جستجوی مقالات مرتبط با کلیدواژه « testis » در نشریات گروه « دامپزشکی »

Chemical castration is a method of castration by medication. The effect of injecting 20% calcium chloride solution into dog testicle tissue was done with the help of ultrasonography, histopathology, and blood parameters. Several 18 healthy male dogs were selected and divided into three groups: surgery, ultrasound, and histopathology (number=6). On days 0, 7, 14, and 21, ultrasound (length, width, depth, and echogenic) and blood sampling were performed. On day zero, 20% calcium chloride was injected into the testicular tissue of the ultrasonography and histopathology groups. Oxidative stress enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPx) and blood testosterone were measured on days 0, 7, 14, and 21. Histopathology was examined on the 7th and 14th days of sampling and by hematoxylin and eosin staining. Ultrasonography showed a statistically significant difference between the average length, width, depth, and echogenicity of both groups except on day zero. In addition, the echogenicity of the left and right testicles was significantly (p<0.0001) reduced in the studied groups. The testicular tissue suffered from thrombosis, necrosis, leukocyte infiltration, calcification, and granulomatous masses. The oxidative stress enzymes on day 21 were significantly (p<0.0001) lower than on day 0. However, the testosterone level had not changed even on the 21st day. Although the chemical method of calcium chloride is effective and cheap, with few side effects and without anesthesia, the effects of this method are slow. On the other hand, it leads to oxidative stress reactions in the animal. However, it is a more appropriate method.

Repeated exposure to iron oxide nanoparticles can lead to structural disorders in the testicles and have negative effects on the individual's reproductive function. On the other hand, spirulina algae can reduce these effects by having antioxidant properties and abundant vitamin source. The purpose of this study was to investigate the effects of spirulina algae on tissue changes in the testes of rats induced using iron oxide nanoparticles. After acclimatization, 36 Wistar male rats were randomly divided into the following 6 groups. The first group: control, the second group: 15 mg/kg body weight of iron oxide nanoparticles, the third group: 300 mg/kg body weight of spirulina algae, the fourth group: 15 mg/kg body weight of iron oxide nanoparticles and 300 mg/kg algae body weight. spirulina at the same time, fifth group: 15 mg/kg of body weight of iron oxide nanoparticles for 14 days, then 300 mg/kg of spirulina algae, sixth group: 300 mg/kg of body weight of spirulina algae for 14 days, then 15 mg/kg of body weight of iron oxide nanoparticles. Sampling was done after 60 days and testicular bud sections were stained by hematoxylin-eosin (H & E) after preparation for histological and histometric studies. The study of tissue sections of the testis in rats exposed to iron oxide nanoparticles showed a decrease in the diameter of the spermatogenic tubules and the thickness of the epithelium of the spermatogenic tubules, a decrease in the number of Sertoli cells and the number of Leydig cells in comparison with the control group, while exposure to spirulina algae increased the diameter of the spermatogenic tubules and the thickness of the epithelium of the spermatogenic tubules. Algae treatment in the groups exposed to iron oxide nanoparticles improved the tissue changes in the testis and prevented the decrease in the diameter of the spermatogenic tubes, the thickness of the germinal epithelium, and the number of Leydig and Sertoli cells. The results showed that the oral administration of spirulina in male rats improves the structural disorders in the testis and tissue changes caused by induction by iron oxide nanoparticles.

Air pollution in different cities of the world, has become a one of the most important environmental problems, which the effect of its on human health, especially fertility, has been considered. This study aims to investigate the impact of PM2/5 and gas pollution on various aspects including the, sex hormone changes, sperm DNA fragmentation and also (MS-HRM) assay of aquaporin 9 gene in testis tissue. After sampling and evaluating the concentration of PM2/5 24 Wistar rats (weight 170 to 200 g ) were used. The animals were randomly divided into three equal groups including the exposure group 1 (received PM2/5 and gaseous pollutants), exposure group 2 (received gaseous pollutants), and the control group (received the air filtered). After 6 months the animals were investigated for hormonal changes such as FSH, testosterone as well as sperm DNA fragmentation and also methylation-sensitive high-resolution melting (MS-HRM) assay was performed. The data obtained showed that the serum level of FSH and testosterone are significantly increased and decreased in the exposure group 1 compared to the control group, respectively. The exposure group 2 also significantly showed lower the testosterone level than that of the control group. A significant difference was also seen in both exposure groups 1 and 2 with the control group respected to sperm DNA fragmentation. MS-HRM assays detected only hypermethylation of Exposure 1 in comparison with control group in a period of six months. Therefore, exposure to PM2/5 and gas pollutants leads to interference in the secretion of sex hormones and has a detrimental effect on sperm DNA fragmentation, resulting in disruption of male reproductive function, which is crucial in inducing infertility in males. These findings may help in identifying therapeutic targets for reducing human infertility disorders caused by exposure to PM2/5.

Administrating antineoplastic and alkylating drugs such as busulfan can cause subfertility. The purpose of this study was to evaluate changes in testicular tissue, serum testosterone and anti-Müllerian hormone levels following busulfan administration in mice.

Thirty-two adult male mice of the Balb/C strain were randomly divided into two groups (n=16), control and busulfan. The animals of the control group did not receive drug treatment, but the animals of the busulfan group received two doses of busulfan (10 mg/kg body weight) at an interval of 21 days. 35 days after the second injection, the animals of the busulfan and control groups were anesthetized and blood was taken to measure testosterone and antimullerin hormones. The testicular tissue was also removed for histopathological evaluation. Hormonal data were analyzed using one-way variance test and LSD post hoc test.

Hormonal data showed that the serum levels of testosterone and antimullerin hormone in the busulfan group were not significantly different from the control group (P>0.05). Also, the histopathological evaluation of the testicular tissue in the control group showed that the seminiferous tubules have a regular structure and spermatogenesis is normal, but in the busulfan group, the lumen space in the seminiferous tubules was wide, the thickness of the germinal epithelium was lost, and spermatogenesis was completely destroyed.

Administration of busulfan with two doses of 10 mg/kg has no effect on the serum levels of testosterone and antimullerin hormone, but it can cause testicular tissue destruction and azoospermia induction in mice.

In this study, the effect of L-Carnitine was investigated on reducing the alcohol damage in the testes of adult rats. Alcohol leads to oxidative damage and L-Carnitine protects from oxidative damage as an antioxidant. In this study, in 20 adult male Wistar rats damage were induced by 30% ethanol with a dose of three gr/kg. To prevent injury, L-Carnitine with a dose of 100mg/kg was used. Finally, blood samples were taken from the rats and testosterone concentration was measured. After the rats euthanizing, the testes were removed, weighed and fixed in 10% formalin buffer solution for 72 hours. After processing with standard histological methods and paraffin molding, 5 microns sections were prepared and studied histomorphometrically after H&E staining. Findings of this study revealed that Ethanol significantly reduced testicular weight, serum testosterone concentration, and testicular histomorphometric parameters. Treatment of L-Carnitine resulted in a significant increase in testicular weight, seminiferous tubule diameter, epithelial thickness, serum testosterone concentration and number of Spermatogonia, Primary Spermatocytes, Spermatids and Leydig cell compared to the positive group. Therefore, it can be concluded that oral L-Carnitine at a dose of 100 mg/kg can partially prevent the damage caused by ethanol.

Testicular ultrasonography is a standard method for examination in animals. Considering that ultrasound is a valuable and non-invasive method to evaluate the morphology and health of the reproductive system in veterinary medicine, therefore, in this study, the relationship between the ultrasound characteristics of the testis and epididymis with the quality of semen in Saanen male goats was investigated. Also, the minimum age that Saanen male goats can produce qualified sperm for fertility was evaluated.

Twenty-four Saanen male goats aged 6 to 18 months were studied. Measurement of the scrotum circumference and ultrasound of the testicle and epididymis were performed and the size of the scrotum circumference, length, width and volume of the tail of the epididymis were obtained. Then, semen was obtained by electrical stimulation method and sperm parameters were measured.

Mean body weight, scrotal circumference, length, width and volume of epididymis were 32.99±4.01 kg, 220.96±0.24 mm, 1.77±0.14 mm, 1.91±0.14 mm and 5.43±0.43 mL respectively. Also, the results of the quality of semen, state that the average of semen volume, number of sperm, percentage of live, motile and normal sperm were 1.66±0.17 ml, 22.59×106±1522, 71.40±2.34%, 57.51±2.04% and 67.65±3.13%, respectively.

All ultrasonographic parameters increased with increasing age, and the standard values were determined at 6, 12, and 18 months of age. According to the results of semen parameters, it can be concluded that Saanen goats at the age of 18 months have acceptable parameters for fertility. Therefore, it is recommended to use Saanen goat sperm from at least 18 months of age to obtain better results in the fertility of this breed.

The testis is an important component of male reproductive system and is responsible for spermatogenesis The objective of the present study was to determine the prevalence of testicular pathology in rams and bucks in Fars province, south of Iran. An abattoir-based survey was conducted between winter 2018 and winter 2019 in Shiraz and Kazeroun industrial slaughterhouse. The bucks were categorized into three age groups of <2, 2–3, and <3years before slaughter using dental examination After slaughter, the whole genitalia were examined for evidence of gross pathological abnormalities. Tissue samples from testis were collected and fixed in 10% buffered formalin, processed, sectioned, and stained with hematoxylin and eosin for histopathological examination using light microscopy. Data obtained were summarized and presented as number of occurrencesand proportion of total population examined Out of 200 rams and 200 bucks examined in the incidence of testicular lesions was 39 (19.5 %) and 58 (%29) respectively. Testicular mineralization, besnoitiosis, testicular atrophy, testicular hypoplasia, cryptorchidism and orcitis accounted for incidences of 21 (10.5%), 11 (5.5%), 10 (5%), 8 (4%),6 (3%) and 2(1%) respectively in bucks. Testicular mineralization 10 (%5), testicular hypoplasia 8 (%4). testicular atrophy 13 (6.5%), cryptorchidism 7 (%3.5) and orcitis (%1) were the pathological conditions found in rams.