Micropropagation of Iranian Populus alba species

Iran is considered to be amongst the low forest cover countries. The development of poplar farming can be effectively helpful for conserving and restoring the forests. Populus alba is one of the useful fast growing species with industrial wood which can significantly contribute to development of wood farming. Unfortunately, the seedling production of elite trees by classic techniques could not be enough for papules farming demand. This work was done for micropropagation of Iranian's selected elite Populus alba trees for demand of papules farmers and, also, for cryopreservation program on the shoots of Populus alba trees collected from the National Botanical Garden of Iran. The buds sterilized and settled in 8 treatments of hormones and culture mediums. The shoot induction rates recorded from 8 hormonal treatments and 5 replicates in a complete randomized design. The best shoot induction attributed to the basal culture medium of DKW combined with the hormones of 2iP, IBA and BA at a concentration of 0.3 mgl-1, 0.1 mgl-1 and 0.3 mgl-1, respectively. The best treatment for root growth gained in the DKW free hormone medium with 80% root production. Seedlings 100% survived in greenhouse after adaptation.