جستجوی مقالات مرتبط با کلیدواژه « amino acids » در نشریات گروه « دامپزشکی »

The most basic application of protein hydrolysis is nitrogen supply for bacterial culture media. Considering that about 200 million doses of Clostridium bacteria are used annually in our country and to produce this vaccine dose, 16 tons of hydrolyzed protein (peptone) is needed. Currently, this need is supplied through imports. Therefore, achieving the technical knowledge of peptone production from various inexpensive sources in the country is essential. Therefore, due to the development of vermicompost worm farms, inexpensiveness, availability, high protein in the body, hydrolysis protein of this animal was performed. Due to the development of vermicompost worm farms, inexpensiveness, availability, high protein in the body, protein hydrolysis of this animal was performed. Samples of worms were purchased from farms around Mashhad. For disposal of digestive system materials, worms were placed in a clean water and sand container for 24 hours. Then, by homogenizing them in water for 30 minutes, homogeneous suspension and uniformity of them were provided. Subsequently, chloroform was performed in two 30-minute times at 25c and 60c temperature, remove fat from protein tissue. Using six-normal hydrolytic hydrochloric acid vapor phase at 110c for 10 hours, protein hydrolysis was performed. Complete drying of the sample was completed in 3 hours under vacuum conditions with one bar pressure and 70°C. In this study, from the initial value of 10 gr of vermicomposting worms, three gr of hydrolyzed proteins were produced This amount of hydrolysis protein produced was 30% of the initial weight of worms.

The stratum corneum includes keratinocyte cells; this is the first defense layer which stands in the face of pathogenic microorganisms. Identifying the components of stratum corneum can provide plenty of information on its resistance against superficial infections. Stratum corneum contains higher amount of amino acids which most of them can serve as a source of nitrogen for the growth and colonization of dermathophytes. The objective behind this study is to investigate and compare the quantity of amino acids in dogs diagnosed with dermathophytosis and in healthy dogs. 30 dogs suspected lesions of dermatophytosis and 30 healthy dogs were used in this study. Skin scraping samples from 30 dogs with no obvious skin lesions and from 30 dogs suspected lesions of dermatophytosis were taken. Amino acids were analyzed by highperformance liquid chromatography in both groups. Between two groups, significant differences were found for the rate of aspartic acids, serine and asparagine amino acids. Aspartic acid seems to play inhibitory role in growth and colonization of dermathophytes in a way that less of it is found in infected dogs compared with healthy counterparts. It seems asparagine and serine has a stimulatory role in the growth and colonization of dermathophytes. The inhibitory properties of aspartic acids are suggested to be used in the manufacture of more effective antifungal agents especially for chronic type of dermatophytosis.

A study was conducted to determine the effects of diet formulation on Arian broiler breeder performance in 50 to 64 wk of age. The numbers of 168 female and 24 male breeders in 4 treatments with 6 replicates (7 females with a male) were used in the form of a randomized trial design 2 × 2 factorial. Four experimental diets were formulated base on two factors, first factor was included, two levels of Apparent and True Metabolisable Energy corrected for nitrogen (AMEn and TMEn), the second factor was included, two levels of Total Amino Acids (TAAF) and Digestible Amino Acids (DAAF) of Feed. The results showed that egg production, egg mass and feed conversion rate were significant difference on the main effect of energy (P<0.05). Broiler breeder fed TMEn was better and significant in egg production, egg mass and feed conversion rate (P<0.05). Percentage of eggs can incubate and hatchability was significant in the main effects of energy. Percentage of eggs can incubate was significant in interaction between energy and amino acids (P<0.05). Hatchability and number of chicks per hen was significant in the main effects of amino acids of feed (P<0.05). This experiment showed that the type of formulation of diets was significant effect on broiler breeder performance and diet formulation base on AMEN and DAAF for broiler breeder was better and significant performance.