Mycobiota of volunteer plants in Hamedan province (W Iran)

Out of 163 samples of suspected volunteer plants to fungal infection collected from different areas of Hamedan province (W Iran)، 49 isolates of the anamorphic fungi were identified according to morphological and molecular features. Based on these، 13 species were identified and classified in six genera. Most of them belonged to Cladosporium، Ascochyta and Phoma، respectively. Among them، Embellisia astragali، E. tellustris، Phoma herbicola and Septoria sonchi on Scorzonera cana، Cirsium arvensis، Taraxacum sp. and Sonchus oleraceous are new records for the mycoflora of Iran. To confirm morphological identification، ITS regions (ITS1، ITS2 & 5. 8 S gene) of ribosomal DNA from these species were sequenced. ITS sequences of different species of Phoma and Embellisia were very diverse.