جستجوی مقالات مرتبط با کلیدواژه « Sperm » در نشریات گروه « دامپزشکی »

Despite the significant progress in sperm cryopreservation methods in recent years, research has proven that long-term storage of sperm in freezing and cold storage causes serious damage to sperm. The occurrence of fat peroxidation and the metabolism of spermatozoids results in destruction of spermatozoa during freezing and storage. Temperature shock, the formation of intracellular ice crystals, cellular dehydration, increased salt concentration and osmotic shock may occur during the freezing and thawing process. In addition, cryopreservation causes harmful structural changes in the plasma membrane. Changes in membrane permeability to some ions such as calcium during the freezing and thawing process have been reported. Also, during cryopreservation, the formation of intracellular and extracellular ice causes cell destruction and death. The sperm plasma membrane is the main site of damage during the freezing and thawing process and is very sensitive to lipid peroxidation due to the presence of many unsaturated fatty acids. These changes may have a role in the accumulation of toxic products of metabolism, mainly reactive oxygen species (ROS) that are generated through lipid peroxidation of sperm membranes. Small amounts of ROS play an important role in sperm capacitation, increased sperm motility, acrosome reaction, and oocyte fertilization in humans, cows, horses, pigs, and rams. Therefore, to solve these problems, various antioxidants, preservatives and chelators are used in sperm extenders.

The maltose disaccharide is easily converted into two glucose molecules during cellular glycolysis and is an energy source for sperm motility. To evaluate the effect of maltose on the kinetics of water buffalo epididymal sperm, 10 pairs of buffalo bull testicles, after the usual industrial slaughter, were transported to the laboratory of the Faculty of Veterinary Medicine in a polystyrene box at a temperature of 5o to 8o Celsius. In the laboratory, after fixing the tail of the epididymis with two fingers, several incisions were made in noncapillary zones. After the milky liquid containing concentrated spermatozoa was extracted, this liquid was transferred to an Eppendorf cell culture medium containing 10% bovine serum albumin (BSA). Five levels of maltose sugar (1, 3, 5, 10, 15 mM) were added to the Eppendorf containing 1 ml culture medium and 30-40 million sperm with 10% BSA and incubated at 37o C for 24 hours. Sperm kinetics were assessed at 1, 6, 12 and 24 hours by computer-assisted sperm analysis (CASA). Statistical analysis showed that CASA data such as rapid progressive motility (class A, %), progressive motility (class B, %), viability (class A+B+C, %), straight-line velocity (VSL, µm/s), curvilinear velocity (VCL, µm/s), average path velocity (VAP, µm/s) and lateral head amplitude (ALH, µm) at 24 hrs were higher in the maltose group than in the control group (p<0.05). In conclusion, this study suggests that maltose has a desirable effect on buffalo epididymal spermatozoa and maltose may act as an energy substrate for sperm motility.

Medicinal Plants with antioxidant properties can improve the quantitative and qualitative specific indices of sperm fertility and increase hatchability rate in rooster by disturbing the production process of free radicals and neutralizing of oxidative stress. The aim of this study was to investigate the effect of various concentrations of ginger extract Specific parameters of sperm in roster. In this study, hydroalcoholic extracts of knotgrass was prepared in Golpaygani roosters. In this study, hydro-alcoholic extract of ginger plant was prepared at concentrations of 0, 500, 1000 and 2000 mg/L and was added to drinking water of 36 adult male (32 weeks old) Golpaygani cock. After two week, the effect of different concentrations of the extract on the specific indices of sperm fertility (SMI, FSC, PMSCa, PMSCb and MSC) were evaluated and compared. The papenicular staining was also used to specific evaluation of sperm abnormalities. The use of ginger extract significantly improved the sperm fertility indices. The most number of sperm with fast progressive movements (PMSCa), the highest levels of SMI, FSC and MSC indices, the least structural and motility abnormalities was demonstrated in 1000 and 2000 mg extract (P≤0.05). Significant differences were not observed between the two above-mentioned intermediate and high concentrations in some parameters (P> 0.05). There was no significant difference in above indices between medium and high concentrations of extract . In conclusion, ginger , due to the high amounts of antioxidant compounds, significantly increases the sperm indices and also improves the specific parameters of sperm fertility in rooster.

The aim of this study was to evaluate the sperm quality preservation in sheep using mitochondria-targeted antioxidant Mito-TEMPO during chilling process. In this experiment, semen samples were collected from five Zandi rams and added to the extender containing 0, 0.5, 1, 5, 50 and 500 µM Mito-TEMPO and stored at 4 ˚C until 48 hours. Sperm quality was evaluated at 0, 24 and 48 hours after chilling and total motility, progressive motility, viability, membrane integrity, mitochondrial activity and lipid peroxidation were assessed. No difference was observed between treatments at time 0 of cooling. During 24 and 48 hours cooling periods, using 5 and 50 µM Mito-TEMPO increased total motility, progressive motility, viability, membrane integrity, mitochondrial activity and decreased lipid peroxidation in ram chilled sperm. In conclusion, using 5 and 50 µM Mito-TEMPO in chilling herbal extender could be a suitable method to conserve ram sperm quality during chilling process.

The ability of sperm to perform fertilization depends on various characteristics such as movement, viability, and sufficient volume. The present study was conducted to investigate the effect of Thymus vulgaris essential oil on the motility characteristics of ram sperm using phase-contrast microscope and CASA (Computer Assisted Sperm Analyzer) software. For this purpose, semen was collected twice a week from four rams. Initial evaluations included volume, wave motility, progressive motility, non-progressive motility and survival percentage of sperms. Then sperm samples were mixed with standard index Tris-based diluent, 20% egg yolk, 7% glycerol, plus 0, 100, 200 and 400 µg of Thymus vulgaris essential oil per ml. After bringing the temperature of the sperm samples to 5 degrees Celsius in the refrigerator, they were placed at 4 cm above liquid nitrogen for 10 minutes and finally immersed in it. The samples were thawed on days 0 and 30 of the experiment. Traits which were investigated during freezing and thawing days included total and progressive motility, non-progressive motility, viability percentage, MDA (Malondialdehyde) and CASA characteristics. The results showed that the percentage of viability and mobility decreased significantly during the experiment (p<0.001). The treatments of 100, 200 and 400 µg Thymus vulgaris essential oil had the most significant percentages compared to the control group in the characteristics of progressive and total motility, viability and CASA (p<0.001). Malondialdehyde had significantly decreased in the 100 and 200 treatments compared to the 400 and control treatments (p<0.001). The results of the present study showed that diluents containing Thymus vulgaris essential oil improve sperm motility and viability during freezing.

Varicocele is a pathological dilation of the venous network of the spermatic cord and considering that magnesium oxide nanoparticles play a key role in various physiological functions, the aim of this study was to evaluate the performance of nanoparticles on sperm characteristics affected by experimental varicocele. A total of 54 male Wistar rats were randomly divided into 9 equal groups including healthy control group (untouched animals), sham-operated group (underwent sham surgery), three healthy experimental groups (animals in these groups received magnesium oxide nanoparticles at doses of 1.25, 2.5 and 5 mg/kg respectively by gavage for 6 weeks), varicocele control group (varicocele was induced by renal vein ligation) and three experimental varicocele groups (in addition to varicocele induction, magnesium oxide nanoparticles were given at doses of 1.25, 2.5 and 5 mg/kg respectively by gavage for 6 weeks). At the end of the 6th week, the abdomen was opened and semen samples were collected from the tail of the epididymis to determine the indices of concentration, survival and motility of sperm and the data were statistically analyzed (p<0.05). The results showed that magnesium oxide nanoparticles were able to significantly increase the concentration, viability, progressive, moderate and non-progressive movements of sperm compared to the varicocele group (p<0.001) and also caused a significant decrease (p<0.001) in the number of non-moving sperms in the varicocele experimental groups. Therefore, magnesium oxide nanoparticles may possibly reduce the destructive effects of varicocele due to their antioxidant activity and be effective in its treatment by improving sperm properties during varicocele.

Bovine Herpes Virus-1 (BHV-1) belongs to the  Alpha herpesviral family. The virus is the cause of Infectious Bovine Rhinotracheitis (IBR) and Bovine Abortion. In the initial infection, the virus proliferates excessively. Moreover, shedding the virus leads to conditions in the latent phase of the disease. Infectious Bovine Vulvovaginit (IPV ) is the genital form of the disease that represents a genital infection and transmits via pustules and mucopurulent secretions. Exposure to the virus in genital mucosa leads to IPV infection through mating or artificial insemination and the diseases that can be transmitted to healthy livestock by frozen sperm during artificial insemination.

Viral contamination of the semen is one of the routes to spread the disease among dairy cattle. Therefore, we investigated the presence of the virus in domestic and frozen imported semen consumed in industrial dairy cattle farms.

In the present study, 140 frozen straws were collected. After melting each straw, 200 µl of obtained semen was used for DNA extraction, which was done directly on the semen samples and via a Genome Extraction Kit. Subsequently, to ensure the accuracy of the extraction, the PCR technique was done using PRM-1 gene primer. Tracking the viral genome was done using the PCR technique and known primers.

In total, one out of 140 samples was found to be virally contaminated, and IBR contamination was confirmed by repeating all the steps and determining the gene sequence.

It is necessary to further investigate the possibility that contamination can be transmitted via frozen semen, given that even one out of 140 samples is contaminated, and the importance of the disease.

Storing semen at a temperature of 4 degrees Celsius reduces its motility and quality.

This study aimed to determine the effect of adding calcium compounds, including calcimaphor (CMP), calcium gluconate (CG), and calcium chloride (CaCl2), on the motility and progressive motility of rooster sperm kept at refrigerator temperature.

This research used five pieces of 45-week-old Lari breed roosters. The liquid diluent added different calcium compounds at 0.56, 0.056, and 0.0056 mM concentrations. After treatment, the diluted seminal samples were cooled at the storage temperature to avoid thermal doubt and then transferred to the refrigerator at 4 degrees Celsius. Parametric factors that were more important such as the percentage of laterality and progressive mobility, were measured visually using the lens of a 40-light microscope, survival was checked using the eosin-nigrosin staining method, acrosome health percentage by formalin citrate method, cell membrane health with hypoosmotic test, lipid peroxidation level, fertility was evaluated using perivitelline membrane sperm reaction 72 hours after storing at 4 degrees Celsius.

Based on the results, different calcium compounds in most concentrations could significantly affect the parameters of survival, mobility, and progressive mobility (P<0.05).

Most of the sperm-related parameters in the control group decreased after this period of storage in the refrigerator, but the addition of calcium gluconate (0.56, 0.056, and 0.0056), Calcimaphor (0.56, 0.056, and 0.0056) and calcium chloride (0.56, 0.056 and 0.0056) to the semen thinner maintained the quality indicators of rooster sperm.

The aim of this study was to investigate the effect of diet supplementation with bindii (Tribulus Terrestris), carob (Ceratonia siliqua) and ginger (Zingiber Officinale) on semen quality and fertility potential of rooster breeders. In this experiment, 30 roosters were assigned into 6 equal groups and fed the following diets during 30 days: 1) the base diet, 2) diet containing bindii, 3) diet containing carob, 4) diet containing ginger, 5) diet containing bindii and ginger, 6) diet containing bindii and carob. Semen samples were collected via abdominal massage once a week and the following parameters were evaluated: semen volume, number of spermatozoa, sperm motility, membrane integrity, morphology, viability and fertility potential. In result, diet supplementation with herbal additives in treatments received groups improved sperm quality and fertility potential compared to control group (P≤0.05). In conclusion, using herbal additives in rooster diet could be an effective method to improve rooster sperm quality and fertility potential.