جستجوی مقالات مرتبط با کلیدواژه "سلول سرتولی" در نشریات گروه "دامپزشکی"

Ghrelin has recently emerged as pleiotropic regulator of a wide array of endocrine and non-endocrine functions. The former likely includes the control of gonadal function, as expression of ghrelin and its putative receptor, the GH secretagogue receptor type 1a (GHS-R1a), has been described in mammalian gonads, and direct effects of ghrelin in the control of testicular secretion and cell proliferation have been reported. Yet, the expression and/or functional role of ghrelin in gonads from non-mammalian species remain to be analyzed. This study investigated the immunohistochemical localization of ghrelin in testicular tissues of rooster, using mouse monoclonal anti ghrelin antibody as primary antibody and donkey anti rabbit IgG (HRP) Polyclonal antibody as secondary antibody. Samples of testis collected from five rooster (45 day olds) and for IHC test were kept in 10% Formalin. Then paraffin blocks and histological section for IHC test were prepared.Evaluation of the pattern of cellular expression of ghrelin protein in rooster testis using immunohistochemistry demonstrated that ghrelin peptide was located in the interstitial leydig, sertoli cells and germ cells. Thus, strong ghrelin immunostaining was observed in leydig cells, sertoli cells and germ cells of testicular tissue. No ghrelin signal was detected in control sections. In addition, the sheep testicular tissue was used as positive control Immunoreactions was observed in germ cells, Leydig and Sertoli cells. It is believed that site of ghrelin expression in germ cells, Leydig and Sertoli cells may indicate its role in local regulations. Results of this research are agree with our previous study on Holstein bull, researches on goat and sheep (unpublished data) and some other studies conducted on sheep, cattle and mouse. This is one of the first studies to provide molecular evidence for the presence of ghrelin within the entire testicular tissues of rooster.

The aim of the present study was to investigate the immunohistochemical localization of ghrelin in testicular tissues of male goat. Ghrelin, a 28-amino acid acylated peptide, has been identified as an endogenous ligand for GH secretagogue receptor and the role of ghrelin in reproduction has been recently established. The immunohistochemical localization of ghrelin in male goat testicular tissues was performed using mouse monoclonal anti-ghrelin antibody as primary antibody and donkey anti-rabbit IgG (HRP) Polyclonal antibody as secondary antibody. Samples of testis were collected from three male goats (1.5 to 2-years old), and were kept in 10% Formalin for IHC test. Then paraffin blocks and histological sections were prepared. Immunoreactions were observed in spermatogenesis process, Leydig and sertoli cells. It is believed that the site of ghrelin expression in stages of spermatogenesis, Leydig and sertoli cells may indicate its role in local regulations. This is one of the first studies to provide molecular evidence for the presence of ghrelin within the entire testicular tissues of male goat.

The aim of the present study was to evaluate gene expression of constitutive and inductive nitric oxide synthase enzymes (cNOS, iNOS) and measurement of nitric oxide metabolite in ovine sertoli cells under heat stress. Testes of ten male lambs were provided from abattoir and then transported to the lab for separating of sertoli cells. Heat stress was challenged in three groups which consist of control (temperature of 32˚C) group, mild heat stress (temperature of 39˚C) and severe heat stress (temperature of 42˚C). The amount of nitrite (nitric oxide metabolite) was significantly higher in 42 ˚C-group than control (p

The role of thyroid hormones has been recognized in normal embryo development many years ago. These hormones also affected the development of reproductive organs and their activity. This study was conducted to investigate the effect of inducing hypothyroidism by propyl-2-thiouracyl (PTU) on lambs testicular histomorpho-logy and plasma testosterone concentration. Eighteen Lori-Bakhtari male lambs were divided to 3 groups (n=6) and each received one of the treatments as Control (C: 0 mg PTU/kg BW), Low (L: 10 mg PTU/kg BW) and High (H: 20 mg PTU/kg BW) by gavages, during a 60d experimental period. At the end of the trial, lambs were slaughtered and testes were removed to evaluate their histomorphological characteristics. Mean concentration of T4 and T3 decreased significantly in L and H groups compared with C group (p<0.05). Hyphothyroidism increased testis weight, number of sertoli and lydig cells, diameter of Seminiferous tubules, diameter of seminiferous lumen, total spermatogonia, number of primary spermatocyte, and total spermatids (p<0.05). However, PTU had no significant effect on plasma concentration of testosterone (p>0.05). In the present study, PTU increased testicular weight and the number of cells involved in sperm and testosterone production. Sheep breeders may consider hypothyroidism as a potential approach to increase sperm production capacity in rams before puberty. However, further investigation should be carried out on the quality and quantity of ram's sperm.