جستجوی مقالات مرتبط با کلیدواژه « MTT » در نشریات گروه « دامپزشکی »

Leishmaniasis is a protozoan zoonosis that causes problems in public health. The use of pentavalent antimony compounds as chemical drugs in the treatment of cutaneous leishmaniasis are associated with various limitations and side effects.Today, the plant extracts and their derivations are the promising sources in herbal medicine. The purpose of this study was to evaluate the effectiveness of oil extract Pistacia atlantica from Uramanat area in Kurdistan province, Iran, on promastigotes of standard strain Leishmania major in vitro. In this study, 10, 15, 25, and 50% concentrations of oily extract were added to the plate containing promastigotes of Leishmania major. The effectiveness of different concentrations of extract was determined by MTT (3-[4, 5- (dimethylthiazole-2-yl]-2,5-diphenyltetrezolium bromide) colorimetric method and counting of living promastigotes. The results of present study showed, different concentrations of oil extract has an inhibitory effect on promastigotes of Leishmania and the extract 50% has the most effect. The results showed a significant different between  in the number of promastigotes of Leishmania major in different concentrations of extract and exposure times (24, 48, and 72 hours) (P≤0.05). The results show that extract of pistacia atlantica has an appropriate effect on Leishmania major in vitro. Therefore, it is suggested to  be evaluated the effect of the extract Pistacia atlantica on leishmaniasis wounds.

Neospora caninum, the cause of Neosporosis, is an obligate intracellular parasite. Neosporosis infection has worldwide distribution and infect many animals such as cattle, sheep, goat and deer. The control methods of disease are included test and cull, antibiotic treatment and vaccination. The lack of proper drugs and the absence of effective vaccines led us to evaluate the anti neospora effects of Ginger and Glycyrrhizin aqua extracts. In this study, different doses of two herbal extracts were prepared. At first the cytotoxicity of aqua extracts was evaluated on Vero cell line by MTT assay. Then their effects on parasite were tested in chicken embryonated eggs. The results showed that Ginger and Glycyrrhizin aqua extract, have no cytotoxic effect on Vero cell line in 0.625 mg/ml dose. Also, in the study on embryonic eggs, there were casualties in the groups that used clindamycin and Glycyrrhizin,while the ginger prevents losses due to the neosporosis in chicken embryonated eggs. Previous reports had proved the effect of ginger on Toxoplasma gondii .In this study, the inhibitory effect of this plant was also shown on Neospora caninum. In addition, for the first time embryonated eggs were used to evaluate drug effects on Neospora caninum. Both of these findings can be useful in applied research for the preparation of an effective drug for the control and treatment of neosporosis.

Lumpy skin disease virus (LSD), Goatpox virus and Sheeppox virus are members of genus Capripoxvirus of the Poxviridae, which have close genetic similarity. Therefore, the use of CaPV-vaccine strains derived from sheep and goat would be useful to protect cattle against LSD. The aim ofthis study was to evaluate the immune responses characteristics of available goat pox vaccine against LSD in cattle. All immunized calves were daily examined for any increase of rectal temperature and appearance of adverse reaction for 5 weeks following vaccination. Humoral immune responses were evaluated by serum neutralization test and cellular immune response were evaluated by MTT, Real-time PCR and ELISA tests. The results showed that theFever and localized swelling at the injection site were observed in vaccinated calves, in comparison to the control group. In assessing of humoral immune response, a significant increase in the neutralizing antibody titer was observed in the vaccinated group in comparison to control group, although this difference in days 35 was higher than the other days (p<0.05). In assessing the cellular immune response, a significant increase was shown for IFN-γ gene expression on day 7 and for IL-4 gene expression on day 21 postvaccination in comparison to day 0 and control group (P<0.05), While the highest cytokine production in cell culture supernatant for IFN-γ and IL-4 was observed at day 21 postvaccination (P<0.05). Also, the lymphocyte proliferation index in vaccinated calves showed a significant increase on day 7, 21, and 35 in comparison to day 0 and control group (P<0.05). According to the results of this study, the results of this study indicated, that goat pox-vaccine due to induction of high level of antibody titer, higher lymphocyte proliferation and IFN-γ and IL-4 production has a good immunogenic response, So It considered suitable vaccine to control of LSD.

Cutaneous Leishmaniasis is an endemic disease in some parts of Iran and parasites disease without an effective medical treatment.  In this study we attempted to evaluate the antileishmanial effects of the black tea decoction extract as herbal medicines. L. major promastigotes were cultured in RPMI-1640 medium enriched with 10% FCS in the presence of 10, 20, 30 and 40% of extracts. Parasites behavioral reaction and growth pattern was monitored after 0.5, 1, 5, 24, 48 and 72 hours post-seeding by counting fixed parasites in a haemocytometer chamber and observing their motility pattern and shape by invert microscopy. Parasite viability was evaluated by MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assay.  The results indicated that the number of promastigotes in groups containing 10, 20, 30 and 40% extract had decreased after 24, 28 and 72 hours while normal growth was observed in the control group which was significantly different (p<0.05) in comparison with the treatment groups. This study indicated that black tea decoction extracts could effectively prevent the growth of L. major promastigotes in vitro.