Study of Genetic Diversity of Flue-Cured Tobacco)Nicotiana Tabacum L.) Genotypes using ISSR and Retrotransposon Markers

The first step in breeding programs is detecting the genetic diversity in breeding materials. Molecular markers help to reduce costs and time in breeding programs. Genetic variation in 49 genotypes of tobacco were studied using 12 ISSR markers, 3 Retrotransposon markers and the combination of ISSR and Retrotransposons. One hundred forty seven polymorphic bands were produced using 16 primers. Tos-2 primer with 16 followed by UBC811 and TOS-1 with 14 bands had the maximum and UBC825 and TOS- 3 with 4 band had the minimum polymorphic bands. PIC value varied between 0.27 to 0.46 and MI between 1.15 to 5.87. Principal Coordinates Analysis showed that the aforementioned values could explain total variation of 16.75 percent. In this study, cluster analysis using UPGMA, placed 49 genotypes in five cluster groups. Groups include 9, 4, 1, 13 and 22 genotypes, respectively. Discriminate Function via Fisher's linear (79.6 percent) confirmed the validity of clustering analysis result.