جستجوی مقالات مرتبط با کلیدواژه « آویشن باغی » در نشریات گروه « دامپزشکی »

Salmonella typhimurium is one of the most important and pathogenic salmonellas that lead to infection in humans, livestock and poultry, and it causes a reduction in production and subsequent economic losses in the poultry industry. Using antibiotics to reduce side effects and kill bacteria in different doses leads to the development of resistance. In plants, the antimicrobial property is inherent in the constituent elements, so they do not accumulate in the body and do not cause the side effects of antibiotics, such as antibiotic resistance, allergic reactions, stomach problems, etc. The present study was conducted to investigate the antimicrobial effect of the herbal medicine Tymasin Plus® and the synergistic effect of this medicine with the antibiotic erythromycin on Salmonella Typhimurium infection in one-day-old chickens Ras 308. For this purpose, 40 one-day-old chickens of Ras 308 breed were used in four groups of ten. An amount of 2 ml of suspension containing 106 CFU of Salmonella typhimurium bacteria was experimentally induced to all chickens by gastric tube by layer gavage method. The experimental treatments included negative control (induction of infection but without therapeutic interventions), treatment with erythromycin antibiotic, treatment with Tymasin Plus® syrup (containing Thymus Vulgaris and Althea Officinalis extract), and treatment with the combination of erythromycin antibiotic and Tymasin Plus® syrup. The treatment started 24 hours after the induction of infection and continued until one day before sampling. On the fourth and twelfth days, after death, the liver and spleen tissues were separated for microbial nvestigations, including colony counting, Real-time PCR test, and tissue damage. The results of real-time PCR test and colony count confirmed that Tymasin Plus® syrup has an antimicrobial effect on Salmonella Typhimurium bacteria. In this study, the highest number of bacteria was specific to the control group and the lowest amount was related to the group treated with erythromycin and Tymasin Plus® ; Also, in the study of histopathology results of liver and spleen tissue, a significant difference was observed between the control group and other groups. According to this research, it was found that the herbal medicine Tymasin Plus® has the same function as erythromycin and is effective in reducing the population of Salmonella Typhimurium bacteria synergistically with erythromycin. Considering the phenomenon of antibiotic resistance, it is better to use herbal medicines or a combination of herbal medicine and antibiotic instead of using chemicals.

Thyme is one of the medicinal plants with various therapeutic properties mentioned in traditional medicine. In this research, male Wistar rats were divided in 4 experimental groups. 3 groups received Thymus vulgaris aqueous extract (1000, 2000 and 4000 microgram per microliter) intracerebroventriculary. Also in control group, normal saline (1microliter) was injected intracerebroventriculary. Then, in order to induce convulsions in all experimental groups, pentylentetrazol (80mg/kg) was injected intraperitoneally. The results showed that the injection of 1000 microgram extract significantly reduces the depth of seizure compared to the control group and causes significant increase of initiation time of fifth convulsive stage. Also, injection of 2000 microgram extract caused a significant increase of initiation time of third and fourth convulsive stages compared to the control group. On the other hand, the injection of 4000 microgram extract did not show any significant difference compared to the control group. These results show that the aqueous extract of Thymus vulgaris has anticonvulsant properties.

In order to apply the use of plant essential oils in complementary medicine, it is necessary to investigate their effects in inhibiting the growth of pathogenic bacteria in the laboratory, animal model and also consider their possible side effects. Therefore, the purpose of the present study was to investigate the antibacterial effects of garden thyme essential oil on Salmonella typhimurium and to evaluate its ability to prevent possible side effects caused by experimental exposure to the mentioned bacterium. For this purpose, 35 fourteen-day-old male broiler chickens were divided into 5 groups of 7 including healthy control, gavage control, experimental patient and experimental patient treated with garden thyme essential oil or Oxytetracycline. On the first day of the experimental period, 0.5 ml of Salmonella typhimurium microbial suspension and on subsequent days, 1 ml of garden thyme essential oil or Oxytetracycline was gavaged to all the chickens of the third to fifth groups and also 1 ml of sterile distilled water was gavage to the chickens of the second group twice a day with a time interval of 12 hours for 7 days. At the end of each stage, fecal microbial count was performed and at the end of the experimental period, all birds were euthanized for pathological examination of the small intestine and liver samples. The findings indicated that fecal bacterial shedding was observed throughout the experimental period in group 3 while in groups 4 and 5, fecal bacterial shedding was observed for 4 and 2 days after gavage respectively. The results demonstrated that Oxytetracycline treated chickens had a significant decrease in the excretion and colonization of Salmonella typhimurium.

Salmonella typhimurium is one of the most pathogenic salmonellas that lead to infection in humans, livestock and poultry. Excessive use of antibiotics in order to reduce complications and destroy bacteria in different doses leads to the development of resistance. Therefore, the use of antimicrobial compounds without side effects is considered important.

The present study was conducted to investigate the antimicrobial effect of the combination of two extracts of Thymusvulgaris and Altheaofficinalis and the synergism of this substance with erythromycin on Salmonella typhimurium infection.Materials: For this purpose, 40-day-old chicks of Ras 308 breed were used in four groups. Experimental treatments included negative control, treatment with antibiotics, treatment with TymasinPlus syrup and treatment with antibiotic and TymasinPlus. After induction of infection, on days four and twelve, after death by pity, liver and spleen tissues were isolated and tested.

The results of colony counting proved that TymasinPlus syrup has an antimicrobial effect on Salmonella typhimurium bacteria. The highest number of bacteria was specific to the control group and the lowest amount was related to the group treated with antibiotics and TymasinPlus; It was also found that TymasinPlus commercial syrup has the same function as antibiotics and is effective in reducing the bacterial population synergistically with antibiotics.

It is better to use the herbal medicine TymasinPlus instead of using chemicals when the infection caused by Salmonella typhimurium occurs.

Salmonella typhimurium is one of the main causes of food poisoning in humans of animal origin. Long-term and indiscriminate use of antibiotics has led to the development of drug-resistant pathogen strains. Therefore, it seems necessary to replace less harmful substances including medicinal plants.
The present study was conducted with the aim of investigating the antimicrobial and synergistic effect of the erythromycin and Culic®, which includes active ingredients of thyme, licorice, and anise, in order to reduce infection of S. typhimurium in broilers.
40 one-day-old Ross 308 chickens were infected with the standard strain of S. typhimurium and were divided into three treatment groups and a negative control group, and on the fourth and twelfth days after euthanasia, they were examined by colony counting method.
The highest bacterial load was observed in the control group. After that, the highest bacterial load was assigned to the culic group and erythromycin group. Finally, the lowest bacterial load was assigned to the treatment group with culic and erythromycin, which was significantly different from other groups.
Culic is effective in reducing S. typhimurium infection, and culic and erythromycin are the best methods of dealing with S. typhimurium in this study.

Thymus vulgaris is a plant of family Lamiaceae that has high antimicrobial properties. The present study was performed to evaluate the antimicrobial effects of the ethanolic extract of Thymus vulgaris on food-borne bacteria. Aerial part of Thymus vulgaris was used to prepare methanolic extract. The diameter of the growth inhibition zone of bacteria was assessed using disk diffusion. Minimum inhibitory concentration (MIC) and Minimum Bacterial Concentration (MBC) of ethanolic extract of Thymus vulgaris were evaluated on the target bacteria using ELISA plate. The diameter of the growth inhibition zone of bacteria against the ethanolic extract of Thymus vulgaris ranged from 7.87±0.61 to 16.99±0.37 mm. Application of 50 mg/ml concentration of ethanolic extract of Thymus vulgaris caused the highest diameter of the growth inhibition zone of Pseudomonas aeruginosa (11.38±0.43 mm), Escherichia coli (12.00±0.35 mm), Staphylococcus aureus (16.33±0.69 mm) and Listeria monocytogenes (16.99±0.37 mm). The antimicrobial effects of ethanolic extract of Thymus vulgaris were dose-dependent (P <0.05). The diameter of growth inhibition zone of 50 mg/ml of ethanolic extract of Thymus vulgaris on Pseudomonas aeruginosa, Staphylococcus aureus and Listeria monocytogenes was higher than all antibiotics (P <0.05). The lowest MIC and MBC values were obtained for Staphylococcus aureus (4 and 6 mg/ml, respectively) and Listeria monocytogenes (4 and 6 mg/ml, respectively). Thymus vulgaris ethanolic extract can be used as a combination with antimicrobial properties in food.

Thyme extract has the antimicrobial and antioxidant components that can be useful for the broilers under heat stress condition.

This experiment was conducted to investigate the effects of Thymus vulgaris extract in comparison with some common feed additives on performance, blood biochemical parameters and antibody response in broiler chickens under heat stress conditions.

This study was conducted using 192 one-day-old male broiler chicks (Ross 308) in a completely randomized design with 8 treatments in 4 replicates and 6 chicks per replicate. Treatments were as follows: 1) basal diet + standard temperature conditions (positive control); 2) basal diet + heat stress conditions (negative control); 3) negative control + 200 mg virginiamycin; 4) negative control + 150 mg Protoxin probiotic, 5) negative control + 250 mg vitamin C, 6) negative control + 250 mg vitamin E, 7) negative control + 250 mg thyme extract, and 8) negative control +500 mg thyme extract per kg diet.

Dietary thyme extract at the rate of 500 mg/kg as well as probiotic and vitamin E increased (P<0.05) body weight gain (2202, 2183 and 2199, respectively) and improved feed conversion (1.65, 1.74 and 1.66, respectively) compared with negative control group (body weight gain 1960 g and feed conversion ratio 1.96). Moreover, 500 mg/kg dietary thyme extract, probiotic and vitamin C reduced triglycerides, total cholesterol and low density lipoprotein during heat stress conditions (P<0.05). All dietary supplements groups significantly increased antibody titers against red blood cells as compared with negative control.

In general, the results of the present study suggest that thyme extract at the level of 500 mg/kg of diet can be recommended as an alternative to the common feed additives to improve growth performance and health benefits of the heat-stressed broilers.