Comparative study of histomorphometric and histopathological effects of topical ointment (OTC) and oxytetracycline spray on wound healing in equine model

In this study the comparative effects of topical ointment (OTC) and oxytetracycline spray on the hystomorphological and histopathological process of wound healing in equine model were investigated. This experimental study was done on eight male donkeys which divided in three groups. The groups were treated with topical ointment 3% (OTC), oxytetracycline spray and control group (washing with normal saline) for 28 days. After induction of anesthesia by Ketamine and Xylazin hydrocholoride, 3×10 cm of skin of the lateral surface of the metacarp and metatars incised and removed by aseptic surgical method in rectangular shape. Healing process of the wounds measured daily by taking digital photographs and analysis these photos and determining the size of the wound area. Histopathological samples were prepared in days 7, 14, 21, and 28 days after treatment and were studied after staining. The obtained results from morphological, histopathological and statistical findings showed a better effect of oxytetracycline spray comparing to using topical ointment (OTC) on wound healing in donkeys.