Effect of Male Parent on Embryo Rescue of Three Seedless Grapevine (Vitis vinifera L.) Cultivars

The study was conducted to investigate the effect of male parent on embryo rescue of three seedless cultivars, Flame Seedless, Yaghooti and Askary. Pistils of female parents were emasculated and then were pollinated with pollen of Bidaneh Sefid (seedless), Dizmary or Mehdikhani (seeded) and self-pollinated as control. Forty days after full bloom, berries were harvested, ovules were excised and cultured on Nitsch and Nitsch medium containing 1 µM GA3, 10 µM IAA and 2 g/l activated charcoal. Germinated ovules were transferred to 1/2 MS medium. Male parent had significant effect on ovule blackening and ovule germination and no effect on callus formation, ovule growth or ovule collapse. The percentage of blackened ovules was increased when Bidaneh Sefid was used as male parent. However, it reduced the number of germinated ovules in compared with those from self-pollination. The effects of seeded male parents varied for different female parents. Compared with self-pollination, Dizmary increased ovule germination but Mehdikhani had no effect on ovule germination. It was found that embryo development and germination varied greatly with both female and male parent genotypes.