جستجوی مقالات مرتبط با کلیدواژه « anaplasma » در نشریات گروه « دامپزشکی »

Babesia Starcovici 1893 and Theileria Theiler 1906 are the most important endemic blood parasites in domesticated animals. These parasites are transmitted by ticks and can causes clinical babesiosis and theileriosis both characterized by fever, hemolytic anemia which may lead to death. Therefore, the aim of our study is to determine the infection rates of Babesia spp. and Theileria spp. in Guilan province from Iran. A total number of 40 blood smears were collected from 40 different randomly selected cattle. The blood smears prepared and stained by Gimsa solution (10%) and light microscopic was used to examination of blood smears. The results showed that the number of 7 samples were infected by parasites. Among these, 3 samples (7.5%) were infected with Babesia spp. and 2 samples (5%) with Theileria spp. Along as 2 samples (5%) with bacterial parasites Anaplasma Theiler 1910. In the end, we recommend using both morphological and molecular methods together to get better results.

Anaplasmosis, babesiosis and theileriosis is parasitic diseases that can cause significant damage to the livestock industry. Despite the importance of the pathogenicity of theileriosis, babesiosis and anaplasmosis in large animal populations, in Iran, studies have been performed mainly on small ruminants. So, the aim of this study was to the diagnosis of the frequency of Theileria, Babesia and Annapalsma infections from cattle in Alborz province using microscopic examination and PCR (Polymerase Chain Reaction). For this purpose, out of 130 dairy cattle were taken blood samples and stained with Giemsa dye. Positive smear samples were incorporated for molecular detection. DNA was extracted from blood samples with phenol-chloroform and performed PCR using 18S rRNA, 16S rRNA and TamS primers to identify of the genus and species. The results of this study showed that frequency of piroplasmic form was observed in 9.23% of cases. The 18S rRNA PCR findings revealed 5.38%, were confirmed infection with Theileria and Babesia. Also, Tams1 PCR results were indicated Theileria anulata infection in all cases. 16S rRNA PCR was detected in 3.85% for Anaplasma. No case of Babesia was observed. Considering the cases of infection with T. anulata and Babesia and the importance of some species of Babesia, the importance of further studies to evaluate these diseases in order to improve the livestock industry seems necessary.

Blood parasites including Theileria, Babesia and Anaplasma are causative agents for theileriosis, babesiosis and anaplasmosis, respectively, which are responsible for tremendous economic loss to sheep farming industry worldwide. These parasites are mainly transmitted by hard ticks and carrier sheep are responsible for resistance of parasites in affected regions. Dezful city, south-west of Iran, has warm and humid climate and parasitic diseases including Haemoparasites seems to be prevalent among herds in this area. The purpose of this study was to evaluate prevalence of blood parasites in sheep of Dezful area using direct microscopy and PCR methods. For this reason, in August 2016, in a cluster sampling, a total of 200 blood samples from sheep of 4 regions of Dezful were collected randomly. Blood films were prepared and evaluated by microscopy. PCR was performed on 112 randomly selected samples. Prevalence of Babesia, Theileria and Anaplasma was 1.5%, 26% and 33% using microscopy, respectively while 72.72% and 0% of sheep were PCR positive for Theileria spp. and Babesia spp., respectively. No other blood parasite was observed in microscopy. The results of this study indicate high prevalence of Theileria and Anaplasma and low prevalence of Babesia among sheep of Dezful area. Regarding the results of this study, measures such as promoting knowledge of breeders regarding parasitic diseases of livestock, and in particular blood parasites and raising awareness of economic loss from these diseases through decrement in livestock production and increment in the costs of treatment, insecticide spraying of livestock places and bathing of animals against ecto-parasites are necessary for reduction of this parasitic infection.