Evaluation of Antifungal activity of new derivatives of Imidazole using Colorimetric method

The incidence of invasive fungal infections has been increasing for two decades. Amatter of concern in the treatment of fungal infections is the limited number of efficacious antifungal drugs. Many available drugs lead to the development of resistance, In order to seek new antifungal agents we assessed the antifungal activity of newly synthesized Imidazol compounds by a colorometric method. In this experimental study antifungal activity of the new Imidazol compounds against Candida albicans, Saccharomyces cervisiae, Aspergillus niger, Microsporum gypseom was investigated by colorometric method and results were compared to microdilution ones. 2-hydroxyphenacyl-azole and 2- hydroxyphenacyl-azolium compounds have been identified as a new class of azole antifungal agents with a good spectrum of activity. The colorimetric method is a simple microtiter method for determining the susceptibility of species of fungal against antifungal agents. Most derivatives showed significant in vitro antifungal activities against tested fungi with low MIC (minimum inhibitory concentration) values included in the range of 0.25-32 µg/mL comparable to the reference drug Fluconazole.