Evaluation of the Enterococcus faecalis role on inhibitory growth of staphylococcus aureus by production of a antimicrobial compound

The object of this research performance was Evaluation of the Enterococcus faecalis role on inhibitory growth of staphylococcus aureus by production of a antimicrobial compound. After the culture of Enterococcus faecalis with property of PTCC 1394 and ATCC 9854 in the BHI agar, centrifuging and isolation of microbial mass, that antimicrobial compound was purified by dialyze. Antimicrobial effect of present compound was evaluated on gram positive and gram negative indicator bacteries by well diffusion method. Inhibitory Concentration is determined by dilution method. The results of this research proved that Activity Unit, Specific activity, purification factor increased after dyalisis. Total protein, Concentration, Total activity, amount of total protein decreased after dyalisis. Produced the lowest inhibitory concentration by antimicrobial compound was on Staphylococcus aureus. In attention to achieved results of this antimicrobial compound, Enterococcus faecalis with property of PTCC1394 can play a important role as a probiotic in food industry by producing of a anitimicrobial compound.