جستجوی مقالات مرتبط با کلیدواژه « mrna » در نشریات گروه « دامپزشکی »

The compound 1-methyltryptophan (1-MT) has been shown to act protectively in renal ischemia-reperfusion injury. Toll-like receptor 4 (TLR-4) signaling is also a regular process of epithelial to mesenchymal transition (EMT) that can after Ischemia-reperfusion injury (IRI) result in as an increase of renal fibrosis. EMT is associated with specific transcription factors – Snai1, Snai2, Zeb1 and Twist. 1-MT could regulate EMT and act as antifibrotic agent. This study aimed to investigate the effect of 1-MT on EMT transcription factors in tubular epithelial cells that underwent 30 min. Renal tubular epithelial cells (TECs) were isolated from Lewis rats using a standard protocol with Fe2O3 magnetic separation and selective media as previously mentioned. ischemia and 48 hours of reperfusion. Cells were cultivated and divided into 4 groups: C-TECs– control cells, IRI-TECs – IRI-induced TECs, D-IRI-TECs – IRI-induced TECs treated with 1-methyl-D-tryptophan, L-IRI-TECs – IRI-induced TECs treated with 1-methyl-L-tryptophan. IRI was induced in all groups for 30 min by mineral oil (except for C-TECs) followed by 48 hours of reperfusion. RNA and proteins were isolated from harvested cells. Using semi-quantitative PCR (RT-sqPCR) we assessed the relative mRNA expression of EMT transcription factors Snai1, Snai2, Zeb1, and Twist. Hereby we have shown, that the treatment of ischemia-induced TECs with both 1-MT isomers lowers the expression of EMT transcription factors Snai1 and Zeb1 that were increased by ischemia and reperfusion of TECs. This could act favorably in renal IRI decreasing EMT and renal fibrosis, therefore showing the potential of 1-MT as a part of therapy in renal transplantation aimed at renal ischemia-reperfusion injury.

Leptin is secreted mainly by fat، which is involved in energy metabolism and reproduction. Leptin and its receptor (Ob-R) have been detected in human spermatozoa and testis، thus it can be concluded leptin involves in male physiology. The goal of this study was determination of the presence of leptin receptor mRNA in the ram testis، epididymis and spermatozoa by reverse transcription polymerase chain reaction (RT-PCR). Ejaculated spermatozoa from ten fertile Taleshi ram were collected by means of an artificial vagina. Testes، placental cotyledons and adrenal glands were obtained from abattoir. Placental cotyledons and adrenal glands were used as the positive control. Epididymal spermatozoa recovery was performed from epididymis. To purify spermatozoa، motile sperm were isolated by the swim-up procedure. Total RNA was isolated from epididymal spermatozoa، ejaculated spermatozoa، adrenal gland and placental cotyledon and then they were purified. The mRNA for the long form (Ob-Rb) and the short form (Ob-Ra) of leptin receptor was detected in testis. RT-PCR analysis of total RNA from epididymal spermatozoa and ejaculated spermatozoa revealed the presence of leptin mRNA in these cells. The mRNA for Ob-Rb was observed in epididymis and epididymal spermatozoa، but the Ob-Ra mRNA was absent. The presence of Ob-Ra mRNA was found in ejaculated spermatozoa، whereas Ob-Rb mRNA did not exist. It can be concluded that the mRNA for leptin receptor is present in ram gonads and spermatozoa.