Genetic Diversity of some Grapevine Cultivars of Khorasan Razavi Province Based on RAPD and ISSR Markers

Genetic variation of fifteen grapevine cultivars including six Khorasan native cultivars, eight imported cultivars from Torkamanstans and one Vitis muscadiana cultivar was studied using RAPD and ISSR markers. PCR amplification of DNA, using seven primers for RAPD and ISSR analysis, produced 59 and 58 bands, respectively that could be scored in all genotypes. The average number of polymorphic bands per RAPD and ISSR primers were 8.4 and 8.2, respectively. The RAPD and ISSR data were combined for UPGMA cluster analysis. Dendrogram based on genetic distance (UPGMA) segregated fifteen grapevine cultivars into two main clusters. Torkaman cultivars formed cluster I and native Khorasan cultivars with one cultivar of V. muscadiana were grouped together in cluster II. Based on Jaccard’s similarity coefficient,the highest similarity was observed between the Torkaman 6 and Torkaman 8 and the lowest similarity between Torkaman 3 and Sahebi Neyshabour cultivars.