جستجوی مقالات مرتبط با کلیدواژه "تیپ a" در نشریات گروه "دامپزشکی"

This abattoir-based investigation was conducted in search for presence of Pasteurella multocida, collection of its isolates, characterization of their biochemical profiles and also classification of their capsular types in Tehran and Alborz provinces. Swabs from 89 lungs and 595 nasal passages of bovine calves at a single slaughterhouse in Karaj (Karaj city) and 5 slaughterhouses in Tehran (Shahriar, Shahr-e-Ray, Robat Karim, Kahrizak and Varamin) were cultured on specific media. Among the 339 non-haemolitic individual colonies, 89 were proved P. multocida by conventional biochemical tests. The PM-PCR assay confirmed identity of 64 isolates as Pm where 60 were shown to belong to the capsular type A and a single one to capsular type B. The remaining 3 isolates were non-typeable. We believe still a lot needs to be done at the national level to fully acknowledge the epidemiology of Pm in Iran.

Staphylococcus aureus is a major opportunistic pathogen causing various diseases such as pneumonia and urinary tract infections. Indiscriminate and excessive use of antibiotics leads to antibiotic resistance in this bacterium, especially against commonly used antibiotics in the treatment. The present study aimed to evaluate the antibiotic resistance pattern of Staphylococcus aureus strains isolated from milk and traditional milk products, especially resistance to methicillin. A total of 403 samples of milk and milk products was examined and collected from distribution centers of traditional and dairy products. Strains isolated were tested by PCR to determine the frequency of mec gene, SCCmec types and genes coding for antibiotic resistance. Antibiotic resistance pattern of isolates was assessed by disk diffusion method. Out of 403 samples, 151 samples (%44.67) were infected with Staphylococcus aureus, and the highest prevalence of infection was found in samples of cow raw milk (%58.55). Only IV type was detected in mecA-positive isolates among SCCmec types; a number of 66 isolates belonged to IVd type, 21 isolates related to IVc type and 13 isolates belonged to IVa type and the significant differences between SCCmec type IVd with two types IVc and IVa was observed. The high prevalence of infection with Staphylococcus aureus in raw milk and traditional milk products and high antibiotic resistance of the isolates to common types of antibiotics in treating human infections are serious warning to the community and it requires hygienic measures and quality control of dairy products more than ever.

Since, the most susceptible laboratory animal to Foot–and–Mouth disease virus (FMDV) is guinea pig, and then one milliliter of FMDV type (O) concentrations of 106-106.5 TCID50was inoculated intradermally (ID) to 10 plantar surface of guinea pig (right side). Guinea pig adapted virus has been prepared after generalization phase, and then one milliliter of virus was inoculated intradermally to 30 guinea pigs in 5 groups (each consist of six). Samples from different organs including, heart, lung, liver, spleen, pancreas, tongue and plantar epithelium, retropharyngeal and inguinal lymph nodes were collected on days 2, 4, 14, 30, 60 post inoculation (PI) and kept in transport media and sent to FMD department of Ref lab. According to the results of this study, plantar epithelium and tongue as well as lung have been detected to be the original sites for virus survival. In addition, the highest rate of the presence of virus can be seen on 4 days and 14 days PI. By the way there was no sign of presence of the virus in any organs on days 30 and 60 PI.

Rapid spreading of the low pathogenic avian influenza virus (AIV) caused by the H9N2 subtype and the highly pathogenic AIV caused by H5N1 have caused serious economic losses in the poultry industries of Asia. Therefore, the early detection of AIVs is crucial for the control of the disease. In the present study, the applicability of a rapid immunochromatographic (RIC) assay, which specifically detected type A antigens of AIVs, was evaluated. This assay detected H9N2 viruses at 103.2 ELD50/ml and H5, H7 and H9 antigens at 128 HA titers, but did not react with other respiratory viruses. The assessment of cloacal swab samples prepared from 1 to 10 d post-inoculation (PI) revealed that the first positive samples were detectable on day 2 and 3 PI, and the last positive samples were detectable on day 10 and 9 PI, by the virus isolation (VI) and RIC assays, respectively. Collectively, the relative specificity, sensitivity, positive predictive value, negative predictive value, accuracy and correlation rate of the RIC and VI assays, were 100%, 71.5%, 100%, 78.5%, 0.86, and 0.98, respectively. There was also a good correlation (? > 0.81) between the results of the haemagglutination (HI), VI and RIC assays of cloacal/tracheal swab samples that were obtained from broiler flocks involved with viral respiratory diseases. Overall, RIC showed a low sensitivity and high specificity for the rapid diagnosis of H9N2 isolates in both experimental and clinical infections.

Foot -and- mouth disease (FMD) is one of the most important virus disease in farm animals. Types O, A and Asia1 FMD virus have been endemic in Iran. In these study, samples from suspected livestock were analyzed by RT-PCR experiment. The number of 702 nucleotides determined at 1D -2B region of type A strain isolated from Khorasan Razavi province sequenced and compared with that of other reported isolates type A from Iran and neighboring countries.The results show that field isolated type A has about 89% similarity with other reported isolates type A from Iran and neighboring countries. Furthermore, this virus shows the most similarity with A/IRN/1/87(Samuel). Phylogenitic analysis revealed that virus was closely related to A22-Iraq/99 and A/IRN/iso/105 that rest in the same lineage. The data showed high similarity between type A viruses involved in the Khorasan Razavi province and A/IRN/87v (vaccine strain); so that it can be concluded that the vaccine can produce prophylactic antibody against this virus.