In Vitro Propagation of Primrose (Primula Acaulis L.), Via Shoot Tip Explants

This study carried out on commercial primrose (Primula acaulis L.). The aim of the present study was to establish an efficient and reproducible protocol for in vitro propagation of P. acaulis from shoot tips. First of all seeds were cultured on MS (Murashig & Skoog) medium without plant growth regulators. Afterwards, the seedling-derived shoot tips were cultured on MS basal medium, containing various concentrations of 6-benzylaminopurine (BA) and thidiazuron (TDZ) in combination with various concentrations of 1-naphthaleneacetic acid (NAA). The experiments was carried out in two factorial design on the basis of completely randomize design. The result showed that the frequency of shoot regeneration was 0 to 100% in the first experiment and 30 to 100% in the second one. The highest shoot regeneration in first and second experiments, considerably affected by MS media containing 1 mgL–l BA and also 0.4 mgL–l TDZ, respectively. Regenerated shoots easily rooted without the necessity for transfer to a rooting medium. Number of roots significantly affected by media supplemented with 3 mgL-1 BA + 0.8 mgL-1 NAA and 0.4 mgL-1 TDZ + 0.8 mgL-1 NAA. Finaly, plantlets were hardened-off and transferred to soil for further growth.