جستجوی مقالات مرتبط با کلیدواژه « اسپرم اپیدیدیمی » در نشریات گروه « دامپزشکی »

Spermatozoa recovered from the cauda epididymis of dead or slaughtered animals have many potential applications in the conservation of endangered species and breeds and also in the preserving the genetic potential of valuable males that un-intentionaly culed from herds. In these cases, the animal’s testes are usually isolated from the carcase and sent to the centers capable of recovering and freezing epididymal spermatozoa. Considering the effect of post-mortem time length on the quality of recovered spermatozoa in different sheep breds and other species, this study was aimed to evaluate the influence of storing isolated epididymes of Lori-Bakhtiary rams for 24 and 48 h post-slaughter at 5 ˚C on the motility and freezablity of spermatozoa recovered at these timepoints in comparison to these parameters at 2 h post-slaughter. Before freezing, the rates of total and progresive motility at the time of 2 h were significantly higher than those of 48 h (P<0.05). Other kinemetic parameters, except for STR, were significantly different between groups (P<0.05). After thawing, the progressive motility and velocity parameters of 2 h group were significantly higher than 48 h group (P<0.05). In conclusion, the quality and freezability of ovine epididymal spermatozoa recovered from epididymes stored in refrigarator for 48 h had considerably decreased.

Cryopreservation of epididymal spermatozoa has an important role in the conservation programs of endangered domestic and wild animal species, as well as, maintaining the genetic potential of suddenly dead valuable males. The aim of the present study was setting up an optimum protocol for the cryopreservation of ram epididymal spermatozoa. Spermatozoa extracted from the cauda epididymis of slaugtherd rams were frozen in the extenders containing 2, 4 and 6 percent glycerol and were thawed at 37 and 65˚C. Post-thawing motility parameters were evaluated by CASA. The factorial ANOVA model were used to determine the statistical differences between the main effects of the glycerol concentrations, thawing temperatures and their interactions (concentration×temperature). Because a non-significant interaction was detected, the main effects (concentration and temperature) were interpreted. The effect of glycerol concentration on the all motility parameters and the effect of thawing temperature on the majority of motility parameters were significant (P<0.05). Among the glycerol concentrations, 2% had the lowest motility and 4% had the highest motility (P<0.05). Among the the thawing temperatures, thawing at 65˚C led to significantly better post-thawing motility than thawing at 37˚C (P<0.05). Finally, freezing ram epididymal spermatozoa in an extender containing 4 percent glycerol and thawing the frozen spermatozoa at 65˚C had the best outcome.