Antioxidant activity of the aerial parts extract of Bidens bipinnata L. at fruiting stage

Bidens bipinnata L. belongs to the family Asteraceae (Compositae) and genus of Bidens. In order to study the antioxidant activity, total phenolic and flavonoid content of methanolic extract of Bidens bipinnata L., were investigated. For this purpose, after full identification of the species and determination of its habitat in Alborz province, aerial parts of the plant were collected at fruiting stage. In this research, three methods including DPPH, ABTS, and FRAP were applied to measure flavonoid and total phenolic content of the species. Results showed that for methanol extract of the aerial parts of Bidens bipinnata, percentage of radical inhibition was 82.13 μg/ml (IC50> 100 μg/ml). The amount of ferrous sulfate reagent was 112.66 μM/g based on the dry weight of plant in FRAP method. According to the results of ABTS method, the amount of Ascorbic Acid, total flavonoids and total phenolic compounds were respectively calculated based on the dry weight of plant as 9.15 mg and also the amount of total flavonoids in dry weight of plant using rutin reagent was determined 12.21 mg/g and the amount of total phenolic compounds of the extract using Gallic Acid reagent was determined 2.88 mg/g.