Surface Processing of Cellulose and PVDF Supports for Immobilization of Antibody for Diagnostic Application

The immobilization of antibodies is one of the most important steps for the construction of biological diagnostic systems. Cellulose and PVDF are commonly used as supports for antibody immobilization despite their low efficiency. This study investigates the activation process of these supports in order to improve their immobilization capability. So as to apply a more operative immobilization of antibody, cellulose and PVDF were initially treated by cyanogen bromide and methanol respectively. In cellulose support which was treated by cyanogen bromide, the –OH groups constructed a cyclic imidocarbamate which is disposed to react with the amine groups of the antibody. In PVDF which was activated by methanol, hydrophilic property and surface adsorption were increased. After the activation procedure, anti-human antibody was immobilized on both supports and the immobilization quality was evaluated. The results showed that the applied changes on the supports had promising effects on the antibody capturing capacity. It was also shown that antibodies were more immobilized on the cellulose supports in comparison to PVDF. The activation processes can alter the physiochemical features of the support such as capillarity, flexibility, surface charges and delicacy, which may intensively influence the quality of immobilization.