Comparision of antifungal effect of fluconazole alone and in combination with nanosilver particles against candida species isolated from chronic candidal vulvovaginitis

Candidal vulvovaginitis is a female genital infection that is occured by the over growth of candida species and specially Candida - albicans and occasionally it appears as recurrent and chronic and resist to therapy. Fluconazole is one of the current drugs that is used in treatment of this disease and sometimes resistance is observed to this. Therefore indirection of making the therapy better we decided to investigate the activitiy of fluconazole in combination with silver nanoparticles against candida species isolated from chronic and recurrent candidal vaginitis. This was an experimental study with convenient sampling that was performed on 30 patients. All specimens were examined in direct microscopy, culturingand differential tests to identify different candida species from each other such as culture on candida chrom agar, germ tube, temperature test and sugar assimilation with API. Then the antifungal effects of fluconazole and silver nanoparticles, each of them alone and in combination with each other, were examined. Findings were described on the base of logestic regression and man-vitni exam. In our study 30 specimens of chronic genital candidiasis were diagnosed with isolated agents Candida albicans, glabrata, krusei, tropicalis, parapsilosis and Candida famata respectively. Also findings suggested that fluconazole was able to inhibit the growth of candida species at an expanded range of concentration between 4-128 microgeram per milliliter.As well antifungal activity of Fluconazole with silver nanoparticles was increased in comparision with using Fluconazole alone. For the prevention of recurrent cases and to stabilish correct diagnosis it is essential to carry out sensitivity and diagnostic tests in laboratory and also administration of silver nanoparticle in combination with fluconazole in drug formulation for topical uses in treatment of chronic vaginal candidiasis and inhibition of recurrent cases, can useful.