Phylogenetic identification of nitrogen-fixing bacteria isolated from the rhizosphere of asparagus plants using 16s rRNA and the effect of zinc on isolated strains

According to the role of microorganisms in nitrogen stabilizer in the fertility of the soil and growth and development of plants، the use of chemical fertilizers zinc sulfate (ZnSo4) as micronutrient index in agricultur. Due to the toxic effects of zinc metal، the aim of this research، isolation and identification of nitrogen stabilizer microorganisms of asparagus rhizosphere and the effect of toxic zinc metal on the growth of bacteria. In order to isolate microorganisms in nitrogen stabilizer، soil samples from the asparagus rhizosphere preparation in vitro lacks a nitrogen with a temperature of 30 ° c for 48 hours، was cultured، phylogenetic identify it using 16s rRNA do accepted and the MIC strain isolated in different concentrations of zinc metal was determined. Rhizosphere soil samples from the plant among the asparagus a strain of bacteria was isolated، check the phylogenetic it using 16s rRNA species of Acinetobacter calcoaceticus was detected and isolated in disallowed MIC concentration 52. 32 ppm zinc metal was evaluated. One of the rhizosphere nitrogen stabilizer strains in the plant asparagus، bacterium Acinetobacter calcoaceticus، increase the intake of zinc metal was microorganisms cause damage and the maximum allowable amount of zinc metal in this review، the concentration is less than the 52. 32 ppm.