جستجوی مقالات مرتبط با کلیدواژه "canine parvovirus" در نشریات گروه "دامپزشکی"

Canine parvovirus (CPV) has been incriminated as a primary pathogen related to acute hemorrhagic enteritis in dogs. Three major antigenic variants of CPV (CPV-2a/2b/2c) have so far been identified. This study was carried out to investigate the frequency of CPV-2 and its variants (CPV-2a/2b/2c) in a population of healthy and diarrheic dogs in the north west of Iran.
A total of 35 stool samples from healthy (n=16) and diarrheic (n=19) dogs were screened for all variants (2a, 2b, and 2c) by polymerase chain reaction (PCR) using primer pair 555for/555rev resulting in a PCR product of 583 bp in length. The resulting fragments were further digested by MboII endonuclease that selectively recognizes the restriction site “GAAGA” unique to CPV2c only. All undigested samples were subjected to PCR assays with primer pair Pab (which detects both CPV-2a and CPV-2b types) and primer pair Pb (which detect only CPV-2b type) primer pairs. The relationship of health status, breed, age, sex and vaccination status with PCR results were analyzed using statistical tests.
From a total of 35 samples, 10 samples were found to be positive by 555for/555rev primers that were further analyzed by MboII digestion of PCR products. One sample was characterized as CPV-2c and nine samples were categorized as CPV-2a or CPV-2b. All nine undigested samples resulted positive by PCR using Pab primers, out of which 7 resulted positive by PCR using Pb primer pairs, indicating that they are of CPV-2b variant.
It seems that CPV-2b is prevalent variant circulating in the North West of Iran. Results also indicated that CPV-2a and CPV-2c are affecting dogs, suggests constant surveillance and monitoring of CPV variants.

Canine parvovirus (CPV) infection is one of the most common causes of infectious gastroenteritis in dogs and is a highly contagious, often fatal disease. The original virus (CPV type 2) has had some mutations since its emergence and new variants (CPV-2a, 2b and 2c) have been reported from many countries all around the world. Early diagnosis and treatment can profoundly affect the disease outcome. To compare the ability of Immunochromatographic (IC) test to detect CPV infection in 50 PCR positive samples (n=50) with regard to virus strains. 50 rectal swabs (n=50) were prepared from suspicious dogs and subjected to PCR and IC test respectively. The sensitivity of IC test in PCR positive samples was 84% (42 out of 50 samples) and the positive predictive value of the test was 100%. Using PCR, CPV strains in our study were 2a (18/50, 36%) and 2b (32/50, 64%) with the predominance of 2b strain. IC test was also able to diagnose 15/18 (83.3%) of CPV- 2a and 27/32 (84.3%) CPV-2b strain positive samples, which means IC test can detect CPV infections caused by both virus strains (2a and 2b), without significant difference. This study shows that IC test results are relatively reliable for diagnosing CPVinfection in daily veterinary practice and the test is able to diagnose both CPV-2a and CPV-2b which are prevalent strains in Iran.