Optimization and evaluation of various nitrogen sources on growth of selected oil degrading strains, isolated from the southern shore of the Caspian
Author(s):
Abstract:
marine pollution are factors that are constantly causing contamination of water and beach areas. Oil spills from ships at sea, oil wells, facilities, environmental and economic damage to coastal and so many are looking for [1, 2]. The aim of this project, and optimization of crude oil degrading microorganisms isolated from Svyhhayy and evaluate the effect of various nitrogen sources on growth of selected strains in relation to oil pollution has been eliminated. samples were prepared from different parts of the coast. enrichment of oil degrading bacteria in the soil bed was designed and implemented. enrichment at the end of the process, the suspension liquid medium, were cultured on solid medium. With solid culture s, pure strains were obtained from single colonies. Effect of different sources of nitrogen for 4 types of different nitrogen sources, ammonium nitrate (NH4NO3), sodium nitrate (NaNO3), urea (CONH2CO), ammonium chloride(NH4Cl) in mineral medium was used. Then the medium that strains The comments are inoculated, Temperature in a shaker incubator at 27 ° C were, Strains to grow in environments that ships were in different nitrogen sources, are compared. Daily rate of bacterial growth and to help Uv-Vis devices were measured. Showed that strain S6-R1-1, the nitrogen in ammonium nitrate (NH4NO3) better than the other sources used. The ammonium nitrate, sodium nitrate, ammonium chloride, after which appropriate resource is suitable nitrogen source. Urea is the last category.
Keywords:
Language:
English
Published:
Journal of Biodiversity and Ecological Sciences, Volume:2 Issue: 1, 2012
Page:
80
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