Preparation and investigation of in vitro efficacy of epigallocatechin gallate loaded- nanoliposomes against resistant strain of Staphylococcus aureus

Staphylococcus aureus is resistant to many commonly used antibiotics. Different studies have shown that herbal ingredients such as epigallocatechin gallate (EGCG), and a delivery drug system such as nanoliposomes could kill antibiotic-resistant bacteria. The objective of this study was to prepare and evaluate in vitro anti- staphylococcus aureus efficacy of prepared EGCG-loaded nanoliposomes. EGCG-loaded nanoliposomes with negative surface charge were prepared by reverse phase evaporation method. For preparation of nanoliposomes lecithin, cholesterol and dicetyl phosphate were used. Then, the EGCG encapsulation efficacy was measured by spectrophotometric. The minimum inhibitory concentration (MIC) of EGCG-loaded nanoliposomes for resistant strain of Staphylococcus aureus were determined by broth dilution method. Ultimately, the killing rate of Staphylococcus aureus was exposed to MIC concentration of EGCG in free and nanoliposomal form was analyzed. The encapsulation efficacy for EGCG-loaded nanoliposomes was to 75%±0.31. The MIC of free and nanoliposomal form of EGCG against resistant strain of Staphylococcus aureus was to 50 and 25 mg/l, respectively. Also the killing rate of EGCG-loaded nanoliposomes was higher than those of free EGCG at identical times. Our data suggest that the prepared EGCG-loaded nanoliposomes because of high effectiveness would be a choice appropriate for treatment of Staphylococcus aureus infections.