheshti and Imam Sajjad Hospitals in Yasuj city. For typing of S. aureus isolates, amplification of the 3'-end region of the coa gene was performed. Finally, PCR products were digested with HaeIII restriction enzymes and then were electrophoresed on 1.2% agarose gel, and visualized under UV illumination. Bacterial strains were typed according to the DNA bands with diverse size and numbers in each strain. Data were analyzed by descriptive statistics tests.

 Amplification of the coa gene indicated single band in nine different patterns ranging approximately from 400 to 860 base pair. The coa gene with 610 bp size was seen in 36 isolates and was considered a predominant type. After digestion of PCR products with HaeIII restriction enzymes, totally 15 distinct coa gene RFLP patterns, numbered C1 to C15, were coa observed. The C3 was the most frequent coa type with 24 isolates. There is no specific types belong to wards or professions in each hospitals.

S. auras isolated from anterior nares of HCWs showed genetic diversity in their coa gene, but only a few coa gene variants were predominant. Hence no prominent coa type was detected according to the wards or types of profession, the sources of bacteria for colonization in anterior nares of HCWs may be related to the hospital, probably the outside environment.

Based on the important role of nasal carriers of Staphylococcus aureus in hospital settings and their importance in the transmission of bacterium to patients and other personnel, the aim of the present study was to determine the frequency of nasal carriers, antibiotic resistance patterns and genotyping of S. aureus isolated from personnel of Shahid Beheshti hospital in Yasuj city (Iran) using the agr typing method.
In this cross-sectional study, a total of 347 nasal samples were collected from the healthcare workers of Shahid Beheshti Hospital in order to identify S. aureus. Cefoxitin containing disks and detection of the mecA gene were used for the identification of methicillin resistant isolates. The antimicrobial susceptibility patterns of isolates were tested against 15 different antibiotics using the Disc Agar Diffusion method. The Multiplex-PCR method was used for the agr typing of S. aureus isolates.
Ninety-three (26.8%) of personnel were nasal carriers of S. aureus, among them 9 isolates were identified as MRSA. Among agr groups, agrI (40.9%) and agrIII (40.9%) were the prominent genotypes. All of the isolates (100%) were resistant to Penicillin. The resistance rate to erythromycin and co-trimoxazole were 12.9% and 5.38% respectively. Most of the isolates were sensitive to other antibiotics.
Based on the low resistance rate of S. aureus, it appears that the eradication of nasal carrier states could be possible. S. aureus agr types I and III have an important role in nasal colonization in comparison to other agr types.

Increasing frequency of Staphylococcus aureus infections and changes in antimicrobial resistance pattern have led to renewed interest in the use of lincosamide– streptogramin B (MLSB) antibiotics for treatment of infections. Since no study has focused on the molecular epidemiology of community -acquired staphylococcus aureus isolates in Iran، the aim of this study was to determine the molecular typing and prevalence of the macrolides-lincosamides-streptogramins B (MLSB) resistance in community associated s. aureus isolated from healthy students at Arak university of Medical sciences. 568 healthy students from Arak university of Medical sciences were subjected to this study. All samples were subjected to S. aureus–specific isolation procedures. D test was performed to determine various phenotypes as well as spa typing done for molecular typing of these strains. Of 568 the 84 community acquired Staphylococcus aureus، six (7%) were Methiclicin resistant Staphylococcus aureus (CA-MRSA) and 78 (93%) were Methicillin sensitive Staphylococcus aureus (CA-MSSA) of the 84 s. aureus strains، eight (9. 5%) showed constitutive resistance with spa type t660، t701، t304، t5598، t012، t3204، t084 and t1944. Two strains (2. 5%) demonstrated inducible resistance with spa type t9024، t077، two strains (2. 5%) were D test negative with spa type t084 and t1149. 72 (85. 5%) strains. Illustrated susceptible Phenotype. Among CA-MRSA isolates، two strains had constitutive resistance and four remaining CA-MRSA had susceptible phenotype The result of this study indicates that in community associated s. aureus strains، constitutive MLSB resistance rate is higher than the rate of inducible resistance. Presence of inducible resistance to clindamycin in CA-MRSA strains، warrants that D test should be performed to detect this type of resistance. All isolates with inducible and constitutive resistance and D zone negative strains had different molecular typings.

Background and Methicillin resistant Staphylococcus aureus (MRSÂ) is one of the most important nosocomial pathogen with increasing prevalence in recent three decades worldwide. The most important source of infection is spread through health care worker. This cross sectional study was performed among personell of Razi hospital in a one month period (autum 2003) to determine the perevalence of S.aureus nasal carrier and its antibiotic resistance pattern. Ânterior nasal sampling from healthy personel was done using sterile moistened cotton swab, and the sample inserted into Neutral medium (broth) and transferred to the laberatory for assessing the existence of S.aurens and antibiogram related to seven antibiotics considering the NÇÇLS criteria. This study revealed that 36% of participants had nasal clonization of S.aureus. Çarriage rate of S.aureus and MRSÂ differed in various professional groups, (about 40% in medical staff and 48% in paramedical staff). The highest rate of resistance was against Ôxacillin 83% (the indicator of MRSÂ) and the lowest was against chloramphenicole (2.8%). Resistance against Vancomycin (VRSÂ) was seen in 2 cases (5.5%) Çonclusion: Ïn this study, carriage prevalence was less than expected but similar to the prevalence in the community (20.40%). Ântibiotic resistance was unexpectedly higher than the other studies though. High prevalence of MRSÂ and specially, existence of VRSÂ & VÏSÂ must be considered very seriously. Ït can be the result of continious exposure of organisms with various types of antibiotics and unrestricted use of antibiotics in hospitals.