جستجوی مقالات مرتبط با کلیدواژه "تحرک اسپرم" در نشریات گروه "دامپزشکی"

Freezing sperm is a complex process that can result in various forms of cell damage. Such damage may reduce the motility and fertility of thawed sperm used for artificial insemination. In this study, fifteen semen samples were collected from five mixed-breed male dogs, and each sample was divided into three parts (control, 0.2 mmol vitamin E, and 0.4 mmol vitamin E). Although motility was observed in groups that received antioxidants, this increase was not statistically significant. However, the average motility of high-speed progressive sperm after thawing indicates a significant increase (P≤0.05) in their motility. The study showed a significant increase (P≤0.05) in high-speed progressive sperm motility, VCL, VSL, VAP, and BCF values in groups 0.2 and 0.4 compared to the control group. However, this increase was only observed in the cold shock-resistant group. In the test, the evaluation of the integrity of the sperm membrane in Group 0.4 showed a significant increase (P≤0.05) compared to the control group. The study finds that using vitamin E with concentrations of 0.2 and 0.4 improves the resistance of sperm to shocks compared to sperm that are sensitive to this phenomenon. This positive effect of vitamin E can be attributed to higher levels of unsaturated fatty acids that are susceptible to peroxidation in this group of sperm.

One of the advantages of using epididymal sperm is helping to preserve the genetic resources of elite animals and endangered species after death. This study aimed to investigate the probable protective effect of polyvinyl alcohol (PVA) and polyethylene glycol (PEG) on epididymal ram sperm under liquid storage at 4 ° C for 24 hours. Sperms were collected by making an incision on the epididymis of slaughtered rams and extended in extenders supplemented with 0 (control), 0.5, 1, 2, 5 and 10 mg/ml polyvinyl alcohol or polyethylene glycol then refrigerated for 24 hours. We found that only 0.5 mg/ml of PVA group had higher motility parameters comparing to control group (P <0.05). The lowest motility in PVA groups was observed at the concentration of 10 mg/ml. Extenders supplemented with PEG in different concentrations had no significant effect on the most kinetic indices. Both compounds were lethal to sperm at 15 mg/ml.

There are different activating salt solutions for sperm induction of rainbow trout (Oncorhynchus mykiss) under artificial spawning condition, although some uncertainties are associated with the performance. This study was conducted to consider the effects of different concentration of calcium and magnesium ions and change of pH in the Billard salt solution on sperm motility of rainbow trout. Four-year old broodstocks (n=10) of rainbow trout were randomly selected and adapted to the same experimental condition for two weeks. Experimental solutions were also prepared in triplicate by separately adding 1, 2, and 3 mmol calcium and magnesium to the Billard solution. Another three solutions were also prepared with pH adjustment from 9.0 in the Billard solution to 8.0, 8.5, and 9.5. The broodstocks were then transported to the hatchery and artificially spawned. The collected sperm from all broodstocks were mixed for eliminating the effect of genetically differences between the fishes. Finally, the rainbow trout sperm mobility in the experimental treatments was determined in triplicate and compared by the Billard solution as the control treatment. Sperm mobility of rainbow trout in all treatments was significantly more than the control (p<0.01). The maximum mobility with 105.0±6.7 s was obtained in the 1 mmol calcium treatment, while the lowest level was found in the Billard treatment (31.33±4.50 s). Between the experimental treatments, calcium had the most effect on the sperm mobility of rainbow trout, although no significant differences was observed on the sperm mobility between 3 mmol calcium and 3 mmol magnesium treatments (p<0.01). A downward trend in sperm mobility was found by increasing the pH of activating solutions with the highest and lowest sperm mobility in the pH of 8 and 9.5 treatments, respectively. The findings of the present study demonstrated that modification of activating salt solutions using each doubly charged ions of calcium and magnesium and the pH adjustment could improve the reproduction efficiency and hatching rate in the hatcheries of rainbow trout.