Developmental Studies of Seminiferous Tubules Structure of Buffalo Testis

The sexual maturity is a major factor of fertility and reproduction activity. Since no information was availabe about microscopic developement of seminiferous tubules structure including spermatogenic cells, sertoli, PGC (Primordial Germ Cell) and laydig cells, the present detailed study on buffalo testis was undertaken. The present study was made on 30 buffaloes testes, divided into four groups Viz. 1) 3.5-6 months 2) 7-11 months 3) 12-23 months 4) 24-36 months. Tissue samples were processed for paraffin section and stained by Haematoxylin and Eosin stains. The microscopic observation showed that the seminiferous tubules had tubular structure with a define lumen at 3.5 months of age and their epithelium was consisted of abundant spermatogonia, PGC and sertoli cells. The primary and secondary spermatocyte, spermatid and spermatozoa were not seen upto 9 months of age. The coiling and thickness of seminiferous tubules were increased at one year of age. The leydig cells were seen as large clusters in interstitial connective tissue, PGC, sertoli cells, spermatogonia, primary and secondary spermatocyte, spermatid and spermatozoa also were observed in one year age. The development of seminiferous structure were observed continiued with the age. The results showed that, the perfect spermatogenesis and production of spermatozoa is observed in one year age, which it seems similar to cow (10-11 monthsage).