جستجوی مقالات مرتبط با کلیدواژه « کلبسیلا پنومونیه » در نشریات گروه « دامپزشکی »

Klebsiella pneumoniae is a Gram-negative, non-motile, encapsulated, lactose-fermenting, facultatively anaerobic, rod-shaped bacterium.It has traditionally been considered an opportunistic pathogen and is a common cause of nosocomial infections. The pks gene cluster encodes enzymes responsible for the synthesis of colibactin. Colibactin is a genotoxin that has been shown to induce DNA damage and contribute to increased virulence. Colibactin is also strongly suspected of being involved in the development of colorectal cancer. The present study investigated the prevalence of pks, clbN, and clbB genes in Klebsiella pneumoniae isolates. In this study, 220 samples were obtained from raw milk. Then, all samples were cultured in the violet red bile agar and then cultured in blood agar, MacConkey agar, and chocolate agar for bacterial isolation. Biochemical and microbiological tests were performed for confirmation of the bacteria. DNA was extracted from all isolates using a genomic DNA extraction kit. Then multiplex polymerase chain reaction (M-PCR) method was performed using specific primers. It was found that 60 K. pneumonia isolates out of 220 samples (27.27%) were confirmed by standard phenotypic tests. The PCR test indicated that 6 (10%) strains carried clbN and clbB genes. The pks positive K. pneumoniae was more prevalent in our samples. Owing to its pleiotropic effects, colibactin profoundly influences cellular physiology, inducing DNA breaks that lead to senescence or apoptosis. It seems that the identification of the pks positive K. pneumoniae in milk is essential to prevent colorectal cancer.

Klebsiella pneumoniae is a gram-negative intestinal bacterium that causes nosocomial infections. The aim of this study was to evaluate the genotype of calcitonin-resistant genes (mcr-pmr) in Klebsiella pneumoniae isolated from raw milk by Multiplex-PCR. Samples were collected from 220 raw milk storage tanks in Tehran milk storage centers in 1977. Klebsiella pneumoniae strains were identified using standard biochemical and microbiological tests. DNA extraction was performed according to the National Center for Genetic and Biological Resources of Iran (MBK0041) kit. After PCR test in thermocycler apparatus to determine the presence of mcr and pmr genes, the samples were transferred onto 1% agarose gel and stained with ethidium bromide and then analyzed in dock gel apparatus. In this study, 60 genotypes of mcr and pmr genotypes were studied in 60 isolates of K. pneumoniae. Meanwhile, the Mcr gene was reported at a frequency of 11, (18.3%) and the Pmr gene was reported at a frequency of 7 (11.6). The trace of the two genes was investigated by Multiplex-PCR method. This seems to be the first study of two Mcr, Pmr genes, in Iran of milk samples isolated from milk storage tanks in industrial farms from Klebsiella pneumoniae and two of these genes were identified as the main marker.

Bovine mastitis is one of the epidemic diseases in dairy cattle, that it was created by various infectious agents. The aim of this study was tracking of these bacteria in the cases such as bovine subclinical and clinical mastitis and studying of virulence factors of this bacteria. Overall, 130milk samples were collected from dairy cattle's in the Chaharmahal and Bakhtiyari state scale that they are affected to mastitis and then they were isolated microbial culture and molecular confirmation of Klebsiella pneumonia strains, Presence of the most prevalent of virulence factors in these strains was detected by PCR method. Of 130milk samples examined, 30 samples were positive (15.38%) to Klebsiella pneumonia. Of these strains, the most of virulence factors were detected in this bacteria: so that fimH and papA genes by excess of 65and 90% had the highest prevalent and sfa/focDE gene with 15% presence had the lowest rate of evaluation virulence gene in this isolates. Presence of kinds of virulence factors in the Klebsiella pneumonia isolates will have indicated direct intervention these factors for bacterium pathogen and it is needed to detection of Klebsiella pneumonia pathogen, it must study presence distribution of virulence factors.

Klebsiella pneumoniae, is an opportunistic pathogens and cause infections in humans and animals. Drug resistant K. pneumoniae is rising. Therefore, antimicrobial susceptibility testing before prescribing antibiotics, it seems necessary. The aim of present study was to survey typing of clinical and animal K. pneumoniae isolates and evaluation of antibiotic susceptibility. A total 100 clinical and animal K. pneumoniae isolates were collected from Babak city. Antibiotic susceptibility was performed with Kirby-Bauer method according to CLSI guidelines. Then, DNA genomic extraction was done using DNA kit and PCR amplification was performed with ERIC1 and ERIC2 primer. Our results were shown that all strains (100%) were resistant to the ampicillin and amikacin antibiotics. The most and least resistance belong to tetracycline (53 strains; 88.3%) and imipenem (8 isolates; 13.3%), respectively. The results of cluster analysis and drawing dendrogram based on genetic similarities for 100 isolates was separated to seventeen distinct groups. According to our finding indicated an increasing resistance to antibiotics amongst K. pneumoniae. Additionally, the ERIC sequences have a pair of games that contain highly reversed and central reps and are located in the outermost regions of the bacterial genome and have less complexity in determining the genetic diversity of all isolates, but the separation good at the strain level.

A three month-old-male red deer calf (Cervus elaphus maral) was examined post mortem for the cause of death in Arasbaran preserved area in East Azerbaijan in September 2006. The main history of the case was the lack of colostrum intake after birth. The necropsy 6 hours after death, revealed severe general congestion especially in lungs and visceral organs (liver and jejunum). The cut surface of lungs was moist and bronchial lumina contained a large amount of frothy pinkish edema fluid. Diffuse congestion of lung and porteinous exudates was prominent in examination. Histopathological examination revealed shock lung and hepatocytes dissociation with single cell necrosis in liver. Microscopic examination was in line with shock lung and alveolar edema. No parasites were observed within red blood cells. Bacteriological cultures gave rise to gram negative cocoobacilli and further biochemical tests performed on isolated colonies revealed the presence of pure Escherichia coli in liver and Klebsiella pnemoniae in lung. Using serological tests, E. coli serotypes O20 and O114 and Klebsiella pnemoniae serotype K1 were identified in purified bacterial cultures. This report presents endotoxemia and death in a red deer calf with lack of colostrum intake and transportation stress history.